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Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus)

I. Morphology of the autosomal complement in spread preparations

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Abstract

Using the Counce-Meyer spreading technique, in over 70 spermatocytes it was possible consistently to obtain whole, flattened nuclei containing complete sets of pachytene SCs. The SCs are visible in both the phase and electron microscopes. Each SC is morphologically intact, preferentially stained, and attached to the nuclear envelope by a dense, terminal plaque. It is thus possible to trace each SC for its entire length. Also, a structure representing the kinetochore is clearly visible in each autosomal SC. Karyotypes comparable to the somatic karyotype can be constructed by arranging SCs according to length and kinetochore position. The observed regularity of SC morphology implies structural stability sufficient to withstand the stresses imposed by the procedure.— A coarse network of closely packed nuclear annuli connecting SC attachment plaques often provides end-to-end associations and may tend to immobilize SCs during processing.— Three kinds of perturbation of SC structure are encountered. Twists in the SC frequently occur, but no regular pattern or correspondence with chiasma distribution is observed. SCs occasionally hook around each other without disruption, but in two instances the unpaired axis of the X apparently was interlocked within an autosomal SC. Stretching of the SC is infrequent; it is conspicuous when it occurs and is usually associated with other obvious distortions of the nucleus.— Distinctive morphologies of the X and Y chromosomes facilitate their identification in all preparations. — During zygotene, autosomal synapsis, i.e., the formation of SCs from the pairing of single axial elements, initiates at distal ends and terminates at the kinetochore region; neither initiation nor termination is synchronous among all autosomes.

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References

  • Aaronson, R.B., Blöbel, G.: Isolation of nuclear pore complexes in association with a lamina. Proc. nat. Acad. Sci. (Wash.) 72, 1007–1011 (1975)

    Google Scholar 

  • Ashley, T., Wagenaar, E.B.: Telomeric associations of gametic and somatic chromosomes in diploid and autotetraploid Ornithogalum virens. Canad. J. Genet. Cytol. 16, 61–76 (1974)

    Google Scholar 

  • Baker, T.G., Franchi, L.L.: The structure of the chromosome in human primordial oocytes. Chromosoma (Berl.) 22, 358–377 (1967)

    Google Scholar 

  • Brown, W.V., Stack, S.M.: Somatic pairing as a regular preliminary to meiosis. Bull. Torrey Bot. Club 95, 369–378 (1968)

    Google Scholar 

  • Burdick, A.B.: Somatic cell chromosome interconnections in trypan preparations of Chinese hamster testicular cells. Exp. Cell Res. 99, 425–428 (1976)

    Google Scholar 

  • Byers, B., Goetsch, L.: Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.) 72, 5056–5060 (1975)

    Google Scholar 

  • Carpenter, A.T.C.: Electron microscopy of meiosis in Drosophila melanogaster females. I. Structure, arrangement, and temporal change of the synaptonemal complex in wild-type. Chromosoma (Berl.) 51, 157–182 (1975)

    Google Scholar 

  • Comings, D.E., Okada, T.A.: Whole mount electron microscopy of meiotic chromosomes and the synaptonemal complex. Chromosoma (Berl.) 30, 269–286 (1970)

    Google Scholar 

  • Comings, D.E., Okada, T.A.: Mechanisms of chromosome banding. VI Whole mount electron microscopy of banded metaphase chromosomes and a comparison with pachytene chromosomes. Exp. Cell Res. 93, 267–274 (1975)

    Google Scholar 

  • Costello, D.P.: Identical linear order of chromosomes in both gametes of the acoel turbellarian Polychoerus carmelensis: a preliminary note. Proc. nat. Acad. Sci. (Wash.) 67, 1951–1958 (1970)

    Google Scholar 

  • Counce, S.J., Meyer, G.F.: Differentiation of the synaptonemal complex and the kinetochore in Locusta spermatocytes studied by whole mount electron microscopy. Chromosoma (Berl.) 44, 231–253 (1973)

    Google Scholar 

  • Fawcett, D.W.: On the occurrence of a fibrous lamina on the inner aspect of the nuclear envelope in certain cells of vertebrates. Amer. J. Anat. 119, 129–146 (1966)

