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Über das Vorkommen von Katecholaminen und von 3,4-Dihydroxyphenylalanin (Dopa) im Auge

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Summary

1. The occurrence and distribution of catecholamines in the ocular tissue of the calf has been investigated by a fluorimetric method.

2. In the lens, in the vitreous humour and in the retina (without the pigment-epithelium) no significant amounts of noradrenaline have been detected. In the chorioideal layer, isolated together with the retinal pigment-epithelium, noradrenaline was found in concentrations from 0.01 to 0.15 μg/g wet tissue. The noradrenaline concentration in the iris was in the range of 0.19–0.66 μg/g wet tissue.

3. The adrenaline concentration, as estimated in one sample of iris tissue, was as low as 0.05 μg/g.

4. Another fluorescent catechol compound was found in the iris as well as in the chorioideal layer (isolated together with the retinal pigment-epithelium).

5. This catechol compound was found to be 3.4-dihydroxyphenylalanine (dopa), as identified by twodimensional paper chromatography, separation on ion-exchange and Al2O3 columns, colour reactions, fluorescent behaviour and by enzymatic decarboxylation to dopamine.

6. The dopa concentration in these tissues was in the range of 1.06 to 4.34 μg/g wet tissue, or even higher, as might be concluded from the experimental data.

7. The occurrence of dopa in the ocular tissue might possibly be connected with the metabolism of melanin.

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Authors and Affiliations

  1. Aus dem Pharmakologischen Institut der Universität Wien, Austria

    H. Bernheimer

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  1. H. Bernheimer
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Ein Teil der Ergebnisse wurde auf der 4. Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft in Mainz vorgetragen [Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 246, 12 (1963)].

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Bernheimer, H. Über das Vorkommen von Katecholaminen und von 3,4-Dihydroxyphenylalanin (Dopa) im Auge. Naunyn - Schmiedebergs Arch 247, 202–213 (1964). https://doi.org/10.1007/BF00245964

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  • DOI: https://doi.org/10.1007/BF00245964

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