Skip to main content
SpringerLink
Log in

On the specificity of histochemically demonstrable bile canalicular phosphatase activities

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

Bile canalicular phosphatase activity in frog, chicken, rat and cat has been studied with respect to substrate specificity, pH optimum and effect of various stimulators and inhibitors.

It is concluded that three different bile canalicular phosphatase activities may be demonstrated histochemically: 1. A strong non-specific nucleoside diphosphatase able to split ATP and most diphosphates. Its relevance is, however, uncertain and requires confirmation by biochemical studies. 2. A non-specific alkaline phosphatase, 3. 5′-nucleotidase.

Evidence is presented that the bile oanalicular staining reflects real enzyme activity and not artificial non-enzymatic hydrolysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abel, J.H.: Electron microscopic demonstration of adenosinetriphosphate phosphohydrolase activity in herring gull salt glands. J. Histochem. Cytochem. 17, 570–584 (1966).

    Google Scholar 

  • Coleman, J.R.: Biochemical and cytochemical demonstration of ATP-ase activity in nuclei. J. Cell Biol. 27, 20A (1965).

    Google Scholar 

  • Essner, E., Novikoff, A.B., Masek, B.: Adenosinetriphosphatase and 5-nucleotidase activities in the plasma membrane of liver cells as revealed by electron microscopy. J. biophys. biochem. Cytol. 4, 711–716 (1958).

    Google Scholar 

  • Freiman, D.G., Kaplan, N.: Studies on the histochemical differentiation of enzymes hydrolyzing adenosine triphosphate. J. Histochem. Cytochem. 8, 159–170 (1960).

    Google Scholar 

  • Gomori, G.: Distribution of phosphatase in normal organs and tissues. J. cell. comp. Physiol. 17, 71–83 (1941).

    Google Scholar 

  • —: Microscopic histochemistry. Chicago: Chicago Univ. Press 1952.

    Google Scholar 

  • Jacobsen, N.O., Jörgensen, P.L.: A quantitative biochemical and histochemical study of the lead method for localization of adenosine triphosphate-hydrolyzing enzymes. J. Histochem. Cytochem. 17, 443–453 (1969).

    Google Scholar 

  • Kornberg, A.: Reversible enzymatic synthesis of diphosphopyridine nucleotide and inorganic pyrophosphate. J. biol. Chem. 182, 779–793 (1950).

    Google Scholar 

  • Marchesi, V.T., Palade, G.T.: The localization of Mg-Na-K-activated adenosinetriphosphatase on red cell ghost membranes. J. Cell Biol. 35, 385–404 (1967).

    Google Scholar 

  • Mieler, W., Zoellner, H.: Der histochemische Nachweis von Phosphatasen in Leber und Niere mit verschiedenen Substraten. Acta histochem. (Jena) 8, 325–339 (1959).

    Google Scholar 

  • Moses, H.L., Rosenthal, A.L.: Pitfalls in the use of lead ion for histochemical localization of nucleoside phosphatases. J. Histochem. Cytochem. 16, 530–539 (1968).

    Google Scholar 

  • Novikoff, A.B.: Enzyme localization with Wachstein-Meisel procedures: Real or artifact. J. Histochem. Cytochem. 15, 353–354 (1967).

    Google Scholar 

  • —, Drucker, J., Shin, W.Y., Goldfischer, S.: Further studies on the apparent adenosinetriphosphatase activity of cell membranes in formol-calcium-fixed tissues. J. Histochem. Cytochem. 9, 431–451 (1961).

    Google Scholar 

  • —, Hausman, D.H., Podber, E.: The localization of adenosinetriphosphatase in liver. In situ stained and cell fractionation studies. J. Histochem. Cytochem. 6, 61–71 (1958).

    Google Scholar 

  • —, Hecht, L., Podber, E., Ryan, J.: Phosphates of rat liver. I. The dephosphorylation of adenosinetriphosphate. J. biol. Chem. 194, 153–170 (1952).

    Google Scholar 

  • Padykula, H.A., Herman, E.: The specificity of the histochemical method for adenosine triphosphatase. J. Histochem. Cytochem. 3, 170–183 (1955).

    Google Scholar 

  • Rosenthal, A. S., Moses, H.L., Tice, L., Ganote, C.E.: Lead ion and phosphatase histochemistry. III. The effects of lead and adenosine triphosphate concentration on the incorporation of phosphate into fixed tissue. J. Histochem. Cytochem. 17, 607–612 (1969).

    Google Scholar 

  • Sandström, B.: Liver fixation for electron microscopy by means of transparenchymal perfusion with glutaraldehyde. Lab. Invest. (1970) (to be published).

  • —, Westman, J.: Non-freezing light and electron microscopic histochemistry by means of polyetyleneglycol embedding. Histochemie 19, 181–183 (1969).

    Google Scholar 

  • Vorbrodt, A.: Histochemical studies on thiaminepyrophosphatase localization in the adult, embryonic and neoplastic liver cells using light and electron microscopy. Acta histochem. (Jena) 28, 204–214 (1967).

    Google Scholar 

  • Wachstein, M., Meisel, E.: Histochemistry of hepatic phosphatases at a physiological pH. Amer. J. clin. Path. 27, 13–23 (1957).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Human Anatomy, University of Uppsala, Uppsala, Sweden

    Björn Sandström

Authors
  1. Björn Sandström
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This study was supported in part by a grant from the Swedish Medical Research Council.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sandström, B. On the specificity of histochemically demonstrable bile canalicular phosphatase activities. Histochemie 22, 316–323 (1970). https://doi.org/10.1007/BF00277459

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00277459

Keywords

Search

Navigation