    Google Scholar 

  • Franke, W.W.: Nuclear envelopes. Structure and biochemistry of the nuclear envelope. Phil. Trans. roy. Soc. London, B 268, 67–93 (1974)

    Google Scholar 

  • Fredga, K., Santesson, B.: Male meiosis in the Syrian, Chinese and European hamsters. Hereditas (Lund) 52, 36–48 (1964)

    Google Scholar 

  • Gillies, C.B.: Reconstruction of the Neurospora crassa pachytene karyotype from serial sections of synaptonemal complexes. Chromosoma (Berl.) 36, 119–130 (1972)

    Google Scholar 

  • Gillies, C.B.: Ultrastructural analysis of Maize pachytene karyotypes by three dimensional reconstruction of the synaptonemal complexes. Chromosoma (Berl.) 43, 145–176 (1973)

    Google Scholar 

  • Gillies, C.B.: Synaptonemal complex and chromosome structure. Ann. Rev. Genet. 9, 91–109 (1975a)

    Google Scholar 

  • Gillies, C.B.: An ultrastructural analysis of chromosome pairing in Maize. C. R. Trav. Lab. Carlsberg 40, 135–161 (1975a)

    Google Scholar 

  • Hsu, T.C., Arrighi, F.E.: Distribution of constitutive heterochromatin in mammalian chromosomes. Chromosoma (Berl.) 34, 243–253 (1971)

    Google Scholar 

  • Hsu, T.C., Zenzes, M.T.: Mammalian chromosomes in vitro. XVII. Idiogram of the Chinese hamster. J. nat. Cancer Inst. 34, 857–869 (1964)

    Google Scholar 

  • Hungerford, D.A.: Chromosome structure and function in man. I. Pachytene mapping in the male, improved methods and general discussion of initial results. Cytogenetics 10, 23–32 (1971)

    Google Scholar 

  • LaCour, L.F., Wells, B.: Nuclear pores at prophase of meiosis in plants. Phil. Trans. roy. Soc. London, B 268, 95–100 (1974)

    Google Scholar 

  • Luciani, J.M., Morazzani, M.-R., Stahl, A.: Identification of pachytene bivalents in human male meiosis using G-banding technique. Chromosoma (Berl.) 52, 275–282 (1975)

    Google Scholar 

  • McKay, R.D.G.: The mechanism of G and C banding in mammalian metaphase chromosomes. Chromosoma (Berl.) 44, 1–14 (1973)

    Google Scholar 

  • Miller, O.: Visualization of nuclear genes. Science 164, 955–957 (1969)

    Google Scholar 

  • Moens, P.B.: The fine structure of meiotic chromosome polarization and pairing in Locusta migratoria spermatocytes. Chromosoma (Berl.) 28, 1–25 (1969)

    Google Scholar 

  • Moens, P.B.: Fine structure of chromosome coiling at meiotic prophase in Rhoeo discolor. Canad. J. Genet. Cytol. 14, 801–808 (1972)

    Google Scholar 

  • Moens, P.B.: Quantitative electron microscopy of chromosome organization at meiotic prophase. Cold Spr. Harb. Symp. quant. Biol. 38, 99–107 (1973)

    Google Scholar 

  • Moens, P.B., Perkins, F.O.: Chromosome number of a small protist. Science 166, 1289–1291 (1969)

    Google Scholar 

  • Moses, M.J.: Synaptinemal complex. Ann. Rev. Genet. 2, 363–412 (1968)

    Google Scholar 

  • Moses, M. J.: Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus). II. Morphology of the XY pair in spread preparations. Chromosoma (Berl.) 60, 127–137 (1977)

    Google Scholar 

  • Moses, M.J., Counce, S.J.: Synaptonemal complex karyotyping in spreads of mammalian spermatocytes. In: Mechanisms in recombination (R.F. Grell, ed.), pp. 385–390. New York: Plenum Publ. Corp. 1974

    Google Scholar 

  • Moses, M.J., Counce, S.J.: Synaptonemal complex karyotyping in spreads of mammalian spermatocytes. J. Cell Biol. 63, 233a (1974b)

