Summary
The mapping of three genes (speA, speB and metK) involved in the biosynthesis of spermidine is described. They are located between minutes 56 and 57 on the linkage map of Escherichia coli. The clustering of these genes suggests the possibility of an operon for spermidine synthesis. Two other genes, argP, controlling the production of an arginine permease, and can, conferring resistance to canavanine, were found to be located in the same region.
Similar content being viewed by others
References
Clark, A. J.: Genetic analysis of a “double-male” strain of Escherichia coli K-12. Genetics 48, 105–120 (1963).
Cooper, P. H., Hirshfield, I. N., Maas, W. K.: Map location of arginyl-tRNA synthetase mutations in Escherichia coli K-12. Molec. gen. Genet. 104, 383–390 (1969).
Glansdorff, N.: Topography of cotransducible arginine mutations in Escherichia coli K-12. Genetics 51, 167–179 (1965).
Greene, R. C., Su, C. H., Holloway, C. T.: S-adenosylmethionine synthetase deficient mutants of Escherichia coli K-12 with impaired control of methionine biosynthesis. Biochem. biophys. Res. Commun. 38, 1120–1126 (1970).
Hirshfield, I. N., Rosenfeld, H. J., Leifer, Z., Maas, W. K.: Isolation and characterization of a mutant of Escherichia coli blocked in the synthesis of putrescine. J. Bact. 101, 725–730 (1970).
Hong, J., Ames, B. N.: Localized mutagenesis of any specific small region of the bacterial chromosome. Proc. nat. Acad. Sci. (Wash.) 68, 3158–3162 (1971).
Kadner, R. J., Maas, W. K.: Regulatory gene mutations affecting arginine biosynthesis in Escherichia coli. Molec. gen. Genet. 111, 1–14 (1971).
Lawrence, D. A., Smith, D. A., Rowbury, R. J.: Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to unhibition by analogues of methionine. Genetics 58, 473–492 (1968).
Maas, W. K.: Genetic defects affecting an arginine permease and repression of arginine synthesis in Escherichia coli. Fed. Proc. 23, 1239–1242 (1965).
Maas, W. K., Leifer, Z., Poindexter, J.: Studies with mutants blocked in the synthesis of polyamines. Ann. N.Y. Acad. Sci. 171, 957–967 (1970).
Tabor, H., Tabor, C. W.: Partial separation of two pools of arginine in Escherichia coli; preferential use of exogenous rather than endogenous arginine for the biosynthesis of 1,4-diaminobutane. J. biol. Chem. 244, 6383–6387 (1969).
Taylor, A. L.: Current linkage map of Escherichia coli. Bact. Rev. 34, 155–175 (1970).
Author information
Authors and Affiliations
Additional information
Communicated by E. Witkin
Rights and permissions
About this article
Cite this article
Maas, W.K. Mapping of genes involved in the synthesis of spermidine in Escherichia coli . Molec. Gen. Genet. 119, 1–9 (1972). https://doi.org/10.1007/BF00270439
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00270439