The entrainment of rhythmically discharging reticulospinal neurons of the eel by sensory nerve stimulation

Abstract

Recordings made from decerebrated, paralyzed eels (Anguilla anguilla) producing rhythmical spinal motoneuronal activity showed that around 65% of identified reticulospinal units, belonging to the inferior reticular division, discharged rhythmically. The reticulospinal bursts, lasting from 300 up to 3000 ms, were in time with spinal motoneuronal bursting activity. In different fish the modal cycle period varied between 2 to 4 s and burst duration and firing frequency of each neuron showed large changes from cycle to cycle. Burst responses similar in form to those occurring spontaneously were evoked from reticular neurons when the ophthalmic nerve was stimulated regularly (intervals of 1 to 10 s) but the cycle period, firing frequency and burst duration were now more predictable. For stimulation intervals between 2 and 5 s, each ophthalmic nerve stimulus was normally followed by a burst from the reticulospinal neuron. The cycle period of the reticular rhythm then became equal to the interstimulus interval and the reticulospinal unit was entrained by the stimulus. Beyond this range of interstimulus intervals, complete entrainment was lost. We suggest that regular sensory input provides a powerful stabilising influence to rhythmically active motor systems in the brainstem and spinal cord.