Summary
A variety of conditions caused both, the f+ antigen and fr-receptor sites of E. coli strains to appear or disappear simultaneously: Both characters were lost after temperature-downshift, they were recovered after temperature-upshift; fr-refractory mutants failed to absorb f+ antibodies. Strains, genetically labelled with an Flac+ episome permitted the differentiation of an episomal and a chromosomal lesion, both of which affected the receptor site for fr, and at least one of which (episomal lesion) affected the formation of the f+ antigen as well. (In addition, strains lacking fr receptors had exhibited a significant reduction of their capacity to form recombinants.) The results provide strong correlative evidence for the identity of the f+ antigen and the cellular receptor for fr. The results provide additional evidence for the identity of the phage- and the mating-specific sites on E. coli.
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Knolle, P., Ørskov, I. The identity of the f+ antigen and the cellular receptor for the RNA phage fr. Molec. Gen. Genetics 99, 109–114 (1967). https://doi.org/10.1007/BF00306463
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DOI: https://doi.org/10.1007/BF00306463