Skip to main content
SpringerLink
Log in

In vitro morphogenic characteristics of phytochrome mutants in Nicotiana plumbaginifolia are modified and correlated to high indole-3-acetic acid levels

  • Published:
Planta Aims and scope Submit manuscript

Abstract

The involvement of indole-3-acetic acid (IAA) in the integration of the light signal perceived by phytochrome during the morphogenesis of plants was investigated in Nicotiana plumbaginifolia Viviani. The chromophore mutant pew1, deficient in all the phytochrome types, and the aurea-like mutant pew2, which appears to be specifically deficient in phytochromes expressed in darkness, were analysed for IAA-related morphogenic effects such as rooting, shooting and callus formation. We observed, in the absence of exogenously applied hormones, abundant root formation by the pew2 mutant. The pew1 mutant exhibited callus formation in the presence of gibberellins and cytokinins when the wild type did not. The previously described lethality of the double mutant pew1–pew2 was shown to be hormone-dependent since, in the light, exogenously applied auxin and cytokinin (0.1 mg·1−1 each) led to plant regeneration from calli and subsequent normal development. These observations suggested an increase in the auxin/cytokinin ratio as a consequence of the phytochrome mutations. We correlated these morphogenic characteristics with high IAA levels in the mutants. The difference in IAA accumulation in the two mutants indicates that among the different phytochromes expressed by N. plumbaginifolia, the light-expressed isoforms play a major role in the control of IAA levels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

N6-benzyladenine

CK:

cytokinin

GA3 :

gibberellic acid

iP:

isopentenyladenine

NAA:

α-naphthalene acetic acid

pew :

partly etiolated in white light

Z:

zeatin

References

  • Amritphale, D., Singh, B., Gutch, A. (1992) Inhibition of red lightinduced seed germination by indole-3-acetic acid in Hygrophila auriculata. J. Plant Growth Regul. 11, 203–208

    Google Scholar 

  • Behringer, F.J., Davies, P.J. (1992) Indole-3-acetic acid levels after phytochrome-mediated changes in the stem elongation rate of dark-grown and light-grown Pisum seedlings. Planta 188, 85–92

    Google Scholar 

  • Besse, I., Verdeil, J.L., Duval, Y., Sotta, B., Maldiney, R., Miginiac, E. (1992) Oil Palm (Elaeis guineensis Jacq.) clonal fidelity: endogenous cytokinins and indoleacetic acid in embryogenic callus cultures. J. Exp. Bot. 43, 983–989

    Google Scholar 

  • Bourgin, J.-P., Chupeau, Y., Missonier, C. (1979) Plant regeneration from mesophyll protoplasts of several Nicotiana species. Physiol. Plant. 45, 288–292

    Google Scholar 

  • Bowler, C., Neuhaus, G., Yamagata, H., Chua, N.H. (1994) Cyclic GMP and calcium mediate phytochrome phototransduction. Cell 77, 73–81

    Google Scholar 

  • Casal, J.J., Sanchez, R.A., Vierstra, R.D. (1994) Avena phytochrome-A overexpressed in transgenic tobacco seedlings differentially affects red/far-red reversible and very-low-fluence responses (cotyledon unfolding) during de-etiolation. Planta 192, 306–309

    Google Scholar 

  • Chory, J. (1993) Out of darkness: mutants reveal pathways controlling light-regulated development in plants. TIG 9, 167–172

    Google Scholar 

  • Chory, J., Reinecke, D., Sim, S., Washburn, T., Brenner, M. (1994) A role for cytokinins in de-etiolation in Arabidopsis. Det mutants have an altered response to cytokinins. Plant. Physiol. 104, 339–347

    Google Scholar 

  • Deng, X.W. (1994) Fresh view of light signal transduction in plants. Cell 76, 423–426

