Summary
Among the survivors of Escherichia coli derivatives infected with phage λc1 or λvir that are unable to establish ordinal lysogeny, clones arise which perpetuate the nondefective phage genome. When the bacteria bears a mutation(s) that makes the cell tolerant to the phage multiplication, such clones appear readily.
The bacteria-λ complex was studied genetically and chemically, and it was concluded that the intact phage genomes, about two to four circular copies per bacterial chromosome, are perpetuated in bacterial cytoplasm as plasmids or in “lysogenic state in cytoplasm”.
Several lines of evidence suggests that the phage genome in the lysogenic state in cytoplasm is under a different regulatory system from that in the normal prophage state on chromosome.
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Communicated by H. Ozeki
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Takahashi, S., Matsubara, K. Plasmid formation from bacteriophage λ in Escherichia coli . Molec. Gen. Genet. 114, 281–289 (1972). https://doi.org/10.1007/BF00267496
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DOI: https://doi.org/10.1007/BF00267496