Summary
The lethal and cytoplasmic mutagenic effects of 52°C incubation during the cell cycle of a haploid strain of Saccharomyces cerevisiae were examined. Both effects varied periodically in a rather parallel pattern: the maximum thermosensitivity was seen at budding time, corresponding to the S period (Williamson, 1965). The 52°C induction of a nuclear forward mutation was also examined: canavanine-resistant mutants were induced by this treatment. Exponentially growing cells were much more sensitive than resting cells to the different effects of heating which were studied. On the other hand, on comparing asynchronous cultures of 6 different radiosensitive mutants only one (xrs5) showed a greater thermosensitivity than the corresponding wild type.
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Communicated by B. A. Bridges
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Schenberg-Frascino, A., Moustacchi, E. Lethal and mutagenic effects of elevated temperature on haploid yeast. Molec. Gen. Genet. 115, 243–257 (1972). https://doi.org/10.1007/BF00268888
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DOI: https://doi.org/10.1007/BF00268888