Abstract
Immunoscintigraphy was performed in 25 patients with malignant melanoma using a 99mTc labelled monoclonal antibody (225.28S). In four cases, imaging was repeated following treatment with recombinant gamma interferon. In 11 cases, tissue samples of metastatic lesions were investigated for the expression of melanoma associated antigens by immunohistochemical techniques. Overall, 59% of known lesions were detected by imaging. The highest rates of detection were in bone, liver and lymph nodes and the lowest in lung, stomach and bowel. Six lesions were detected which had not shown up on routine clinical and radiological examination. There was no evidence of enhanced or induced HMW-MAA expression following interferon treatment. This technique has been found to be useful in staging patients and has been free from side effects.
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Balch CM, Song SJ, Shaw MM, Milton GW (1985) An analysis of prognostic factors in 4000 patients with cutaneous melanoma. In: Balch CM, Milton GW, Shaw MM, Soong SJ (eds) Cutaneous Melanoma Clinical Management and Treatment Results Worldwide. JB Lippincott, Philadelphia, pp 321–352
Buraggi GL, Callegaro L, Turrin A, Cascinelli N, Attili A, Emanuelli H, Gasparini M, Deleide G, Plassio G, Dovis M, Mariani G, Natali PG, Scassellati GA, Rosa U, Ferrone S (1984) Immunoscintigraphy with 123I, 99mTc and 111In labelled F(ab′) 2 fragments of monoclonal antibodies to a human high molecular weight-melanoma associated antigen. J Nucl Med Allied Sci 28:283–295
Buraggi GL, Callegaro L, Mariani G, Turrin A, Cascinelli N, Attili A, Bombardieri E, Terno G, Plassio G, Dovis M, Mazzuca N, Natali PG, Scassellati GA, Rosa U, Ferrone S (1985) Imaging with 131I labelled monoclonal antibodies to a high molecular weight melanoma-associated antigen in patients with melanoma: Efficacy of whole immunoglobulin and its F(ab′) 2 fragments. Cancer Res 45:3378–3387
Cerny T, Ghosh AK, Street AJ, Wagstaff J, Thatcher N, Harns M, Moore M (1986) Expression of MHC products and tumour associated antigens (TAA) before and after treatment of malignant melanoma with IFN-. Br J Cancer 54:146
Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, Macdonald S, Pulford KAS, Stein H, Mason DY (1986) Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219–229
Giacomini P, Veglia F, Cordiali Fei P, Rehie T, Natali PG, Ferrone S (1984) Level of a membrane-bound high-molecular weight melanoma-associated antigen and a cytoplasmic melanoma-associated antigen in surgically removed tissues and in sera from patients with melanoma. Cancer Res 44:1281–1287
Imai K, Wilson BS, Kay NE, Ferrone A (1981) Monoclonal antibodies to human melanoma cells: Comparison of serological results of several laboratories and molecular prolife of melanoma associated antigens. In: Hammerling GJ, Hammerling U, Kearney JF (eds) Monoclonal antibodies and T cell hybrids. Elsevier, New York, Amsterdam, p 183
Riva P, Paganelli G, Landi G, Amadori D, Tison V, Marangolo M (1985) False negative and false positive results in immunoscintigraphy of malignant melanoma with 225.28S-F(ab′) 2 labelled with 99mTc or 111In. J Nucl Med Allied Sci 29:141 (Abstr)
Scheidhauer K, Stefani F, Mark LA, Schumacher U, Moser E (1986) Primary ocular melanoma: Evaluation with radioimunoscintigraphy. Br J Cancer 54:540 (Abstr)
Siccardi AG, Buraggi GL, Collegaro L, Mariani G, Natali PG, Abbati A, Bestagno M, Caputo V, Mansi L, Masi R, Paganelli G, Riva P, Salvatore M, Sanguineti M, Troncone L, Turio GL, Scassellati GA, Ferrone S (1986) Multicentre study of immunoscintigraphy with radiolabelled monoclonal antibodies in patients with melanoma. Cancer Res 46:4817–4822
Wilson BS, Imai K, Natali PG, Ferrone S (1981) Distribution and molecular characterisation of a cell-surface and a cytoplasmic antigen detectable in human melanoma cells with monoclonal antibodies. Int J Cancer 28:293–300
Wilson BS, Giacomini P, Kohzoh I, Natali PG, Nakanishi T, Ruberto G, Ferrone S (1982) Human melanoma associated antigens identified with monoclonal antibodies (structural profile and potential usefulness in immunodiagnosis and immunotherapy). La Ricerca in Clinica ein Laboratorio XII:517–538
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Cerny, T., Owens, S.E., Mackenzie, S.A. et al. Immunoscintigraphy with 99mTc labelled F(ab′)2 fragments of an anti melanoma monoclonal antibody (225.28S) in patients with metastatic malignant melanoma. Eur J Nucl Med 13, 130–133 (1987). https://doi.org/10.1007/BF00289024
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DOI: https://doi.org/10.1007/BF00289024