Summary
The pattern of RNA synthesis and its distribution in subcellular fractions of goldfish brain was studied using uridine-5H3 as the precursor. About 14% of the total RNA synthesized was found to be associated with the synaptosomal fraction after a 3 hr labelling time. The sedimentation characteristics of this RNA was compared with that of the nuclear and cytoplasmic components of brain. The results suggest that the synaptosomal RNA is a membrane-bound fraction with distinctive properties.
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References
Bondy, S.C., Roberts, S.: Biochem. J. 109, 533 (1968).
Glassman, E.: (Ed. by E.E. Snell). Ann. Rev. Biochem. 38, 605 (1969).
Balazs, R., Cocks, W.A.: J. Neurochem. 16, 1035 (1967).
Shashoua, V.E.: Proc. nat. Acad. Sci. (Wash.) 68, 2835 (1971).
Whittaker, V.P., Michaelson, I.A., Kirkland, R.J.A.: Biochem. J. 90, 293 (1964).
Autilio, L.A., Appel, S.H., Pettis, P., Gambetti, P.L.: Biochemistry 7, 2615 (1968).
Scherrer, K., Darnell, J.: Biochem. biophys. Res. Commun. 7, 486 (1962).
Shashoua, V.E.: Proc. nat. Acad. Sci. (Wash.) 65, 160 (1970).
Luft, J.H.: J. biophys. biochem. Cytol. 9, 409 (1969).
Reynolds, E.S.: J. Cell Biol. 17, 208 (1963).
Whittaker, V.P., Greengard, P.: J. Neurochem. 18, 173 (1971).
Bray, G.A.: Analyt. Biochem. 1, 279 (1960).
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This research was supported by grants from NIH (NS09407) and the Grant Foundation.
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Shashoua, V.E. The pattern of synthesis and distribution of membrane bound RNA in brain synaptosomes. Exp Brain Res 17, 139–143 (1973). https://doi.org/10.1007/BF00235023
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DOI: https://doi.org/10.1007/BF00235023