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Détection par immunofluorescence des cellules corticotropes et mélanotropes dans l'hypophyse de la grenouille, Rana temporaria L., au cours du développément

Detection of corticotropic and melanotropic cells in the hypophysis of the frog, Rana temporaria, in the course of the development

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Summary

During the embryonic and larval developmental stages of the frog, Rana temporaria L, anti-β 1–24, α 17–39 corticotropine, α and β MSH antibodies were used to define, with immunofluorescence technique, the appearence of corticotropic and melanotropic cells.

A very small number of fluorescent corticotropic cells appears for the first time during the embryonic stage (10 mm), just before the differentiation of the pars intermedia. The cells are small, their large nucleus is surrounded by a fine rim of fluorescent cytoplasm.

During premetamorphic stage, the anti-ACTH antibodies (anti-β 1–24 and anti-α 17–39 corticotropine) reveal more fluorescent cells in the whole pars distalis. The pars intermedia cells can also be visualized by both antisera.

At the end of prometamorphosis and during climax the corticotropic cells show a more precise localization. As in adult frog pars distalis, they are concentrated in the rostral half of the lobe. With the same technique anti-α and β MSH antibodies reveal only the cells of the pars intermedia. No other cell type of the pars distalis reacts with these antibodies. This technique has the advantage to show that the ACTH and the MSH cells appear very early during the embryonic life.

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Authors and Affiliations

  1. Laboratoire de Cytologie Animale, Université Louis Pasteur, Strasbourg, France

    Jeannine Doerr-Schott

  2. Institut National de la Recherche Agronomique Station de Physiologie de la Reproduction Nouzilly, France

    Maurice P. Dubois

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  1. Jeannine Doerr-Schott
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  2. Maurice P. Dubois
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Doerr-Schott, J., Dubois, M.P. Détection par immunofluorescence des cellules corticotropes et mélanotropes dans l'hypophyse de la grenouille, Rana temporaria L., au cours du développément. Z.Zellforsch 142, 571–580 (1973). https://doi.org/10.1007/BF00306716

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  • DOI: https://doi.org/10.1007/BF00306716

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