Abstract
Vanadyl sulphate (10–500 mg/l), when added to cell suspension cultures of Catharanthus roseus stimulated increased intracellular accumulation of catharanthine and ajmalicine. This response was demonstrated in both flask and fermenter (30 litre) systems. The response varied, and depended upon cell line, concentration of vanadyl sulphate and the stage of the growth phase at which the cells were treated. This process has the potential to increase the yield and reduce the production time for commercially useful secondary plant metabolites.
Similar content being viewed by others
Abbreviations
- Ajm:
-
ajmalicine
- Cath:
-
catharanthine
- CAS:
-
ceric ammonium sulphate
- VOSO4 :
-
vanadyl sulphate
- FW:
-
fresh weight
- n.d.:
-
not detected
References
Arfmann HA, Kohl W, Wray V (1985) Z Naturforsch 40 c:21–25.
Ayabe S, Iida K, Furuya T (1986) Plant Cell Reports 3:186–189.
DiCosmo F, Towers GHN (1984) Recent Adv in Phytochem 18:97–175.
DiCosmo F, Misawa M (1985) TIBTECH 3:318–322.
DiCosmo F, Quesnel A, Misawa M, Tallevi SG (In press). Appl Biochem and Biotech
Eilert U, Ehmke A, Wolters B (1984) Planta Medica:508–512.
Eilert U, Kurz WGW, Constabel, F (1985) J Plant Physiol 119:65–76.
Farnsworth NR, Blomster RN, Damratoski D, Meer WA, Cammarato LV (1964) Lloydia 27:302–314.
Fowler MW, (1983) In: Mantell SH, Smith H (eds), Plant Biotechnology Cambridge University Press pp 3–8.
Hattori T, Ohta Y (1985) Plant Cell Physiol 26:1101–1110.
Lee S-L, Cheng K-D, Scott AI (1981) Phytochèm 20:1841–1843.
Macara IG (1980) TIBS 5:92–94.
Morris P (1986) Planta Medica:121–126.
Murashige TO, Skoog F (1962) Physiol Plant 15:473–497.
Rokem JS, Tal B, Goldberg I (1985) J Nat Prods 48:210–222.
Schenk RU, Hildebrandt AC (1972) Can J Bot 50:199–204
Smith, JI, Smart NJ, Quesnel AA, Misawa M, Kurz W (1986). In: Somers DA, Gengenbach BG, Biesboer DD, Hackett WP, Green CE, (eds) Abstracts VI International Congress of Plant Tissue and Cell Culture p 248.
Sze H (1984) Physiol Plant 61:683–691.
Wilson SB, King PJ, Street HE (1971) J Exp Bot 22:177–207
Author information
Authors and Affiliations
Additional information
Communicated by F. Constabel
Rights and permissions
About this article
Cite this article
Smith, J.I., Smart, N.J., Misawa, M. et al. Increased accumulation of indole alkaloids by some cell lines of Catharanthus roseus in response to addition of vanadyl sulphate. Plant Cell Reports 6, 142–145 (1987). https://doi.org/10.1007/BF00276673
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00276673