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Isolation of the Cell Wall

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Isolation of Plant Organelles and Structures

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1511))

Abstract

This chapter describes a method allowing the purification of the cell wall for studying both polysaccharides and proteins. The plant primary cell wall is mainly composed of polysaccharides (90–95 % in mass) and of proteins (5–10 %). At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %). Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification. It plays critical roles during plant development and in response to environmental constraints. It is largely used in the food and textile industries as well as for the production of bioenergy. All these characteristics and uses explain why its study as a true cell compartment is of high interest. The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material. Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided.

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Acknowledgments

The authors are grateful to the Université Paul Sabatier (Toulouse III, France) and CNRS for support. They also wish to thank Carole Pichereaux for providing access to MALDI-TOF MS analysis at the Proteomics platform of GenoToul (http://proteomique.genotoul.fr/). Kahina Merah, Huan Nguyen-Kim, and Vincent Hervé are acknowledged for communication of unpublished data.

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Authors and Affiliations

  1. Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, 24 chemin de Borde Rouge, Auzeville, BP 42617, 31326 Castanet, Tolosan, France

    Hervé Canut, Cécile Albenne & Elisabeth Jamet

Authors
  1. Hervé Canut
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  2. Cécile Albenne
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  3. Elisabeth Jamet
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Corresponding author

Correspondence to Elisabeth Jamet .

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Editors and Affiliations

  1. The University of Western Australia, Perth, West Australia, Australia

    Nicolas L. Taylor

  2. The University of Western Australia, Perth, West Australia, Australia

    A. Harvey Millar

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Canut, H., Albenne, C., Jamet, E. (2017). Isolation of the Cell Wall. In: Taylor, N., Millar, A. (eds) Isolation of Plant Organelles and Structures. Methods in Molecular Biology, vol 1511. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6533-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6533-5_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6531-1

  • Online ISBN: 978-1-4939-6533-5

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