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Kinetochore and microtubules in two members of Chlorophyceae, Cladophora fracta and Spirogyra majuscula

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Abstract

Evidence is presented for the existence of a localised kinetochore with stratified fine structure in Cladophora and in Spirogyra. In the latter, there is the possibility of two kinetochores on the longer chromosomes. There is no evidence for a diffuse kinetochore. The nucleolus persists during mitosis in Cladophora on the nucleolar organising chromosomes, the granular material being lost from it very largely during metaphase and anaphase but the fibrillar material remaining. The persistent nucleolar material at metaphase and anaphase in Spirogyra is not attached to the nucleolar organising chromosomes but accumulates around all the chromosomes and chromatids, the microtubules of the spindle at anaphase passing through and possibly attaching to this nucleolar material and possibly assisting in the movement of the chromatids which are embedded within it.

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References

  • Bajer, A.: Chromosome movement and fine structure of mitotic spindle. In: Aspects of cell motility. 22nd Symp. Soc. exp. Biol. 285–310 (1968a)

  • Bajer, A.: Behaviour and fine structure of spindle fibres during mitosis in endosperm. Chromosoma (Berl.) 25, 249–281 (1968b)

    Google Scholar 

  • Bauer, H.: Die kinetische Organisation der Lepidopteren-Chromosomen. Chromosoma (Berl.) 22, 101–125 (1967)

    Google Scholar 

  • Bech-Hansen, C.W., Fowke, L.C.: Mitosis in Mougeotia sp. Canad. J. Bot. 50, 1811–1816 (1972)

    Google Scholar 

  • Braselton, J.P.: The ultrastructure of the non-localized kinetochores in Luzula and Cyperus. Chromosoma (Berl.) 36, 80–99 (1971)

    Google Scholar 

  • Brinkley, B.R., Stubblefield, E.: The fine structure of the kinetochore of a mammalian cell in vitro. Chromosoma (Berl.) 19, 28–43 (1966)

    Google Scholar 

  • Buck, R.C.: Mitosis and meiosis in Rhodnius prolixus: The fine structure of the spindle and diffuse kinetochore. J. Ultrastruct. Res. 18, 489–501 (1967)

    Google Scholar 

  • Davies, D.R., Plaskitt, A.: John Innes Annual Report, p. 64 (1970)

  • Geitler, L.: Über die Kernteilung von Spirogyra. Arch. Protistenk. 71, 10–18 (1930)

    Google Scholar 

  • Geitler, L.: Vergleichende Untersuchungen über den feineren Kern- und Chromosomenbau der Cladophoraceen. Planta (Berl.) 35, 530–578 (1936)

    Google Scholar 

  • Girbardt, M.: Ultrastructure and dynamics of the moving nucleus. Symp. Soc. exp. Biol. 22, 249–260 (1968)

    Google Scholar 

  • Godward, M.B.E.: The life cycle of Stigeoclonium amoenum. New Phytol. 41, 293–301 (1942)

    Google Scholar 

  • Godward, M.B.E.: The iron alum acetocarmine method for algae. Nature (Lond.) 161, 203 (1948)

    Google Scholar 

  • Godward, M.B.E.: The “diffuse” centromere or polycentric chromosomes in Spirogyra. Ann. Bot. 18, 143–156 (1954)

    Google Scholar 

  • Godward, M.B.E.: Meiosis in Spirogyra crassa. Heredity 16, 53–62 (1961)

    Google Scholar 

  • Godward, M.B.E.: The Chlorophyceae. In: The chromosomes of the Algae (M.B.E. Godward, ed.), p. 1–77. London: Edward Arnold Ltd. 1966

    Google Scholar 

  • Godward, M.B.E., Jordan, E.G.: Electron microscopy of the nucleolus of Spirogyra britannica and Spirogyra ellipsospora. J. roy. micr. Soc. 84, 347–360 (1965)

    Google Scholar 

  • Heath, I.B., Greenwood, A.D.: Electron microscopic observations of dividing somatic nuclei in Saprolegnia. J. gen. Microbiol. 53, 287–289 (1968)

    Google Scholar 

  • Heath, I.B., Greenwood, A.D.: Centriole replication and nuclear dension in Saprolegnia. J. gen. Microbiol. 62, 139–148 (1970)

    Google Scholar 

  • Heywood, P., Godward, M.B.E.: Centromeric organization in the Chloromonadophycean alga Vacuolaria virescens. Chromosoma (Berl.) 39, 333–339 (1972)

