Summary
This report focuses on the application of enzymatic digestion techniques in electron microscopy to the study of the nuclear spindle poles of Ascomycetes and more specifically presents observations concerning the cytochemical characteristics of the plaques. Evidence is presented indicating that the plaques contain proteins digestable by pronase and trypsin (more than by pepsin), perhaps a low amount of RNA and that they also contain DNA as shown by their sensitivity to DNase and their Feulgen-positive reaction.
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We are grateful to Prof. J. André in whose laboratory all electron microscopy observations were made and to Prof. G. Rizet and Dr. L. Lewis for suggestions and reading the manuscript. This work was supported by grants from the C.N.R.S. (L.A. 86).
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Zickler, D. Evidence for the presence of DNA in the centrosomal plaques of Ascobolus . Histochemie 34, 227–238 (1973). https://doi.org/10.1007/BF00303439
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DOI: https://doi.org/10.1007/BF00303439