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Callus formation from mesophyll protoplasts of Fagus sylvatica L.

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Abstract

Viable protoplasts of European beech (Fagus sylvatica L.) were isolated from sterilized young leaves of juvenile (3–5 years) and mature (40 years) trees. Isolation in a saline solution containing 0.5% (w/v) Pectinol and 2% (w/v) Cellulase R-10 yielded 3×107 protoplasts per gram fresh weight. Protoplast culture in modified Kao and Michayluk (1975) medium resulted in cell wall regeneration and sustained cell divisions with the formation of colonies and microcalli.

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Abbreviations

KM:

Kao and Michayluk (1975)

LS:

Linsmaier and Skoog (1965)

MES:

2(N-morpholino)-ethane-sulfonic acid

NAA:

1-naphthalene acetic acid

PVP:

Polyvinylpyrrolidone

Tween 80:

Polyoxy-ethylene-sorbitan-monooleate

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Authors and Affiliations

  1. Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstrasse 70, D-6000, Frankfurt am Main, Germany

    Hermann Lang & Hans Willy Kohlenbach

Authors
  1. Hermann Lang
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  2. Hans Willy Kohlenbach
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Additional information

Communicated by I. Potrykus

Dedicated to Landesforstmeister Prof. Dr. Hans-Joachim Fröhlich on the occasion of his 65th birthday.

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Lang, H., Kohlenbach, H.W. Callus formation from mesophyll protoplasts of Fagus sylvatica L.. Plant Cell Reports 7, 485–488 (1988). https://doi.org/10.1007/BF00272737

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  • DOI: https://doi.org/10.1007/BF00272737

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