Abstract
Cultural systems developed for the successful production of haploid plants from anther and microspore culture of a number of species are lethal to cotton microspores. Components of these systems were examined individually and in simple combinations to determine their specific effect on cotton microspore viability during isolation and culture. An organically buffered pH of 7.0 was critical for survival. The addition of potassium salts, near standard concentrations used for other species, enhanced survival and the cytoplasmic appearance of isolated microspores. Severe toxicity resulted from ammonium, calcium and magnesium salts, and these ions were tolerated only at very low concentrations. Iron, glutamine, serine, inositol, vitamins, and trace minerals were generally not detrimental to microspores at standard concentrations. An isolation and cultural maintenance system was developed that yields large quantities of healthy, viable cotton microspores. This initial step allows for further research in inducing cotton microspores to divide and undergo embryogenesis.
Similar content being viewed by others
Abbreviations
- ACES:
-
N-2-Acetamido-2-aminoethanesulfonic acid
- ADA:
-
N-2-Acetamidoiminodiacetic acid
- BES:
-
N,N-bis-(2-hydroxethyl)-2-aminoethanesulfonic acid
- HEPES:
-
N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid
- MOPS:
-
3-(N- Morpholino) propanesulfonic acid
- TES:
-
2- tris-(hydroxymethyl) methyl-aminoethanesulfonic acid
- PIPES:
-
Piperazine-N,N′-bis(2-ethanesulfonic acid) dipotassium salt
References
Barrow JR, Chaudhari HK (1976) Crop Sci. 16:441–442
Barrow JR, Katterman F, Williams D (1978) Crop Sci. 18:619–622
Bourgin JP, Nitsch JP (1967) Ann. Physiol. Veg. 9:377–382
Davidonis GH, Hamilton RH (1983) Plant Sci. Lett. 32:89–93
Davis DG, Dusebek KE, Hoerauf RA (1974) In Vitro 9:395–398
Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50:151–158
Linsmaier EM, Skoog F (1965) Physiol. Plant. 18:100–127
Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497
Nitsch D (1974) In: Kasha Kf (ed) Haploids in Higher Plants-Advances and Potential, Univ of Guelph, pp 123–135
Oswald TH, Nicholson RL, Bauman LF (1977) Physiol. Plant. 41:45–50
Price HJ, Smith (1979) Planta 145:305–307
Price HJ, Smith RH, Grumbles RM (1979) Plant Sci. Lett. 10:115–119
Rani A, Bhojwani SS (1976) Plant Sci. Lett. 7:163–169
Reinert J, Bajaj YPS (1977) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer-Verlag, New York
Smith RH, Price HJ, Thaxton JB (1977) In Vitro 13:329–334
Turcotte EL, Feaster CV (1967) J. Hered. 58:55–57
Vasil IK (1980) In: Vasil IK (ed) International review of cytology, Suppl 11A, Perspectives in plant cell and tissue culture, Academic Press, New York, pp 195–293
Author information
Authors and Affiliations
Additional information
Communicated by G. C. Phillips
USDA-ARS, in cooperation with the New Mexico Agricultural Experimental Station, Las Cruces, NM 88003, USA. This paper is published as Journal Article No. 1012
Rights and permissions
About this article
Cite this article
Barrow, J.R. The conditions required to isolate and maintain viable cotton (Gossypium hirsutum L.) microspores. Plant Cell Reports 5, 405–408 (1986). https://doi.org/10.1007/BF00269627
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269627