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Genetic studies of tolerance to colicin E2 in Escherichia coli K-12

II. Multiple mutations as a cause of the various phenotypic properties of Cet- mutants

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Summary

The genetic nature of some of the phenotypic properties of Cet- mutants, which were selected as being tolerant to colicin E2, has been examined. In contrast to other reports, tolerance to colicin E2, which derives from a mutation at the cet locus, was found to segregate from the other phenotypic properties of Cet- mutants in genetic crosses. Thus, in two Cet- strains, sensitivity to ultraviolet (UV) light derived from a lon mutation; in another, UV-sensitivity mapped near the recA locus; in a fourth mutant, resistance to phage T4 was shown to map at a position distinct from cet and loosely linked to his and tyrA. An investigation of one of the properties of certain Cet- strains, viz., resistance to phage λ, revealed it to be due to poor adsorption of the phage.

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Authors and Affiliations

  1. Department of Genetics, University of Leicester, Leicester, England

    R. S. Buxton & I. B. Holland

  2. Division of Microbiology, National Institute for Medical Research, Mill Hill, NW7 1AA, London, England

    R. S. Buxton

Authors
  1. R. S. Buxton
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  2. I. B. Holland
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Communicated by G. Bertani

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Buxton, R.S., Holland, I.B. Genetic studies of tolerance to colicin E2 in Escherichia coli K-12. Molec. Gen. Genet. 131, 159–171 (1974). https://doi.org/10.1007/BF00266151

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  • DOI: https://doi.org/10.1007/BF00266151

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