Skip to main content
SpringerLink
Log in

Genomic distribution of copia-like transposable elements in somatic tissues and during development of Drosophila melanogaster

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

The genomic distribution of elements of the copia, 412, B 104, mdg 1, mdg 4 and 1731 transposon families was compared by the Southern technique in DNA preparations extracted from brains, salivary glands and adult flies of two related Drosophila lines. The copia, 412 and mdg 1 sequences were also probed in DNA from sperm, embryos, and 1st and 2nd instar larvae. The homogeneity of the patterns observed shows that somatic transposition is unlikely to occur frequently. A correlation between mobility and the euchromatic or heterochromatic location of transposable elements is discussed. In addition, an explanation of the variable band intensities of transposable elements in Southern autoradiographs is proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ananiev EV, Ilyin YV (1981) A comparative study of the location of mobile dispersed genes in salivary gland and midgut polytene chromosomes of Drosophila melanogaster. Chromosoma 82:429–435

    Google Scholar 

  • Ananiev EV, Gvozdey VA, Ilyin YV, Tchurikov NA, Georgiev GP (1978) Reiterated genes with varing location in intercalary heterochromatin of Drosophila melanogaster polytene chromosomes. Chromosoma 70:1–17

    Google Scholar 

  • Ananiev EV, Barsky VE, Ilyin YV, Ryzic MV (1984) The arrangement of transposable elements in the polytene chromosomes of Drosophila melanogaster. Chromosoma 90:366–377

    Google Scholar 

  • Bayev Jr AA, Lyubomirskaya NV, Dzhumagalier EB, Ananiev EV, Amiantova IG, Ilyin YV (1984) Structural organization of transposable element mdg 4 from Drosophila melanogaster and a nucleotide sequence of its long terminal repeats. Nucleic Acids Res 12:3707–3723

    Google Scholar 

  • Biémont C, Aouar A (1987) Copy number dependent transpositions and exisions of the mdg 1 mobile element in inbred lines of Drosophila melanogaster. Heredity 58:39–47

    Google Scholar 

  • Biémont C, Aouar A, Arnault C (1987) Genome reshuffling of the copia element in an inbred line of Drosophila melanogaster. Nature 329:742–744

    Google Scholar 

  • Bonaldo MF, Santelli RV, Lara FJS (1979) The transcript from a DNA puff of Rhynosciara and its migration to the cytoplasm. Cell 17:827–833

    Google Scholar 

  • Bryan GJ, Jacobson JW, Hartl DL (1987) Heritable somatic excision of a Drosophila transposon. Science 235:1636–1638

    Google Scholar 

  • Couderec JL, Sobrier ML, Giraud G, Becker JL, Dasitugue B (1983) Actin gene expression is modulated by Ectysterone in a Drosophila cell line. J Mol Biol 164:419–430

    Google Scholar 

  • Crozatier M, Vaury C, Busseau I, Pelisson A, Bucheton A (1988) Structure and genomic organization of I elements involved in I-R hybrid dysgenesis in Drosophila melanogaster. Nucleic Acids Res 16:9199–9213

    Google Scholar 

  • Dickson E, Boyd JB, Laird CD (1971) Sequence diversity of polytene chromosome DNA from Drosophila hydei. J Mol Biol 61:615–627

    Google Scholar 

  • Di Nocera PP, Dawid IB (1983) Interdigitated arrangement of two oligo(A)-terminated DNA sequences in Drosophila. Nucleic Acids Res 11:5475–5482

    Google Scholar 

  • Di Nocera PP, Graziani F, Lavorgna G (1986) Genomic and structural organization of Drosophila melanogaster. G elements. Nucleic Acids Res 14:675–691

    Google Scholar 

  • Dolfini S (1971) Karyotype polymorphism in a cell population of Drosophila melanogaster culture in vitro. Chromosoma 33:196–208

    Google Scholar 

  • Echalier G, Ohanessian A (1969) Isolementen cultures in vitro, de lignees cellulaires diploides de Drosophila melanogaster. CR Acad Sci Paris 268:1771–1773

    Google Scholar 

  • Emmons SW, Yesner L (1984) High-frequency excision of transposable element Tc1 in the Nematode Caenorhabditis elegans is limited to somatic cells. Cell 36:599–609

