Summary
A method is described for multiple shoot and plantlet formation from zygotic embryos of Taxus brevifolia. Adventitious bud primordia were best induced by culturing zygotic embryos on 1/2B5 medium supplemented with 10 μM BA for 14 days. Further vegetative buds were produced following subculture to half-strength McCown's basal salt medium containing 1.0% activated charcoal. Individual adventitious shoots were excised and approximately 5% of these formed roots. Rooting frequency was increased to 58% by a single treatment with ABT rooting powder. Vigorous growing Taxus brevifolia plants were established after transfer to plant growth medium.
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Communicated by J.M. Widholm
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Chee, P.P. Organogenesis in Taxus brevifolia tissue cultures. Plant Cell Reports 14, 560–565 (1995). https://doi.org/10.1007/BF00231938
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DOI: https://doi.org/10.1007/BF00231938