Summary
The brain of adult Rana temporaria has been investigated with the fluorescence microscopic technique of Falck and Owman (1965). In the pars ventralis tuberis cinerei, in the median eminence and in the pars intermedia of the hypophysis of normal animals, numerous monoaminergic nerve fibres were identified. In the pars ventralis tuberis cinerei, monoamine-containing perikarya have been found, neither in normal, untreated animals, nor in drug-treated animals. In the neural isolated hypothalamic islands, consisting of the pars ventralis tuberis cinerei + median eminence-hypophysis, and of which the normal blood supply had been preserved, no monoamine-containing perikarya have been observed. Moreover, in these islands, the monoaminergic nerve fibres had completely disappeared.
From the present results and from corresponding previous electron microscopic observations, it is concluded that:
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1.
The pars ventralis of the tuber cinereum of Rana temporaria does not contain monoaminergic perikarya.
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2.
The monoaminergic fibres which, in normal animals, are present in the pars ventralis tuberis, in the median eminence and in the pars intermedia of the hypophysis originate from monoamine-producing cells of which the perikarya must be located outside the pars ventralis tuberis cinerei.
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3.
The fibres of the tubero-hypophysial neurosecretory system and the monoaminergic tubero-hypophysial nerve fibres are separate, transporting and secreting different products.
From the present results and from our previous observations, it is inferred that the tuberohypophysial neurosecretory cells identified in Rana temporaria must be adenohypophysiotropic neurohormone producing neurosecretory cells.
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Of the results of this investigation, a preliminary note has been published (Dierickx et al., 1972).
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Dierickx, K., Goossens, N. & Vandenberghe, M.P. Identification of adenohypophysiotropic neurohormone producing neurosecretory cells in Rana temporaria . Z.Zellforsch 143, 93–106 (1973). https://doi.org/10.1007/BF00307453
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DOI: https://doi.org/10.1007/BF00307453