    Google Scholar 

  • Finnegan DJ (1985) Transposable elements in eukaryotes. Int Rev Cytol 93:281–326

    Google Scholar 

  • Finnegan DJ, Fawcett DA (1986) Transposable elements in Drosophila melanogaster. In: Oxford surveys of eukaryotic genes, vol 3, pp 24–62

  • Finnegan DJ, Rubin GM, Young MW, Hogness DS (1978) Repeated gene families in Drosophila melanogaster. Cold Spring Harbor Symp Quant Biol 42:1053–1063

    Google Scholar 

  • Gall JG, Cohen EH, Polan ML (1971) Repetitive DNA sequences in Drosophila. Chromosoma 33:319–344

    Google Scholar 

  • Green MM (1988) Mobile DNA elements and spontaneous gene mutation. In: Lambert ME, McDonald JF, Weinstein IB (eds) Eucaryotic transposable elements as mutagenic agents. 30th Banbury report, Cold Spring Harbor Laboratory, pp 41–50

  • Hennig W (1967) Untersuchungen zur Struktur und Funktion des Lampenbursten-Y-Chromosoms in der Spermatogenese von Drosophila. Chromosoma 22:294–357

    Google Scholar 

  • Holden JJ, Ashburner M (1978) Patterns of puffing activity in the salivary gland chromosomes of Drosophila. Chromosoma 68:2051–2055

    Google Scholar 

  • Ilyin YV, Tchurikov NA, Ananiev EV, Ryskov AP, Yenikopolov GN, Limborska SA, Maleeva NE, Gvozdev VA, Georgiev GP (1978) Studies on the DNA fragments of mammals and Drosophila containing structural genes and adjacent sequences. Cold Spring Harbor Symp Quant Biol 42:959–969

    Google Scholar 

  • Ilyin YV, Chmellauskaite VG, Ananlev EV, Lyubomirskaya NV, Kulguskin VV, Bayev Jr AA, Georgiev GP (1980) Mobile dispersed genetic element mdg 1 of Drosophila melanogaster: structural organization. Nucleic Acids Res 8:5333–5346

    Google Scholar 

  • Junakovic N, Angelucci V (1986) Polymorphisms in the genomic distribution of copia-like elements in related laboratory stocks of Drosophila melanogaster. J Mol Evol 24:83–88

    Google Scholar 

  • Junakovic N, Caneva R, Ballario P (1984) Genomic distribution of copia-like elements in laboratory stocks of Drosophila melanogaster. Chromosoma 90:378–382

    Google Scholar 

  • Junakovic N, Di Franco C, Barsanti P, Palumbo G (1986) Transposition of copia-like nomadic elements can be induced by heatshock. J Mol Evol 24:89–92

    Google Scholar 

  • Junakovic N, Di Franco C, Best-Belpomme M, Echalier G (1988) On the transposition of copia-like nomadic elements in cultured cells of Drosophila melanogaster. Chromosoma 98:212–218

    Google Scholar 

  • Laski FA, Rio DC, Rubin GM (1986) Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing. Cell 44:7–19

    Google Scholar 

  • Levine M, Garen A, Lepesant JA, Lepesant-Kejzlarova J (1981) Constancy of somatic DNA organization in developmentally regulated regions of the Drosophila genome. Proc Natl Sci USA 78:2417–2421

    Google Scholar 

  • Loukas M, Kafatos CF (1986) The actin loci in the genus Drosophila: Establishment of chromosomal homologies among distantly related species by in situ hybridization. Chromosoma 94:297–308

    Google Scholar 

  • Miklos GLG, Yamamoto M, Davies J, Pirrotta V (1988) Microcloning reveals a high frequency of repetitive sequences characteristic of chromosome 4 and the beta-heterochromatin of Drosophila melanogaster. Proc Natl Acad Sci USA 85:2051–2055

    Google Scholar 

  • Peronnet F, Becker LL, Becker J, d'Auriol L, Galibert F, Best-Belpomme M (1986) 1731, a new retrotransposon with hormone modulated expression. Nucleic Acids Res 14:9017–9033

