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Die Wirkung von Vasopressin auf die Konzentrationsgradienten von Natrium und Harnstoff im Nierengewebe bei verschiedenen Diuresezuständen

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Summary

The sodium and urea content of papillary and -cortical tissue in rat kidneys was determined at various diuretic stages, induced by thirst, oral respectively i.v. load with water, different concentrations of NaCl or Mannitol; in similar treated animals urine was collected and flow rate, osmolality, Na+, K+ and urea were determined as well as plasma osmolality at the end of the experiment. The effect of Vasopressin (VP, 100 mE/kg i.p.) was studied under these conditions.

After oral load with water VP injection increased Na+ and urea concentrations in papillary tissue; however after oral pretreatment with 0.9% NaCl, VP did not change papillary Na+ concentration and diminished papillary urea in spite of a shift in urinary osmolality from about isotonic to hypertonic values. Throughout the study changes in cortical tissue concentrations were small compared with those of the papilla.

When VP was injected to animals, pretreated with hypertonic NaCl solutions, no significant changes in papillary sodium and urea concentrations and no further concentration of urine osmolality occured, indicating maximal operation of the concentrating mechanism induced by pretreatment. In spite of this VP exerted natriuresis and enhanced urinary flow rate.

Following pretreatment with the different fluids, VP injection never resulted in papillary Na+, and urea-concentrations as high as in thirsting animals; likewise antidiuresis and urine osmolality were considerably smaller than in thirsting animals when VP was applicated to fluid-loaded animals.

The results are discussed with respect to factors with regulate the osmotical concentration in the kidney medulla as countercurrent multiplikation within the loop of Henle, medullary blood-flow and “washout” of medullary solutes following reabsorption of water out of the collecting ducts.

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  1. Aus der Pharmakologischen Abteilung der Arzneimittelforschung G.m.b.H., Wien XII, Österreich

    W. Kobinger & A. Walland

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  1. W. Kobinger
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  2. A. Walland
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Kobinger, W., Walland, A. Die Wirkung von Vasopressin auf die Konzentrationsgradienten von Natrium und Harnstoff im Nierengewebe bei verschiedenen Diuresezuständen. Naunyn - Schmiedebergs Arch 246, 538–551 (1964). https://doi.org/10.1007/BF00246307

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