Summary
When 30S ribosomal subunits from E. coli are incubated with poly U, two separable components are recovered by zonal centrifugation of the incubation mixture. The faster sedimenting component is an aggregate of 30S subunits and poly U, while the slower one corresponds to the 30S ribosomal subunit. One ribosomal protein, protein 30S-1 is predominantly present in the faster sedimenting aggregate. The amount of poly U-30S subunit complex formed in the incubation mixture is limited by the amount of protein 30S-1 present. Consequently the number of ribosomal binding sites available for Phe-tRNA is limited in a similar fashion by the presence of protein 30S-1. When 30S ribosomal subunits are reconstituted in the absence of protein 30S-1, very little poly U or Phe-tRNA binding capacity is manifest under our assay conditions. We conclude that protein 30S-1 is required for maximum capacity of ribosomes to bind mRNA. Since this protein is present only on a fraction of the ribosome at any one time, it must exchange from one ribosome to another during protein synthesis.
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Abbreviations
- Poly U:
-
(polyuridylic acid)
- t-RNA:
-
(transfer ribonucleic acid)
- mRNA:
-
(messenger ribonucleic acid)
- Phe:
-
(phenylanine)
- A260 unit:
-
(unit of material which gives an optical density of 1.0 at 260 nm in a one centimeter optical path)
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Communicated by H. G. Wittmann
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Van Duin, J., Kurland, C.G. Functional heterogeneity of the 30S ribosomal subunit of E. coli . Molec. Gen. Genet. 109, 169–176 (1970). https://doi.org/10.1007/BF00269653
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DOI: https://doi.org/10.1007/BF00269653