    Google Scholar 

  • Halliday, K.J., Koornneef, M., Whitelam, G.C. (1994) Phytochrome B and at least one other phytochrome mediate the accelerated flowering response of Arabidopsis thaliana L. to low red/far-red ratio. Plant. Physiol. 104, 1311–1315

    Google Scholar 

  • Iino, M. (1982a) Inhibitory action of red light on the growth of the maize mesocotyl: evaluation of the auxin hypothesis. Planta 156, 388–395

    Google Scholar 

  • Iino, M. (1982b) Action of red light on indole-3-acetic-acid status and growth in coleoptiles of etiolated maize seedlings. Planta 156, 21–32

    Google Scholar 

  • Jones, A.M., Cochran, D.S., Lamerson, P.M., Evans, M.L., Cohen, J.D. (1991) Red light-regulated growth. I. Changes in the abundance of indoleacetic acid and a 22-kilodalton auxin-binding protein in the maize mesocotyl. Plant. Physiol. 97, 352–358

    Google Scholar 

  • Julliard, J., Sotta, B., Pelletier, G., Miginiac, E. (1992) Enhancement of naphthaleneacetic acid-induced rhizogenesis in Tl-DNA-trans-formed Brassica napus without significant modification of auxin levels and auxin sensitivity. Plant. Physiol. 100, 1277–1282

    Google Scholar 

  • Kendrick, E.K., Nagatani, A. (1991) Phytochrome mutants. Plant J. 1, 133–139

    Google Scholar 

  • Kopcewicz, J., Cymerski, M., Madela, K. (1987) Phytochrome and IAA in the control of oat coleoptile elongation. Acta Physiol. Plant. 9, 69–76

    Google Scholar 

  • Kraepiel, Y., Jullien, M., Cordonnier-Pratt, M.-M., Pratt, L. (1994) Identification of two loci involved in phytochrome expression in Nicotiana plumbaginifolia and lethality of the corresponding double mutant. Mol. Gen. Genet. 242, 559–565

    Google Scholar 

  • Maldiney, R., Leroux, B., Sabbagh, I., Sotta, B., Sossountzov, L., Miginiac, E. (1986) A biotin-avidin-based enzyme immunoassay to quantify three phytohormones: auxin, abscisic acid and zeatin-riboside. J. Immunol. Methods 90, 151–158

    Google Scholar 

  • McCormac, A.C., Wagner, D., Boylan, M.T., Quail, P.H., Smith, H., Whitelam, G.C. (1993) Photoresponses of transgenic Arabidopsis seedlings expressing introduced phytochrome B-encoding cDNAs: evidence that phytochrome A and phytochrome B have distinct photoregulatory functions. Plant J 4, 19–27

    Google Scholar 

  • Nagatani, A., Reid, J.B., Ross, J.J., Dunnewijk, A., Furuya, M. (1990) Internode length in Pisum: the response to light quality, and phytochrome type 1 and 2 levels in lv plants. J. Plant Physiol. 135, 667–674

    Google Scholar 

  • Nagatani, A., Chory, J., Furuya, M. (1991) Phytochrome-B is not detectable in the hy3 mutant of Arabidopsis, which is deficient in responding to end-of-day far-red light treatments. Plant Cell Physiol. 32, 1119–1122

    Google Scholar 

  • Nagatani, A., Reed, J.W., Chory, J. (1993) Isolation and initial characterization of Arabidopsis mutants that are deficient in phytochrome-A. Plant Physiol. 102, 269–277

    Google Scholar 

  • Neff, M.M., Van Volkenburgh, E. (1994) Light-stimulated cotyledon expansion in Arabidopsis seedlings. The role of Phytochrome B. Plant Physiol. 104, 1027–1032

    Google Scholar 

  • Neuhaus, G., Bowler, C., Kern, R., Chua, N.H. (1993) Calcium/calmodulin-dependent and-independent phytochrome signal transduction pathways. Cell 73, 937–952

