Summary
The cholinesterase activity in the motor endplate of the striated muscle from frog and rat was investigated by the copper-ferrocyanide method of Karnovsky (1964). After an incubation time of ca. 4 minutes at 20° C the precipitate appears in the synapse. In it only the basement membrane is marked, while the prae- and subsynaptic membranes remain free. In the connective tissue above the motor endplate copper-ferrocyanide also precipitates. After addition of eserine-salicylate (10−4 M) to the washing buffer before the incubation the required incubation time is prolonged. Not only the basement membrane but also prae- and subsynaptic membranes then show on their external layer the copper-ferrocyanide precipitate. On the contrary it is absent in the connective tissue above the motor endplate.
Zusammenfassung
Die Cholinesteraseaktivität in der motorischen Endplatte der quergestreiften Muskulatur von Frosch und Ratte wurde durch die Kupferferrocyanidmethode von Karnovsky (1964) untersucht. Nach etwa 4 min Inkubationszeit bei 20° C tritt der erste Niederschlag in der Synapse auf. In ihr ist nur die Basalmembran markiert, während die prae- und die subsynaptische Membran frei bleiben. Im Bindegewebe über der motorischen Endplatte hat sich ebenfalls Kupferferrocyanid abgelagert. Nach Zugabe von Eserinsalicylat (10−4 M) zum Waschpuffer vor der Inkubation wird die erforderliche Inkubationsdauer wesentlich verlängert. Zusätzlich zur Basalmembran zeigen jetzt auch prä- und subsynaptische Membran auf ihre Außenseite einen Kupferferrocyanidniederschlag. Dagegen fehlt er im Bindegewebe über der Synapse.
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Bogusch, G. Zur Lokalisation der Cholinesteraseaktivität in der motorischen Endplatte mit der Kupferferrocyanidmethode. Histochemie 33, 39–46 (1972). https://doi.org/10.1007/BF00304224
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DOI: https://doi.org/10.1007/BF00304224