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A reinvestigation of the nucleolus-organizing regions in the salivary gland nuclei of Drosophila melanogaster

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Abstract

Comparisons were made of the morphology of the proximal region of the salivary gland X-chromosome of D. melanogaster following a number of different staining procedures. Azur B was found to be the most satisfactory staining method for identification of the nucleolus. The states of eu- and heterochromatization (sensu Prokofyeva-Belgovskaya) of the bands in the proximal region—particulary striking in the mirror-image duplication of the R(1)2, ring-X, chromosome—contribute to the variability in the banding-pattern, and consequently the refractoriness of this region to cytological investigation. No nucleolus was ever found to be associated with the group of bands presumed to be the Y-chromosome.

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References

  • Arcos-Terán, L., Beermann, W.: Changes of DNA replication behavior associated with intragenic changes of the white region in Drosophila melanogaster. Chromosoma (Berl.) 25, 377–391 (1968).

    Google Scholar 

  • Barigozzi, C.: A general survey on heterochromatin. Port. Acta biol. A, R. B. Goldschmidt Vol., 593–620 (1951).

  • Barr, H. J., Plaut, W.: Comparative morphology of nucleolar DNA in Drosophila. J. Cell Biol. 31, C17-C22 (1966).

    Google Scholar 

  • Bauer, H.: Structure and arrangement of salivary gland chromosomes in Drosophila species. Proc. nat. Acad. Sci. (Wash.) 22, 216–222 (1936).

    Google Scholar 

  • Beermann, W.: Riesenchromosomen. Protoplasmatologia, Bd. VI/D, S. 1–161. Wien: Springer 1962.

    Google Scholar 

  • Bridges, C. B.: Salivary chromosome maps. J. Hered. 26, 60–64 (1935).

    Google Scholar 

  • —: Revised map of the salivary gland X-chromosome of Drosophila melanogaster. J. Hered. 29, 11–13 (1938).

    Google Scholar 

  • Brown, S. W.: Heterochromatin. Science 151, 417–425 (1966).

    Google Scholar 

  • Cooper, K. W.: Cytogenetic analysis of major heterochromatic elements (especially Xh and Y) in Drosophila melanogaster, and the theory of heterochromatin. Chromosoma (Berl.) 10, 535–588 (1959).

    Google Scholar 

  • Darlington, C. D., La Cour, L. F.: The Handling of Chromosomes, 4th ed. London: George Allen & Unwin, Ltd. 1962.

    Google Scholar 

  • Emmons, C. W.:The morphology of the nucleus in the salivary glands of four species of Drosophila (D. melanogaster, D. immigrans, D. funebris and D. subobscura. Z. Zellforsch. 26, 1–20 (1937).

    Google Scholar 

  • Frolova, S.: Struktur der Kerne in den Speicheldrüsen einiger Drosophila-Arten. [Russian and German summary.] Biol. Zhurn. 5, 271–292 (1936).

    Google Scholar 

  • —: Development of the giant nuclei of salivary gland type in different organs of Drosophila and their comparison with “metabolic” nuclei of larvae and imago. Biol. Zhurn. 7, 703–736 (1938).

    Google Scholar 

  • Gall, J. G., Cohen, E. H., Polan, M. L.: Repetitive DNA sequences in Drosophila. Chromosoma (Berl.) 33, 319–344 (1971).

    Google Scholar 

  • Gay, H.: Chromosome structure and function. Carnegie Inst. Washington, Yearbk. 62, 501–510 (1963).

    Google Scholar 

  • Griffen, A. B.: The obscure parts of the X chromosome in Drosophila melanogaster. Genetica 36, 105–113 (1965).

    Google Scholar 

  • Hannah, A.: Radiation-mutations involving the cut locus in Drosophila. Proc. 8th Intern. Congr. Genet. Hereditas (Lund.), Suppl., 588–589 (1949).

  • —: Environmental factors affecting elimination of the ring-X chromosome in Drosophih melanogaster. Z. indukt. Abstamm.- u. Vererb.-L. 86, 600–621 (1955).

    Google Scholar 

  • Hannah-Alava, A.: Cytogenetics of nucleolus-transpositions in Drosophila melanogaster. Molec. gen. Genet. 113, 191–203 (1971).

    Google Scholar 

  • Heitz, E.: Über α- und β-Heterochromatin sowie Konstanz und Bau der Chromomeren bei Drosophila. Biol. Zbl. 54, 588–609 (1934).

    Google Scholar 

  • Kaufmann, B. P.: The nucleolus-organizer regions in salivary gland chromosomes of Drosophila melanogaster. Z. Zellforsch. 28, 1–11 (1938).

    Google Scholar 

  • —: Induced chromosome rearrangements in Drosophila melanogaster. J. Hered. 30 179–190 (1939).

    Google Scholar 

  • Kroeger, H.: Microsurgery on cells with polytene chromosomes. In: Methods in cell physiology (D. M. Prescott, ed.) vol. 2, p. 61–92. NewYork: Academic Press 1966.

