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Gut regulatory peptides in some Antarctic notothenioids

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Abstract

The presence and the comparative distribution of regulatory peptides and serotonin in the gut of four species of Antarctic notothenioid fishes [Cryodraco antarcticus and Chionodraco hamatus (Channichthyidae), and Trematomus bernacchii and Trematomus newnesi (Nototheniidae)], were immunohistochemically studied. In these species, numerous immunoreactive (IR) endocrine cells and nerve elements were detected. In the nototheniids most of the IR nerve fibres were of the intrinsic type, while most of the IR nerve fibres of the channichthyid intestine, besides insulin-like IR fibres, seemed to be of the extrinsic type. The intensity and frequency of immunopositivity are not the same in channnichthyids and nototheniids; the species belonging to the first family show many differences from the teleosts living in temperate water. The finding of insulin-like endocrine cells and nerve fibres in the gut wall of Cryodraco antarcticus is exceptional for vertebrates and deserves special attention.

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References

  • Aldman G, Holmgren S (1987) Control of gallbladder motility in the rainbow trout, Salmo gairdneri. Fish Physiol Biochem 92C: 103–108

    Google Scholar 

  • Anderson C, Campbell G (1988) Immunohistochemical study of 5-HT-containing neurons in the teleost intestine: relationship to the presence of enterochromaffin cells. Cell Tissue Res 254: 553–559

    Google Scholar 

  • Beorlegui C, Martinez A, Sesma P (1992) Some peptide-like colocalizations in endocrine cells of the pyloric caeca and the intestine of Oncorhynchus mykiss (Teleostei). Cell Tissue Res 269:353–357

    Google Scholar 

  • Bishop AE, Polak JM, Yiangou Y, Christofides ND, Bloom SR (1984) The distribution of PHI and VIP in porcine gut and their co-localization to a proportion of intrinsic ganglion cells. Peptides 5:255–259

    Google Scholar 

  • Bjenning C, Holmgren S (1988) Neuropeptides in the fish gut. An immunohistochemical study of evolutionary patterns. Histochemistry 88:155–163

    Google Scholar 

  • Cimini V, Van Noorden S, Giordano-Lanza G, Nardini V, McGregor GP, Bloom SR, Polak JM (1985) Neuropeptides and 5-HT immunoreactivity in the gastric nerves of the dogfish (Scyliorhinus stellaris). Peptides 6[Suppl 3]:373–377

    Google Scholar 

  • Cimini V, Van Noorden S, Nardini V (1989) Peptides of the gastrointestinal tract of the dogfish (Scyliorhinus stellaris). J Exp Zool [Suppl 2]: 146–157

  • Conlon JM (1989) Biosynthesis of regulatory peptides: evolutionary aspects. In: Holmgren S (ed) The comparative physiology of regulatory peptides. Chapman & Hall, London, pp 344–369

    Google Scholar 

  • Conlon JM, Bjenning C, Moon TW, Youson JH, Thim L (1991) Neuropeptide Y-related peptides from the pancreas of a teleostean (eel), holostean (bowfin) and elasmobranch (skate) fish. Peptides 12:221–226

    Google Scholar 

  • Cutfield JF, Cutfield SM, Came A, Emdin SO, Falkmer S (1986) Isolation, purification and amino acid sequence of insulin from the teleost fish Cottus scorpius (Daddy sculpin). Eur J Biochem 158:117–123

    Google Scholar 

  • Devaskar SU, Singh BS, Carnaghi LR, Rajakumar PA, Giddings SJ (1993) Insulin II gene expression in rat central nervous system. Regul Peptides 48:55–63

    Google Scholar 

  • Eastman JT (1988) Lipid storage system and the biology of two neutrally buoyant antarctic notothenioid fishes. Comp Biochem Physiol 908:529–538

    Google Scholar 

  • Eastman JT (1991) Evolution and diversification of Antarctic notothenioid fishes. Am Zool 31:93–109

    Google Scholar 

  • Ekblad E, Hakanson R, Sundler F (1984) VIP and PHI coexist with an NPY-like peptide in intramural neurones of the small intestine. Regul Peptides 10:47–55

    Google Scholar 

  • Ekblad E, Alumets J, Elkman R, Falkmer S, Hakanson R, Sundler F (1985) Two distinct VIP precursors in cartilaginous fish? Peptides 6 [Suppl 3]:383–388

    Google Scholar 

  • Elbal MT, Lozano MT, Agulleiro B (1988) The endocrine cells in the gut of Mugil saliensis Risso 1810 (Teleostei); An immunocytochemical and ultrastructural study. Gen Comp Endocrinol 70:231–246

    Google Scholar 

  • El-Salhy M (1984) Immunocytochemical investigation of the gastroentero-pancreatic (GEP) neurohormonal peptides in the pancreas and gastrointestinal tract of the dogfish Squalus acanthias. Histochemistry 80:193–205

