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A new method for the purification of the different stages of carrot embryoids

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Abstract

An easy method is presented for the purification of the different stages of carrot embryoids. This is based on a synchronization of the regenerating culture and on a filtration through filters of various pore sizes. A differential sedimentation was used for removing undifferentiated cells. At the end of the process, the different stages: globular, heart- and torpedo-shaped were obtained with a degree of purity that always exceeded 90%. This method can be used for the separation of relatively large numbers of embryoids (from thousands to a million) of haploid and diploid carrot lines and is very gentle on embryoids in that it does not affect their viability or further development.

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Abbreviations

6BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

pol:

polymerase

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Authors and Affiliations

  1. Istituto di Mutagenesi e Differenziamento, C.N.R., Via Svezia, 10, I-56100, Pisa, Italy

    G. Giuliano, D. Rosellini & M. Terzi

Authors
  1. G. Giuliano
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  2. D. Rosellini
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  3. M. Terzi
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Communicated by D. von Wettstein

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Giuliano, G., Rosellini, D. & Terzi, M. A new method for the purification of the different stages of carrot embryoids. Plant Cell Reports 2, 216–218 (1983). https://doi.org/10.1007/BF00270108

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  • DOI: https://doi.org/10.1007/BF00270108

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