Abstract
Adult female rats were fed with a diet containing hexachlorobenzene to induce a porphyria. 5α/5β-ratios of androstane steroids in blood were 0.61 ± 0.13 in porphyric rats and 1.05 ± 0.35 in normal rats. Etiocholanolone treatment of rats was ineffective in generation of a porphyria. The activity of microsomal glucuronyltransferase increased from 0.20 ± 0.04 mU/mg to 0.77 ± 0.23 mU/mg by this treatment. Administration of Flutamide (4′-nitro-3′-trifluoromethylisobutyranilide) to porphyric rats resulted in a decline of porphyrin excretion by 55%. The hepatic NADH-5β-reductase was strongly inhibited by this drug, whereas NADPH-5β-reductase displayed a slightly increased activity. These findings are further evidence for the involvement of 5β-steroids in the formation of porphyria.
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Abbreviations
- HCB:
-
Hexachlorobenzene
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The results presented are part of C. Tyrell's doctoral thesis
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Graef, V., Golf, S.W. & Tyrell, C. Further evidence for the participation of 5β-steroids in the development of a porphyria induced by hexachlorobenzene. Arch Toxicol 50, 233–239 (1982). https://doi.org/10.1007/BF00310855
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DOI: https://doi.org/10.1007/BF00310855