    Google Scholar 

  • Jokelainen, P.T.: The ultrastructure and special organization of the metaphase kinetochore in mitotic rat cells. J. Ultrastruct. Res. 19, 19–44 (1967)

    Google Scholar 

  • Jordan, E.G., Godward, M.B.E.: Some observations on the nucleolus in Spirogyra. J. Cell Sci. 4, 3–15 (1969)

    Google Scholar 

  • Krishan, A., Buck, R.C.: Structure of the mitotic spindle in L strain fibroblasts. J. Cell Biol. 24, 433–444 (1965)

    Google Scholar 

  • Leedale, G.F.: The nucleus in Euglena. In: The biology of Euglena. (D.E. Buetow, ed.). New York: Academic Press 1968

    Google Scholar 

  • Luykx, P.: The structure of the kinetochore in meiosis and mitosis in Urechis eggs. Exp. Cell Res. 39, 643–657 (1965)

    Google Scholar 

  • Marchant, H.J., Pickett-Heaps, J.D.: Ultrastructure and differentiation of Hydrodictyon reticulatum. Aust. J. biol. Sci. 23, 1173–1186 (1970)

    Google Scholar 

  • Molè-Bajer, J.: Fine structural studies of apolar mitosis. Chromosoma (Berl.) 26, 427–448 (1969)

    Google Scholar 

  • Patel, R.J.: A cytological and taxonomic study of British Cladophorales. Ph. D. Thesis, London University 1961

  • Pickett-Heaps, J.D.: Ultrastructure and differentiation in Chara sp. II. Mitosis. Aust. J. biol. Sci. 20, 883–894 (1967)

    Google Scholar 

  • Pickett-Heaps, J.D.: Variation in mitosis and cytokinesis in plant cells: its significance in the phylogeny and evolution of ultrastructural systems. Cytobios 5, 59–77 (1972a)

    Google Scholar 

  • Pickett-Heaps, J.D.: Cell division in Tetraedron. Ann. Bot. 36, 693–701 (1972b)

    Google Scholar 

  • Pickett-Heaps, J.D., Fowke, L.C.: Cell division in Oedogonium I. Aust. J. biol. Sci. 22, 857–894 (1969)

    Google Scholar 

  • Pickett-Heaps, J.D., Fowke, L.C.: Cell division in Oedogonium II. Aust. J. biol. Soi. 23, 71–92 (1970)

    Google Scholar 

  • Ris, H., Kubai, D.: Mitosis in the parasitic Dinoflagellate Syndinium globiforme. Abstracts, 12th Ann. Meeting Amer. Soc. Cell Biol: in J. Cell Biol. 55, 217a (1972)

  • Robinow, C.F.: Observations on cell growth, mitosis, and division in the fungus Basidiobolus ranarum. J. Cell Biol. 17, 123–151 (1963)

    Google Scholar 

  • Robinow, C.F., Marak, J.: A fibre apparatus in the nucleus of the yeast cell. J. Cell Biol. 29, 129–151 (1966)

    Google Scholar 

  • Sengel, A., Stoebner, P.: Golgi origin of tubular inclusions in endothelial cells. J. Cell Biol. 44, 223–227 (1970)

    Google Scholar 

  • Sinha, J.P.: Cytological and cultural study of some members of Cladophorales and Oedogoniales. Ph. D. Thesis, London University 1958

  • Tanaka, K.: Mitosis in the fungus Basidiobolus ranarum as revealed by electron microscopy. Protoplasma (Wien) 70, 423–440 (1970)

    Google Scholar 

  • Turner, F.R.: An ultrastructural study of plant spermatogenesis. Spermatogenesis in Nitella. J. Cell Biol. 37, 370–393 (1968)

    Google Scholar 

  • Wilson, H.J.: Endoplasmic reticulum and microtubule formation in dividing cells of higher plants — a postulate. Planta (Berl.) 94, 184–190 (1970)

    Google Scholar 

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Authors and Affiliations

  1. Department of Plant Biology and Microbiology, Queen Mary College, London

    Sanjida Mughal & M. B. E. Godward

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  1. Sanjida Mughal
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  2. M. B. E. Godward
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Mughal, S., Godward, M.B.E. Kinetochore and microtubules in two members of Chlorophyceae, Cladophora fracta and Spirogyra majuscula . Chromosoma 44, 213–229 (1973). https://doi.org/10.1007/BF00329118

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  • DOI: https://doi.org/10.1007/BF00329118

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