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A protoplast to plant system for the chrysanthemum Dendranthema zawadskii x D. grandiflora

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Summary

Plantlets were regenerated from protoplasts of in vitro shoot cultures and leaf-derived de novo shoots of the chrysanthemum Dendranthema zawadskii x D. grandiflora. Isolated protoplasts reformed cell walls and then began to divide within 24 hours of culture in streaky plate agarose lenses surrounded by liquid V-KM medium. Twenty one days after isolation, 1 mm diameter callus clumps were transferred to shoot regeneration medium. After a further 33 days leaves became visible. Elongated shoots were rooted on half strength hormone-free MS medium.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

3-indoleacetic acid

MS:

Murashige and Skoog (1962)

NAA:

1-naphthylacetic acid

Pfr:

Photon fluence rate

V-KM:

Binding and Nehls (1977)

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Author notes

  1. Susan E. Ledger

    Present address: Dept. of Cellular and Molecular Biology, nUniversity of Auckland, Private Bag, Auckland, New Zealand

Authors and Affiliations

  1. MAF Technology, Ministry of Agriculture and Fisheries, Horticultural Research Centre, Private Bag, Levin, New Zealand

    Graeme C. Lindsay & Susan E. Ledger

Authors
  1. Graeme C. Lindsay
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  2. Susan E. Ledger
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Communicated by F. Constabel

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Lindsay, G.C., Ledger, S.E. A protoplast to plant system for the chrysanthemum Dendranthema zawadskii x D. grandiflora . Plant Cell Reports 12, 278–280 (1993). https://doi.org/10.1007/BF00237135

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  • DOI: https://doi.org/10.1007/BF00237135

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