  • Moses, M.J., Counce, S.J.: Analysis of synaptonemal complex karyotypes in five mammals. J. Cell Biol. 70, 131a (1976)

  • Moses, M.J., Counce, S.J., Hamilton, A.E., Karatsis, P.A., Russell, L.B.: The synaptonemal complex as a tool in cytogenetic studies. Genetics 80, s58 (1975)

    Google Scholar 

  • Moses, M.J., Counce, S.J., Paulson, D.F.: Synaptonemal complex complement of man in spreads of spermatocytes, with details of the sex chromosome pair. Science 187, 363–365 (1975)

    Google Scholar 

  • Moses, M.J., Slatton, G., Gambling, T., Starmer, C.F.: Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus). III. Quantitative evaluation. Chromosoma (Berl.) (in press, 1977)

  • Moses, M. J., Solari, A. J.: Positive contrast staining and protected drying of surface spreads: electron microscopy of the synaptonemal complex by a new method. J. Ultrastruct. Res. 54, 109–114 (1976)

    Google Scholar 

  • Pathak, S., Hsu, T.C., Markwong, A.: Pachytene mapping of the male Chinese hamster. Cytogenet. Cell Genet. 17, 1–8 (1976)

    Google Scholar 

  • Rasmussen, S.W.: The meiotic prophase in Bombyx mori females analyzed by three-dimensional reconstructions of synaptonemal complexes. Chromosoma (Berl.) 54, 245–293 (1976)

    Google Scholar 

  • Solari, A.J.: Changes in the sex chromosomes during meiotic prophase in mouse spermatocytes. Genetics 61, Suppl., 113–120 (1969)

    Google Scholar 

  • Solari, A.J.: The spatial relationship of the X and Y chromosomes during meiotic prophase in mouse spermatocytes. Chromosoma (Berl.) 29, 217–236 (1970)

    Google Scholar 

  • Solari, A.J.: Ultrastructure and composition of the synaptonemal complex in spread and negatively stained spermatocytes of the golden hamster and the albino rat. Chromosoma (Berl.) 39, 237–263 (1972)

    Google Scholar 

  • Solari, A.J.: The behavior of the XY pair in mammals. Int. Rev. Cytol. 38, 273–317 (1974)

    Google Scholar 

  • Solari, A.J., Moses, M.J.: The structure of the central region in the synaptonemal complexes of hamster and cricket spermatocytes. J. Cell Biol. 56, 145–152 (1973)

    Google Scholar 

  • Stern, H., Hotta, Y.: Biochemical controls of meiosis. Ann. Rev. Genet. 7, 37–66 (1973)

    Google Scholar 

  • Wagenaar, E.B.: End-to-end attachments in mitotic interphase and their possible significance in meiotic chromosome pairing. Chromosoma (Berl.) 26, 410–426 (1969)

    Google Scholar 

  • Westergaard, M., Wettstein, D. von: The synaptinemal complex. Ann. Rev. Genet. 6, 71–110 (1972)

    Google Scholar 

  • Wettstein, R., Sotelo, J.R.: Electron microscope serial reconstruction of the spermatocytes I nuclei at pachytene. J. Microscopie 6, 557–576 (1967)

    Google Scholar 

  • Woollam, D.H., Ford, E.M.: The fine structure of the mammalian chromosome in meiotic prophase with special reference to the synaptinemal complex. J. Anat. (Lond.) 98, 163–173 (1964)

    Google Scholar 

  • Woollam, D.H., Ford, E.H., Millen, J.: The attachment of pachytene chromosomes to the nuclear membrane in mammalian spermatocytes. Exp. Cell Res. 42, 657–661 (1966)

    Google Scholar 

Download references

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  1. Department of Anatomy, Duke University Medical Center, 27710, Durham, North Carolina, USA

    Montrose J. Moses

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Moses, M.J. Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus). Chromosoma 60, 99–125 (1977). https://doi.org/10.1007/BF00288459

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  • DOI: https://doi.org/10.1007/BF00288459

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