    Google Scholar 

  • Lindsley, D. L., Grell, E. H.: Genetic Variations of Drosophila melanogaster. Carnegie Inst. Wash. Publ. No 627 (1968).

  • Murgatroyd, L. B.: Some salient points in the preparation of a new methyl green pyronin stain and use in cytology and histology. Mikroskopie 18, 285–287 (1963).

    Google Scholar 

  • Nash, D., Plaut, W.: On the presence of DNA in larval salivary gland nucleoli in Drosophila melanogaster. J. Cell Biol. 27, 682–686 (1965).

    Google Scholar 

  • Nicoletti, B., Lindsley, D. L.: Translocations between the X and Y chromosomes of Drosophila melanogaster, Genetics 45, 1705–1722 (1960).

    Google Scholar 

  • Painter, T. S.: The morphology of the X-chromosome in salivary glands of Drosophila melanogaster and a new type of chromosome map for this element. Genetics 19, 448–469 (1934).

    Google Scholar 

  • —, Stone, W.: Chromosome fusion and speciation in Drosophila. Genetics 20, 327–341 (1935).

    Google Scholar 

  • Pelling, C.: Ribonukleinsäure-Synthese der Riesenchromosomen. Autoradiographische Untersuchungen an Chironomus tentans. Chromosoma (Berl.) 15, 71–122 (1964).

    Google Scholar 

  • Prokofyeva-Belgovskaya, A. A.: The structure of the Y-chromosome in the salivary glands of Drosophila melanogaster. Genetics 22, 94–103 (1937).

    Google Scholar 

  • —: Heterochromatization as a change in the chromosome cycle. (Russian with English summary.) Biol. Zhurn. 62, 93–124 (1945).

    Google Scholar 

  • —: Heterochromatization as a change of chromosome cycle. J. Genet. 48, 80–98 (1947).

    Google Scholar 

  • Ritossa, F. M., Spiegelman, S.: Localization of DNA complementary to ribosomal RNA in the nucleolus organizer region of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 53, 737–745 (1965).

    Google Scholar 

  • Rodman, T. C.: Morphology and replication of intranucleolar DNA in polytene nuclei. J. Cell Biol. 42, 575–582 (1969).

    Google Scholar 

  • Rudkin, G. T.: The structure and function of heterochromatin. In: Genetics Today. Proc. XI Intern. Congr. Genetics 1963 (S. J. Geerts, ed.) vol 2, p. 359–374. Oxford: Pergamon Press 1965.

    Google Scholar 

  • Schultz, J., Catcheside, D. G.: The nature of closed X-chromosomes in Drosophila melanogaster. J. Genet. 35, 315–320 (1938).

    Google Scholar 

  • Slizynski, B. H.: Deficiency effects of ultra-violet light in Drosophila melanogaster. Proc. roy. Soc. (Edinb.) 61B, 297–315 (1942).

    Google Scholar 

  • Sorsa, M.: Ultrastructure of the chromocentre heterochromatin in Drosophila melanogaster. Ann. Acad. Sci. fenn. A-IV, 146, 1–20 (1969).

    Google Scholar 

  • Swift, H.: Cytochemical techniques in nucleic acids. In: Nucleic acids (E. Chargaff and J. N. Davidson, eds.) vol. 2, p. 51–92. New York: Academic Press 1955.

    Google Scholar 

  • Tiniakov, G. G.: The inert regions and general morphology of the chromosomes in the salivary gland cells of Drosophila. Biol. Zhurn. 5, 753–802 (1936).

    Google Scholar 

  • Vincent, W. S., Miller Jr., O. L. (eds.): Intern. Symp.: The nucleolus, its structure and function. Nat. Cancer Inst. Monogr. 23 (1966).

  • Warters, M., Griffen, A. B.: The centromeres of Drosophila. Genetica 30, 152–167 (1959).

    Google Scholar 

  • Wimber, D. E., Steffensen, D.: Localization of 5 S RNA genes on Drosophila chromosomes by RNA-DNA hybridization. Science 170, 639–641 (1970).

    Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, University of Turku, Finland

    Yrjö Viinikka, Aloha Hannah-Alava & Pirkko Arajärvi

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  1. Yrjö Viinikka
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  2. Aloha Hannah-Alava
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  3. Pirkko Arajärvi
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Additional information

This investigation was supported in part by a U.S. Public Health Service Research Grant, GM 15009, from the Institute of General Medical Sciences of the National Institutes of Health, and in part by a grant from the Finnish National Research Council for Sciences.

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Viinikka, Y., Hannah-Alava, A. & Arajärvi, P. A reinvestigation of the nucleolus-organizing regions in the salivary gland nuclei of Drosophila melanogaster . Chromosoma 36, 34–45 (1971). https://doi.org/10.1007/BF00326420

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