    Google Scholar 

  • Parks, B.M., Quail, P.H. (1991) Phytochrome-deficient hy1 and hy2 long hypocotyl mutants of Arabidopsis are defective in phytochrome chromophore biosynthesis. Plant Cell 3, 1177–1186

    Google Scholar 

  • Parks, B.M., Quail, P.H. (1993) Hy8, a new class of Arabidopsis long hypocotyl mutants deficient in functional phytochrome-A. Plant Cell 5, 39–48

    Google Scholar 

  • Reed, J.W., Nagatani, A., Elich, T.D., Fagan, M., Chory, J. (1994) Phytochrome A and phytochrome B have overlapping but distinct functions in Arabidopsis development. Plant Physiol. 104, 1139–1149

    Google Scholar 

  • Reinecke, D.M., Bandurski, A.S. (1987) Auxin biosynthesis and metabolism. In: Plant hormones and their role in plant growth and development, Davies, P.J., ed. Martinus Nijhoff Publishers, Dordrecht pp. 24–42

    Google Scholar 

  • Sharma, R., Lopez-Juez, E., Nagatani, A., Furuya, M. (1993) Identification of photo-inactive phytochrome A in etiolated seedlings and photo-active phytochrome B in green leaves of the aurea mutant of tomato. Plant J. 4, 1035–1042

    Google Scholar 

  • Sharrock, R.A., Quail, P.H. (1989) Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Dev. 3, 1745–1757

    Google Scholar 

  • Shinomura, T., Nagatani, A., Chory, J., Furuya, M. (1994) The induction of seed germination in Arabidopsis thaliana is regulated principally by phytochrome B and secondarily by phytochrome A. Plant Physiol. 104, 363–371

    Google Scholar 

  • Sotta, B., Pilate, G., Pelèse, F., Sabbagh, I., Bonnet, M., Maldiney, R. (1987) An avidin-biotin solid phase ELISA for femtomole isopentenyladenine and isopentenyladenosine measurements in HPLC purified plant extracts. Plant Physiol. 84, 571–573

    Google Scholar 

  • Walton, J.D., Ray, P.M. (1981) Evidence for receptor function of auxin binding sites in maize. Red light inhibition of mesocotyl elongation and auxin binding. Plant. Physiol. 68, 1334–1338

    Google Scholar 

  • Whitelam, G.C., Johnson, E., Peng, J.R., Carol, P., Anderson, M.L., Cowl, J.S., Harberd, N.P. (1993) Phytochrome-A null mutants of Arabidopsis display a wild-type phenotype in white light. Plant Cell 5, 757–768

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Physiologie du Développement des Plantes URA 1180 CNRS, Université Pierre et Marie Curie, case 156, F-75252, Paris Cedex 05, France

    Yvan Kraepiel, Karine Marree, Bruno Sotta & Emile Miginiac

  2. Laboratoire de Biologie Cellulaire, INRA Versailles, F-78026, Versailles Cedex, France

    Yvan Kraepiel & Michel Caboche

Authors
  1. Yvan Kraepiel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karine Marree
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bruno Sotta
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michel Caboche
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Emile Miginiac
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Emile Miginiac.

Additional information

We thank Marc Jullien, Anne-Marie Lescure and Kirk Schnorr (Laboratoire de Biologie Cellulaire, INRA, Versailles, France) for critical reading of the manuscript, and Yvette Habricot (Laboratoire de Physiologie du Développement des Plantes, Université P. et M. Curie, Paris, France) and Bernard Renouf (Laboratoire de Biologie Cellulaire, INRA) for technical assistance.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kraepiel, Y., Marree, K., Sotta, B. et al. In vitro morphogenic characteristics of phytochrome mutants in Nicotiana plumbaginifolia are modified and correlated to high indole-3-acetic acid levels. Planta 197, 142–146 (1995). https://doi.org/10.1007/BF00239950

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00239950

Key words

Search

Navigation