Summary
We have constructed a strain of Salmonella typhimurium which contains the malB region from Escherichia coli and carries the bacteriophage λ receptor protein in its outer membrane. Phage λ adsorbs to this strain but cannot grow, thus providing a very useful system for transposon mutagenesis of S. typhimurium using λ vehicles carrying transposons. This system can also be used for cosmid cloning.
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Communicated by K. Isono
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Tapio Palva, E., Liljeström, P. & Harayama, S. Cosmid cloning and transposon mutagenesis in Salmonella typhimurium using phage λ vehicles. Molec. Gen. Genet. 181, 153–157 (1981). https://doi.org/10.1007/BF00268420
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DOI: https://doi.org/10.1007/BF00268420