Abstract
Male and female rats were dosed once a day for 2 days with injections of 1.5 mg Cd/kg. Formation of thiobarbituric acid reactive substances (TBA-RS) was significantly increased in male rat liver but not in the females. NADPH-dependent lipid peroxidation in vitro in microsomes derived from untreated rat liver was greater in males than in females. Furthermore, addition of cadmium (Cd) to microsomes isolated from male rat liver produced a dose-dependent potentiation of NADPH-dependent lipid peroxidation from low concentrations of Cd. In microsomes derived from females a significant increase in lipid peroxidation was observed only at high Cd concentrations. NADPH-dependent lipid peroxidation enhanced by Cd was greater in the males than in the females. These data suggest that a sex-related difference in the ability of Cd to induce lipid peroxidation in vivo in rat liver appears to be mediated partly through differences in hepatic microsomal NADPH-dependent lipid peroxidation.
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References
Backer ET (1969) Chloric acid digestion in the determination of trace metals (Fe, Zn and Cu) in brain and hair by atomic absorption spectrometry. Clin Chem Acta 24: 233–236
Chvapil M, Ryan JN, Zukoski CF (1972) Effect of zinc on lipid peroxidation in liver microsomes and mitochondria. Proc Soc Exp Biol Med 141: 150–153
Gabor S, Anca Z, Bordas E (1978) Cadmium-induced lipid peroxidation in kidney and testes. Effect of zinc and copper. Rev Roum Biochem 15: 113–117
Igarashi T, Satoh H, Ueno K, Kitagawa H (1983) Sex-related difference in the hepatic glutathione level and related enzyme activities in rat. J Biochem 93: 33–36
Johns CO, Finkes AJ, Alsberg CL (1923) Chronic intoxication by small quantities of cadmium chloride in the rat. J Pharmacol Exp Ther 21: 59–64
Kamataki T, Kitagawa H (1973) Effects of lipid peroxidation on activities of drug-metabolizing enzymes in liver microsomes of rats. Biochem Pharmacol 22: 3199–3207
Kato R, Takanaka A, Takayanagi M (1968) Studies on mechanism of sex difference in drug-oxidizing activity of liver microsomes. Jpn J Pharmacol 18: 482–489
Pederson TC, Aust SD (1972) NADPH-dependent lipid peroxidation catalyzed by purified NADPH-cytochrome C reductase from rat liver microsomes. Biochem Biophys Res Commun 48: 789–795
Pence DH, Miya TS, Schnell RC (1977) Cadmium alteration of hexobarbital actions: Sex-related differences in the rat. Toxicol Appl Pharmacol 39: 89–96
Pinto RE, Bartley W (1969) The effect of age and sex or glutathione reductase and glutathione peroxidase activities and on aerobic oxidation in rat liver homogenates. Biochem J 112: 109–115
Saito H, Shitomi K, Arikawa T, Saito T, Furuyama T, Yoshinaga K (1978) Health examination on people in the cadmium-polluted area. Kankyo Hoken Report 44: 175–177 (in Japanese, Association of Public Health)
Sagai M, Ichinose T, Oda H, Kubota K (1982) Studies on biochemical effect of nitrogen dioxide II. Cahnges of the protective systems in rat lungs and of lipid peroxidation by acute exposure. J Toxicol Environ Health 9: 153–164
Sasse D, Hoffman H (1982) Postnatal differentiation of sex-specific distribution patterns of G6Pase, G6PDH and ME in the rat liver. Histochemistry 75: 31–42
Sato M, Yamanobe K, Nagai Y (1983) Sex-related differences in cadmium-induced lipid peroxidation in the rat. Life Sci 33: 903–908
Schnell RC, Means JR, Roberts SA, Pence DH (1979) Studies on cadmium-induced inhibition of hepatic microsomal drug biotransformation in the rat. Environ Health Perspect 28: 273–279
Uchiyama M, Mihara M (1978) Determination of malonaldehyde precursor in tissue by thiobabituric acid test. Anal Biochem 86: 271–278
Yonaha M, Ohbayashi Y, Ichinose T, Sagai H (1982) Lipid peroxidation stimulated by mercuric chloride and its relation to the toxicity. Chem Pharm Bull 30: 1437–1442
Yoshida T, Suzuki Y, Hashimoto Y (1979) Sex-related effect of cadmium on hepatic cytochrome P-450, drug-metabolizing enzymes and δ-aminolevulinic acid synthetase and heme oxygenase in the rat. Toxicol Lett 4: 97–102
Yoshikawa H (1970) Preventive effect of pretreatment with low dose of metals on the acute toxicity of metals in mice. Ind Healths 8: 184–191
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Sato, M., Nagai, Y. Sex-related differences in NADPH-dependent lipid peroxidation induced by cadmium. Arch Toxicol 59, 156–159 (1986). https://doi.org/10.1007/BF00316325
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DOI: https://doi.org/10.1007/BF00316325