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Genetic and metabolic regulation of purine base transport in Neurospora crassa

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Summary

Neurospora crassa can utilize various purine bases such as xanthine or uric acid and their catabolic products as a nitrogen source. The early purine catabolic enzymes in this organism are regulated by induction and by ammonium repression. Studies were undertaken to investigate purine base transport and its regulation in Neurospora. The results of competition experiments with uric acid and xanthine transport strongly suggest that uric acid and xanthine share a common transport system. It was also shown that the common transport system for uric acid and xanthine is distinct from a second transport system shared by hypoxanthine, adenine and guanine, and apparently also distinct from the transport system(s) for adenosine, cytosine and uracil. Regulation of the uric acid-xanthine transport system and the hypoxanthine-adenine-guanine transport system was studied. The results reveal that the uric acid-xanthine transport system is regulated by ammonium repression, but does not require uric acid induction. Neither ammonium repression nor uric acid induction controls the hypoxanthine-adenine-guanine transport system. A gene, designated amr, which is believed to be a positive regulatory gene for nitrogen metabolism of Neurospora crassa, was found to dramatically affect both the uric acid-xanthine transport system and the hypoxanthine-adenine-guanine transport system. A model for the action of the amr locus as a positive regulatory gene and for the interaction between the amr gene product and its recognition sites will be discussed.

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References

  • Arst, H.N., Cove, D.J.: Nitrogen metabolic repression in Aspergillus nidulans. Molec. gen. Genet. 126, 111–141 (1973)

    Google Scholar 

  • Cummins, J.E., Mitchison, J.M.: Adenine uptake and pool formation in the fission yeast Schizosaccharomyces pombe. Biochim. biophys. Acta (Amst.) 136, 108–120 (1967)

    Google Scholar 

  • Darlington, A.J., Scazzocchio, C.: Use of analogues and the substrate-sensitivity of mutants in the analysis of purine uptake and breakdown in Aspergillus nidulans. J. Bact. 93, 937–940 (1967)

    Google Scholar 

  • Magill, J.M., Magill, C.W.: Purine base transport in Neurospora crassa. J. Bact. 124, 149–154 (1975)

    Google Scholar 

  • Magill, J.M., Spencer, R.R., Magill, C.W.: Relationship between (8-14C) adenosine transport and growth inhibition in Neurospora crassa strain ad-8. J. Bact. 119, 202–206 (1974)

    Google Scholar 

  • Pickering, W.R., Woods, R.A.: The uptake and incorporation of purines by wild-type Saccharomyces cerevisiae and a mutant resistant to 4-amino-pyrazolo (3, 4-d) pytidmidine. Biochim. biophys. Acta (Amst.) 264, 45–58 (1972)

    Google Scholar 

  • Polak, A., Grenson, M.: Evidence for a common transport system for cytosine, adenine and hypoxanthine in Saccharomyces cerevisiae and Candida albicans. Europ. J. Biochem. 32, 276–282 (1973)

    Google Scholar 

  • Pourquie, J.: Antagonism by adenine in the nutrition of Schizosaccharomyces pombe mutants inhibition at the level of guanine uptake. Biochim. biophys. Acta (Amst.) 209, 269–277 (1970)

    Google Scholar 

  • Reichert, U., Winter, M.: Uptake and accumulation of purine bases by stationary yeast cells pretreated with glucose. Biochim. biophys. Acta (Amst.) 356, 108–116 (1974)

    Google Scholar 

  • Reinert, W.R., Marzluf, G.A.: Regulation of the purine catabolic enzymes in Neurospora crassa. Arch. Biochem. Biophys. 166, 565–574 (1975a)

    Google Scholar 

  • Reinert, W.R., Marzluf, G.A.: Genetic and metabolic control of the purine catabolic enzymes of Neurospora crassa. Molec. gen. Genet. 139, 39–55 (1975b)

    Google Scholar 

  • Schlitz, J.R., Terry, K.D.: Nucleoside uptake during the germination of Neurospora crassa conidia. Biochim. biophys. Acta (Amst.) 209, 278–288 (1970)

    Google Scholar 

  • Shavlovsky, G.M., Sibirny, A.A.: Regulation of uric acid uptake in the yeast Pichia guilliermondii. Fed. Europ. Biochem. Soc. Lett. 31, 313–316 (1973)

    Google Scholar 

  • Vogel, J.: A convenient growth medium for Neurospora (Medium N). Microb. Genet. Bull. 13, 42–43 (1956)

    Google Scholar 

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Authors and Affiliations

  1. Developmental Biology Program, The Ohio State University, Columbus, Ohio, U.S.A.

    Te-Fang Tsao & George A. Marzluf

  2. Department of Biochemistry, The Ohio State University, Columbus, Ohio, U.S.A.

    Te-Fang Tsao & George A. Marzluf

Authors
  1. Te-Fang Tsao
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  2. George A. Marzluf
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Communicated by F. Kaudewitz

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Tsao, TF., Marzluf, G.A. Genetic and metabolic regulation of purine base transport in Neurospora crassa . Molec. Gen. Genet. 149, 347–355 (1976). https://doi.org/10.1007/BF00268537

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  • DOI: https://doi.org/10.1007/BF00268537

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