Abstract
In general, people may come in contact with mixtures of insecticides through domestic use, consumption of contaminated food or drinks, and/or living close to treated areas. We analyzed the toxic effects of diazinon on histological structure of liver and hematological parameters in male rats. DNA-damaging potential of diazinon was also investigated using the comet assay in blood cells and the micronucleus test in bone marrow. Two groups of six male rats orally received different amounts of diazinon: 1/50 and 1/25 LD 50 for 4 weeks (5 day/week). The present study showed that diazinon caused hypertrophy of sinusoids, central vein, and portal triad, in addition to the formation of oedema, vacuoles, hemorrhage, necrosis, and lymphoid infiltration in rats’ liver. A significant decrease in red blood cells, hemoglobin, hematocrite levels, and platelet counts was observed in the treated groups. However, the white blood cell count increased. Micronucleus test results revealed aneugenic effects of diazinon. Furthermore, we noticed an increase in comet tail length in treated groups. So, the comet assay confirmed the genotoxic potential of diazinon in vivo. On the assumption that all alterations observed in rats could be observed in human, it is necessary to raise the awareness about the health risk posed by this insecticide.
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Acknowledgments
This work was supported by funds allocated to the Research Unit of Histology and Genetic UR 12 ES 10 by the “Ministère Tunisien de l’Enseingement Supérieur et de la Recherché Scientifique”. The authors thank the personnel of the Laboratory of hematology, University Hospital of Monastir, in their help in the determination of hematological parameters. We also thank the reviewers for their helpful comments and Prof. Moncef Rassas for English proofreading.
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Ezzi, L., Haouas, Z., Salah, I.B. et al. Toxicopathic changes and genotoxic effects in liver of rat following exposure to diazinon. Environ Sci Pollut Res 23, 11163–11170 (2016). https://doi.org/10.1007/s11356-016-6314-0
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DOI: https://doi.org/10.1007/s11356-016-6314-0