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Size and organization of a multigene family encoding phaseolin, the major seed storage protein of Phaseolus vulgaris L.

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Summary

Solution hybridization kinetics and genomic nitrocellulose blot hybridization analyses show that the Phaseolus vulgaris L. (French bean) storage proteins (phaseolins) are encoded as a small, homologous, multigene family consisting of approximately seven members. Restriction endonuclease site mapping (EcoRI, BamHI, and BglII) of DNA regions flanking the phaseolin genes has shown that the gene family can be divided into at least three characteristic fragment size classes. Clones representative of two of these phaseolin gene classes have been isolated from a λ1059 phage library.

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Authors and Affiliations

  1. Agrigenetics Advanced Research Laboratory, 5649 East Buckeye Road, 53716, Madison, WI, USA

    Duncan R. Talbot, Michael J. Adang, Jerry L. Slightom & Timothy C. Hall

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  1. Duncan R. Talbot
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  2. Michael J. Adang
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  3. Jerry L. Slightom
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  4. Timothy C. Hall
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Communicated by R. Goldberg

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Talbot, D.R., Adang, M.J., Slightom, J.L. et al. Size and organization of a multigene family encoding phaseolin, the major seed storage protein of Phaseolus vulgaris L.. Molec Gen Genet 198, 42–49 (1984). https://doi.org/10.1007/BF00328698

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  • DOI: https://doi.org/10.1007/BF00328698

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