    Google Scholar 

  • Pierce DA, Luchesi JC (1981) Analysis of a dispersed repetitive DNA sequence in isogenic lines of Drosophila melanogaster. Chromosoma 82:471–492

    Google Scholar 

  • Piñol J, Francino O, Fontdevila A, Cabré O (1988) Rapid isolation of Drosophila high molecular weight DNA to obtain genomic libraries. Nucleic Acids Res 16:2736

    Google Scholar 

  • Potter SS, Thomas Jr CA (1978) The two-dimensional fractionation of Drosophila DNA. Cold Spring Harbor Symp Quant Biol 42:1023–1031

    Google Scholar 

  • Potter SS, Brorein Jr WJ, Dunsmuir P, Rubin GM (1979) Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell 17:415–427

    Google Scholar 

  • Richards G (1980) The polytene chromosomes in the fat body nuclei of Drosophila melanogaster. Chromosoma 79:241–250

    Google Scholar 

  • Rubin GM (1983) In: Shapiro JA (ed) Mobile genetic elements. Academic Press, New York, pp 329–361

    Google Scholar 

  • Scherer G, Tschudi C, Perera J, Delius H, Pirrotta V (1982) B 104, a new dispersed repeated gene family in Drosophila melanogaster and its analogies with retroviruses. J Mol Biol 157:435–451

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Spradling AC, Mahowald AP (1980) Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster. Proc Natl Acad Sci USA 77:1096–1100

    Google Scholar 

  • Spradling AC, Rubin MG (1981) Drosophila genome organization: conserved and dynamic aspects. Annu Rev Genet 15:219–264

    Google Scholar 

  • Strobel E, Dunsmuir P, Rubin GM (1979) Polymorphisms in chromosomal location of elements of the 412, copia and 297 dispersed repeated gene families. Cell 17:429–439

    Google Scholar 

  • Tchurikov NA, Zelentsova ES, Georgiev GP (1980) Clusters containing different mobile dispersed genes in the genome of Drosophila melanogaster. Nucleic Acids Res 8:1243–1258

    Google Scholar 

  • Tchurikov NA, Ilyin YV, Skryabin KG, Ananiev EV, Bayev Jr AA, Krayev AS, Zelentsova ES, Kulguskin VV, Lyubomirskaya NV, Georgiev GP (1981) General properties of mobile dispersed genetic elements in Drosophila melanogaster. Cold Spring Harbor Symp Quant Biol 45:655–665

    Google Scholar 

  • Vaury C, Bucheton A, Pélisson A (1989) The β heterochromatic sequences flanking the I elements are themselves defective transposable elements. Chromosoma 98:215–224

    Google Scholar 

  • Wensink PC, Tabata D, Pachl C (1979) The clustered and scrambled arrangement of moderately repetitive elements in Drosophila DNA. Cell 18:1231–1246

    Google Scholar 

  • Young MW, Schwartz HE (1981) Nomadic gene families in Drosophila. Cold Spring Harbor Symp Quant Biol 45:629–640

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centro per lo Studio degli Acidi Nucleici C.N.R., Università “La Sapienza”, Piazzale A. Moro 5, I-00185, Roma, Italy

    Carmen Di Franco & Nikolaj Junakovic

  2. Centro di Genetica Evoluzionistica C.N.R., Università “La Sapienza”, Piazzale A. Moro 5, I-00185, Roma, Italy

    Claudio Pisano

  3. Dipartimento di Genetica e Biologia Molecolare, Università “La Sapienza”, Piazzale A. Moro 5, I-00185, Roma, Italy

    Patrizio Dimitri

  4. International Institute of Genetics and Biophysics, via Marconi 12, Napoli, Italy

    Silvia Gigliotti

Authors
  1. Carmen Di Franco
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Claudio Pisano
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Patrizio Dimitri
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Silvia Gigliotti
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nikolaj Junakovic
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Di Franco, C., Pisano, C., Dimitri, P. et al. Genomic distribution of copia-like transposable elements in somatic tissues and during development of Drosophila melanogaster . Chromosoma 98, 402–410 (1989). https://doi.org/10.1007/BF00292785

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00292785

Keywords

Search

Navigation