Abstract
Experiments in S49 mouse lymphoma cells indicate that adenylate cyclase activity is increased following swelling in hypotonic medium through a mechanism independent of the G-proteins which are involved in hormonal regulation of the enzyme. An intact actin cytoskeleton is apparently required for stimulation of adenylate cyclase by mechanical forces. It was hypothesized that this increase in cAMP may be involved in triggering subsequent volume regulatory events. Manipulation of intracellular CAMP content and protein kinase A activity in S49 cells prior to swelling or during the regulatory volume decrease following swelling provided no evidence of a significant role for CAMP in regulating the extent of initial volume increase or the subsequent regulatory volume decrease. Treatment of S49 cells with 10-200 µM miconazole, previously shown to inhibit adenylate cyclase activity, attenuated the initial volume increase with medium dilution and accelerated the rate of regulatory decrease in a dose-dependent and time-dependent manner. However, incubation with 100 µM miconazole for 20 min, which completely inhibited swelling-induced increases in cAMP content, had no significant effect on either the initial volume expansion or the extent of regulatory volume decrease.
Similar content being viewed by others
References
Gilman AG: G-proteins: transducers of receptor generated signals. Annu Rev Biochem 56: 615–649, 1987
Xenophontos XP, Watson PA, Chua BHL, Haneda T, Morgan HE: Increased CAMP content accelerates protein synthesis in rat heart. Circ Res 65: 647–656, 1989
Watson PA, Haneda T, Morgan HE: Effect of higher aortic pressure on ribosome formation and cAMP content in rat heart. Am J Physiol 256: C1257-C1261, 1989
Haneda T, Watson PA, Morgan HE: Elevated aortic pressure preferentially accelerates ribosome formation through a CAMP-dependent mechanism. In: Alan R. Liss: Molecular Biology of the Cardiovascular System. Inc 1990, pp 21–28
Russo LA, Rannels SR, Laslow KS, Rannels DE: Stretch-related changes in lung cAMP after partial pneumonectomy. Am J Physiol 257: E261-E268, 1989
Watson PA: Accumulation of CAMP and calcium in S49 mouse lymphoma cells following hyposmotic swelling. J Biol Chem 264: 14735–14740, 1989
Morgan HE, Xenophontos XP, Haneda T, McGlaughlin S, Watson PA: Stretch-anabolism transduction. J Appl Cardiol 4: 415–422, 1989
Watson PA: Direct stimulation of adenylate cyclase by mechanical forces in S49 mouse lymphoma cells during hyposmotic swelling. J Biol Chem 265: 6569–6575, 1990
London RD, Lipkowitz MS, Sinert RH, Abramson RG: Modulation of ionic permeability in a nonpolarized cell: effect of cAMP. Am J Physiol 257: F985-F993, 1989
Stalla GK, Stalla J, von Weider K, Muller OA, Gerzer R, Hollt V, Jakobs KH: Nitroimidazole derivatives inhibit anterior pituitary cell function apparently by a direct effect on the catalytic subunit of the adenylate cyclase holoenzyme. Endocrinology 125: 699–706, 1989
Gilman AG: A protein binding assay for adenosine 3′: 5'-cyclic monophosphate. Proc Nat Acad Sci USA 67: 305–312, 1970
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275, 1951
Goldspink DF: The influence of passive stretch on the growth and protein turnover of the denervated extensor digitorum longus muscle. Biochem J 174: 595–602, 1978
Grinstein SA, Rothstein B, Sarkadi B, Gelfand EW: Responses of lymphocytes to anisotonic media: volume-regulating behavior. Am J Physiol 246: C204–215, 1984
Ingber DE, Folkman J: How does extracellular matrix control capillary morphogenesis? Cell 58: 803–805, 1989
Schwartz MA, Both G, Lechene CP: pH regulation in spread cells and round cells. J Biol Chem 265: 1327–1332, 1990
Ingber DE, Prusty D, Frangioni JV, Cragoe EJ, Lechene C, Schwartz MA: Control of intracellular pH and growth by fibronectin in capillary endothelial cells. J Cell Biol 110: 1803–1811, 1990
Sachs F: Baroreceptor mechanisms at the cellular level. Fed Proc 46: 12–16, 1987
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Watson, P.A., Giger, K.E. & Frankenfield, C.M. Activation of adenylate cyclase during swelling of S49 cells in hypotonic medium is not involved in subsequent volume regulation. Mol Cell Biochem 104, 51–56 (1991). https://doi.org/10.1007/BF00229803
Issue Date:
DOI: https://doi.org/10.1007/BF00229803