Summary
The ability of serveral strains of Streptomyces to degrade cell walls from Fusarium scirpi was tested by plating them on agar containing a cell wall preparation derived from the fungus. In this assay, S. tsusimaensis was most effective in producing a clear zone of lysis during growth on the opaque medium. This Streptomyce strain was subsequently grown in liquid culture containing cell walls as the sole carbon source and the exoenzymes were isolated from the culture broth. The enzyme preparation produces a clear zone of lysis when filled into wells in the cell wall agar and was used to prepare protoplasts from F. scirpi. The protoplast yield was 1x109 protoplasts/ml of enzyme solution from 35 mg dry weight of Fusarium mycelium. Protoplasts could be regenerated at a frequency of up to 80%.
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Billich, A., Keller, U., Kleinkauf, H. et al. Production of protoplasts from Fusarium scirpi by lytic enzymes from Streptomyces tsusimaensis . Appl Microbiol Biotechnol 28, 442–444 (1988). https://doi.org/10.1007/BF00268211
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DOI: https://doi.org/10.1007/BF00268211