    Google Scholar 

  • Fujita T, Kanno T, Kobayashi S (1988) The paraneuron Springer Berlin Heidelberg New York, Tokyo, pp 165–184

    Google Scholar 

  • Gapp DA, Kenney MP (1985) The gastro-entero-pancreatic system of the turtle Crysemys picta. Peptides 6 [Suppl 1]:347–352

    Google Scholar 

  • Gapp DA, Polak JM (1990) Localization of insulin to gastroenteropancreatic cells in the turtle gastrointestinal tract. Gen Comp Endocrinol 78:48–55

    Google Scholar 

  • Gon O, Heemstra PC (eds) (1990) Fishes of the Southern Ocean. J LB Smith Institute of Ichthyology Grahamstown, South Africa, pp 279–399

    Google Scholar 

  • Holmgren S (1989) Gut motility. In: Holmgren S (ed) The comparative physiology of regulatory peptides Chapman & Hall, New York, pp 231–255

    Google Scholar 

  • Holmgren S, Nilsson S (1983) Bombesin-gastrin/CCK-5-hydroxytryptamine-neurotensin-somatostatin-and VIP-like immunoreactivity and catecholamine fluorescence in the gut of elasmobranch, Squalus acanthias. Cell Tissue Res 234:595–618

    Google Scholar 

  • Holmgren S, Jonsson A-C, Holstein B (1986) Gastrointestinal peptides in fish. In: Nilsson S, Holmgren S (eds) Fish physiology: Recent advances. Croom Helm, London, pp 119–139

    Google Scholar 

  • Jensen J, Holmgren S (1991) Tachykinins and intestinal motility in different fish groups. Gen Comp Endocrinol 83:388–396

    Google Scholar 

  • Jonsson AC, Holmgren S (1989) Gut secretion. In: Holmgren, S (ed) The comparative physiology of regulatory peptides. Chapman & Hall, New York, pp 256–271

    Google Scholar 

  • Jonsson AC, Holmgren S, Holstein B (1987) Gastrin/CCK-like immmoreactivity in endocrine cells and nerves in the gastrointestinal tract of the cod, Gadus morhua, and the effect of peptides of the gastrin/CCK family on cod gastrointestinal smooth muscle. Gen Comp Endocrinol 66:190–202

    Google Scholar 

  • Kiliaan AJ, Holmgren S, Jonsson A-C, Dekker K, Groot J (1992) Neurotensin, substance P, gastrin/cholecystokinin, and bombesin in the intestine of Tilapia (Oreochromis mossambicus) and the goldfish (Carassius auratus): Immunochemical detection and effects on electrophysiological characteristics. Gen Comp Endocrinol 88:351–363

    Google Scholar 

  • Kiliaan AJ, Holmgren S, Jonsson A-C, Dekker K, Groot JA (1993) Neuropeptides in the intestine of two teleost species (Oreochromis mossambicus, Carassius auratus): Localization and electrophysiological effects on the epithelium. Cell Tissue Res 271:123–134

    Google Scholar 

  • Langer M, Van Noorden S, Polak JM, Pearse AGE (1979) Peptide hormone-like immunoreactivity in the gastrointestinal tract and endocrine pancreas of eleven teleost species. Cell Tissue Res 199:493–508

    Google Scholar 

  • Larsson LI, Goltermann N, De Magistris L, Rehfeld JF, Schwartz TW (1979) Somatostatin cell processes as pathways for paracrine secretion. Science 205:1393–1395

    Google Scholar 

  • Macdonald JA, Montgomery JC, Wells RMG (1988) The physiology of McMurdo Sound fishes—current New Zealand research. Comp Biochem Physiol 90 B:567–578

    Google Scholar 

  • Masini MA, Uva-Ghiani B, Napoli L, Devecchi M (1992) Epithelia involved in ion-water transport in Antarctic fish. Abstracts Meeting “Animal and Related Cell” Soc. Exp. Biol., Lancaster, 5–10 April 1992 p. 44

  • Nicol JAC (1952) Autonomic nervous system in lower chordates. Bid Rev Cambridge Philos Soc 27:1–49

    Google Scholar 

  • Noaillac-Depeyre J, Hollande E (1981) Evidence for somatostatin, gastrin and pancreatic polypeptide-like substance in the mucosa cells of the gut in fishes with and without stomach. Cell Tissue Res 216:193–203

    Google Scholar 

  • Perez-Tomas R, Ballesta J, Pastor LM, Hernandez F (1989) Ultrastructural study of the endocrine cells of the gut of Testudo grueca (Chelonia). Anat Embryol 180:103–108

