Summary
A study of the p- and o-hydroxylation of aniline by rabbit-liver microsomes has shown that these reactions are differently affected by the storage of the microsomes and by copper chloride or semicarbazide. The results point to the existence of two enzymes involved in the p- and o-hydroxylation of aniline.
Similar content being viewed by others
References
Bray, H. G., R. C. Clowes, and W. V. Thorpe: The metabolism of aminophenols, o-formamidophenol, benzoxazole, 2-methyl- and 2-phenyl-benzoxazoles and benzoxazolone in the rabbit. Biochem. J. 51, 70 (1952).
Brodie, B. B., and J. Axelrod: The estimation of acetanilide and its metabolic products, aniline, N-acetyl p-aminophenol and p-aminophenol (free and total conjugated) in biological fluids and tissues. J. Pharmacol. exp. Ther. 94, 22 (1948).
Kampffmeyer, H., u. M. Kiese: Einige Eigenschaften der Anilin hydroxylierenden Enzyme in Mikrosomen. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 244, 375 (1963).
— Further factors affecting the hydroxylation of aniline and some of its derivatives by liver microsomes. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 246, 397 (1964a).
The hydroxylation of aniline and N-ethylaniline by microsomal enzymes at low oxygen pressures. Biochem. Z. (in press) (1964b).
Miller, E. C., and J. A. Miller: A mechanism of ortho-hydroxylation of aromatic amines in vivo. Biochim. biophys. Acta (Amst.) 40, 380 (1960).
Mitoma, Ch., H. S. Posner, H. C. Reiz, and S. Udenfriend: Enzymatic hydroxylation of aromatic compounds. Arch. Biochem. 61, 431 (1956).
Parke, D. V.: Studies in Detoxication. 84. The metabolism of [14C] aniline in the rabbit and other animals. Biochem. J. 77, 493 (1960).
muel-R. T. Williams: Species differences in the o- and p-hydroxylation of aniline. Biochem. J. 63, 12P (1956).
Piotrowski, J.: The metabolism of aniline in rabbits. Med. Pracy 12, 309 (1961). Chem. Abstr. 57, 2732 (1961).
Posner, H. S., Ch. Mitoma, and S. Udenfriend: Enzymic hydroxylation of aromatic compounds. II. Further studies of the properties of the microsomal hydroxylating system. Arch. Biochem. 94, 269 (1961).
Weisburger, J. H., E. K. Weisburger, and H. P. Morris: Orientation of biochemical hydroxylation in aromatic compounds. Science 125, 503 (1957).
— and H. A. Sober: Chromatographic separation of some metabolites of the carcinogen N-2-fluorenylacetamide. J. nat. Cancer Inst. 17, 363 (1956).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bauer, S., Kiese, M. Heterogeneousness of the microsomal enzymes effecting the o- and p-hydroxylation of aniline. Naunyn - Schmiedebergs Arch 247, 144–148 (1964). https://doi.org/10.1007/BF00245417
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00245417