Abstract
A multifactorial quantitative analysis of oscillations in glycolysis was conducted in the postmicrosomal supernatant of rat muscle homogenates incubated in the presence of yeast hexokinase. Oscillations in adenine nucleotides, D-fructose 1,6-bisphosphate, triose phosphates, L-glycerol 3-phosphate, 3HOH generation from D-[5-3H]glucose, NADH and L-lactate production were documented. The occurrence of such oscillations were found to depend mainly on the balance between the consumption of ATP associated with the phosphorylation of D-glucose, as catalyzed by both yeast and muscle hexokinase, and the net production of ATP resulting from the further catabolism of D-fructose 6-phosphate, as initiated by activation of phosphofructokinase. The oscillatory pattern was suppressed in the presence of D-fructose 2,6-bisphosphate. It is proposed that the quantitative information gathered in this study may set the scene for further studies in extracts of cells other than myocytes, e. g. hepatocytes and pancreatic islet cells, in which no oscillation of glycolysis was so far observed.
Similar content being viewed by others
References
Chance B, Hess B, Betz A: DPNH oscillations in a cell-free extract of S. Carlsbergensis. Biochem Biophys Res Commun 16: 182–187, 1964
Frenkel R: DPNH oscillations in glycolyzing cell free extracts from beef heart. Biochem Biophys Res Commun 21: 497–502, 1965
Pye K, Chance B: Sustained sinusoidal oscillations of reduced pyridine nucleotide in a cell-free extract of Saccharomyces earlsbergensis. Proc. Natl Acad Sci USA 55: 888–894, 1966
Tornheim K, Lowenstein JM: The purine nucleotide cycle. The production of ammonia from aspartate by extracts of rat skeletal muscle. J Biol Chem 247: 162–169, 1972
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951
Berridge MJ, Dawson RMC, Downes CP, Heslop JP, Irvine RF: Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides. Biochem J 212: 473–482, 1983
Stanley PE, Williams SE: Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme. Anal Biochem 29: 381–392, 1969
Bergmeyer HS: Methods in Enzymatic Analysis. Academic Press, New York, 1974, pp 473
Tornheim K, Lowenstein JM: The purine nucleotide cycle. IV. Interactions with oscillations of the glycolytic pathway in muscle extracts. J Biol Chem 249: 3241–3247, 1974
Tornheim K: Fructose 2,6-bisphosphate and glycolytic oscillations in skeletal muscle extracts. J Biol Chem 263: 2619–2624, 1988
Tornheim K, Lowenstein JM: The purine nucleotide cycle. Control of phosphofructokinase and glycolytic oscillations in muscle extracts. J Biol Chem 250: 6304–6314, 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Marynissen, G., Sener, A. & Malaisse, W.J. Oscillations in glycolysis: multifactorial quantitative analysis in muscle extract. Mol Cell Biochem 113, 105–121 (1992). https://doi.org/10.1007/BF00231530
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00231530