    Google Scholar 

  • Plisetskaya EM (1989) Pancreatic peptides. In: Holmgren S (ed) The comparative physiology of regulatory peptides. Chapman & Hall, London, pp 174–202

    Google Scholar 

  • Plisetskaya EM, Pollock HG, Rouse JB, Hamilton JW, Kimmel LR, Andrews PC, Gorbman A (1986) Characterization of coho salmon (Oncorhyncus kisutch) islet somatostatins. Gen Comp Endocrinol 63:252–263

    Google Scholar 

  • Pollock HG, Kimmel JR, Ebner KE, Hamilton JW, Rouse JB, Lance V, Rawitch AB (1988) Isolation of alligator gar (Lepisosteus spatula) glucagon, oxyntomodulin, and glucagon-like peptide: amino acid sequences of oxyntomodulin and glucagon-like peptide. Gen Comp Endocrinol 69:133–140

    Google Scholar 

  • Schwartz MW, Figlewicz DP, Baskin DG, Woods SC, Porte JD (1992) Insulin in the brain: A hormonal regulator of energy balance. Endocrinol Rev 13:387–414

    Google Scholar 

  • Sternberger LA (1979) Immunocytochemistry, 2nd edn. Wiley, New York, pp 104–169

    Google Scholar 

  • Tagliafierro G (1986) Localization, distribution, and ontogeny of vasoactive intestinal polypeptide-like and peptide histidine isoleucine immunoreactivities in the gastrointestinal tract of a cartilaginous fish. Can J Physiol Pharmacol 64 [Suppl]:26–27

    Google Scholar 

  • Tagliafierro G, Faraldi G, Bandelloni R (1985) Distribution, histochemistry and ultrastructure of somatostatin-like immunoreactive cells in the gastroenteric tract of the cartilaginous fish Scyliorhinus stellaris (L.). Histochem J 17:1033–1041

    Google Scholar 

  • Tagliafierro G, Bonini E, Faraldi G, Farina L, Rossi GG (1988) Distribution and ontogeny of VIP-like immunoreactivity in the gastro-entero-pancreatic system of a cartilaginous fish Scyliorhinus stellaris. Cell Tissue Res 253:23–28

    Google Scholar 

  • Tagliafierro G, Rossi GG, Bonini E, Faraldi G, Farina L (1989) Ontogeny and differentiation of regulatory peptide-and serotonin-immunoreactivity in the gastrointestinal tract of an elasmobranch. J Exp Zool [Suppl 2]:165–174

    Google Scholar 

  • Tagliafierro G, Zaccone G, Fasulo S, Faraldi G, Falugi C (1991) Gastrointestinal neuropeptides and serotonin in some ancient fishes. Gen Comp Endocrinol 82:273

    Google Scholar 

  • Tagliafierro G, Faraldi G, Delù M (1993) Immunohistochemical study on the differentiation of the neuroendocrine system in the gut of a cartilaginous fish. Anim Biol 2:139

    Google Scholar 

  • Thorndyke MC, Falkmer S (1985) Evolution of gastro-entero-pan-creatic endocrine system in lower vertebrates. In: Foreman RE, Gorbman A, Dodd JM, Olsson R (eds) Evolutionary biology of primitive fishes. Plenum Press, New York, pp 371–400

    Google Scholar 

  • Thorndyke MC, Purvis D, Plisetskaya EM (1989) Insulin-like immunoreactivity in the brain of two hagfishes, Heptatretus stouti and Myxine glutinosa. Gen Comp Endocrinol 76:371–381

    Google Scholar 

  • Uvnas-Wallensten K (1977) Occurrence of gastrin in gastric juice, in antral secretion, and in antral perfusates of cats. Gastroenterology 73:487–491

    Google Scholar 

  • Vigna SR (1985) Functional evolution of gastrointestinal hormones. In: Foreman RE, Gorbman A, Dodd JM, Olsson R (eds) Evolutionary biology of primitive fishes. Plenum Press, New York, pp 401–412

    Google Scholar 

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Authors and Affiliations

  1. Istituto di Anatomia Comparata, Università di Genova, Viale Benedetto XV, I-5-16132, Genova, Italy

    Grazia Tagliafierro, Gabriella Faraldi, Mariangela Delù & M. Alessandra Morescalchi

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  1. Grazia Tagliafierro
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  2. Gabriella Faraldi
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  3. Mariangela Delù
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  4. M. Alessandra Morescalchi
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Additional information

A preliminary account of this work was presented to the VII International Ichthyology Congress, Den Haag, 26–30 August 1991

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Tagliafierro, G., Faraldi, G., Delù, M. et al. Gut regulatory peptides in some Antarctic notothenioids. Polar Biol 15, 429–435 (1995). https://doi.org/10.1007/BF00239720

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  • DOI: https://doi.org/10.1007/BF00239720

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