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A Method to Simultaneously Detect Changes in Intracellular Ca2+ Concentration and Cell Volume

  • Cell Biophysics
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Abstract

A method to simultaneously assess the changes in intracellular calcium concentration and cell volume in single cells was developed using the Ca2+-sensitive fluorescent probe Fura-2 and a three-dimensional image-surface reconstruction technique, respectively. Studies with this method showed that Fura-2 loading had no significant effect on the kinetics of A549 human epithelial cell swelling in a hypotonic solution, as well as the volume restoration kinetics. Significant changes in intracellular Ca2+ concentration were not observed in the examined volume modulation range. The results suggest that Ca2+-mediated signaling pathways are not involved in the autoregulation of the cell volume in A549 cells exposed to hypotonic conditions.

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Abbreviations

RVD:

regulatory volume decrease

DISUR:

double image surface reconstruction technique

References

  1. N. A. McCarty and R. G. O’Neil, Physiol. Rev. 72, 1037 (1992).

    Article  Google Scholar 

  2. J. Altamirano, M. S. Brodwick, and F. J. Alvarez-Leefmans, J. Gen. Physiol. 112, 145 (1998).

    Article  Google Scholar 

  3. F. Boudreault and R. Grygorczyk, J. Physiol. 561, 499 (2004).

    Article  Google Scholar 

  4. M. R. Shen, P. Furla, C. Y. Chou, et al., Pflugers Arch. 444, 276 (2002).

    Article  Google Scholar 

  5. E. R. O’Connor and H. K. Kimelberg, J. Neurosci. 13, 2638 (1993).

    Article  Google Scholar 

  6. S. F. Pedersen, J. Prenen, G. Droogmans, et al., J. Membr. Biol. 163, 97 (1998).

    Article  Google Scholar 

  7. H. Tinel, E. Kinne-Saffran, and R. K. Kinne, Cell Physiol. Biochem. 10, 297 (2000).

    Article  Google Scholar 

  8. N. K. Jorgensen, S. Christensen, H. Harbak, et al., J. Membr. Biol. 157, 281 (1997).

    Article  Google Scholar 

  9. P. Doroshenko and E. Neher, J. Physiol. 449, 197 (1992).

    Article  Google Scholar 

  10. T. Speake, I. J. Douglas, and P. D. Brown, J. Membr. Biol. 164, 283 (1998).

    Article  Google Scholar 

  11. J. S. Adorante and P. M. Cala, Am. J. Physiol. 268, C721 (1995).

    Article  Google Scholar 

  12. B. J. Lippmann, R. Yang, D. W. Barnett, and S. Misler, Brain Res. 686, 29 (1995).

    Article  Google Scholar 

  13. H. Pasantes-Morales, T. E. Maar, and J. Moran, J. Neurosci. Res. 34, 219 (1993).

    Article  Google Scholar 

  14. H. Pasantes-Morales, R. A. Murray, L. Lilja, and J. Moran, Am. J. Physiol. 266, C165 (1994).

    Article  Google Scholar 

  15. R. Sanchez-Olea, M. Morales Mulia, J. Moran, and H. Pasantes-Morales, Neurosci. Lett. 193, 165 (1995).

    Article  Google Scholar 

  16. S. N. Orlov, S. L. Aksentsev, and S. V. Kotelevtsev, Cell Calcium 38, 53 (2005).

    Article  Google Scholar 

  17. S. V. Koltsova, J. Tremblay, P. Hamet, and S. N. Orlov, Cell Calcium 58, 317 (2015).

    Article  Google Scholar 

  18. F. Boudreault and R. Grygorczyk, J. Microsc. 215, 302 (2004).

    Article  MathSciNet  Google Scholar 

  19. N. Groulx, F. Boudreault, S. N. Orlov, and R. Grygorczyk, J. Membr. Biol. 214, 43 (2006).

    Article  Google Scholar 

  20. S. Tatur, N. Groulx, S. N. Orlov, and R. Grygorczyk, J. Physiol. 584, 419 (2007).

    Article  Google Scholar 

  21. E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen, Physiol. Rev. 89, 193 (2009).

    Article  Google Scholar 

  22. J. Fels, S. N. Orlov, and R. Grygorczyk, Biophys. J. 96, 4276 (2009).

    Article  ADS  Google Scholar 

  23. A. Platonova, F. Boudreault, L. V. Kapilevich, et al., J. Membr. Biol. 247, 571 (2014).

    Article  Google Scholar 

  24. A. Platonova, O. Ponomarchuk, F. Boudreault, et al., Biochim. Biophys. Acta 1848, 2337 (2015).

    Article  Google Scholar 

  25. K. J. Bibby and A. G. McCulloch, Am. J. Physiol. 266, C1639 (1994).

    Article  Google Scholar 

  26. R. Liu, and A. E. Persson, J. Physiol. 563, 895 (2005).

    Article  Google Scholar 

  27. R. Thul, S. Coombes, H. L. Roderick, and M. D. Bootman, Proc. Natl. Acad. Sci. U. S. A. 109, 2150 (2012).

    Article  ADS  Google Scholar 

  28. O. Ponomarchuk, F. Boudreault, S. N. Orlov, and R. Grygorczyk, Pflugers Arch.–Eur. J. Physiol. 468, 2075 (2017).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biology, Moscow State University, Moscow, 119991, Russia

    O. O. Ponomarchuk, A. A. Shiyan, G. V. Maksimov & S. N. Orlov

  2. Centre de Recherche, Centre Hospitalier de l’Université de Montréal, Tour Viger, rue St-Denis 900, Quebec H2X 0A9, Montréal, Canada

    O. O. Ponomarchuk, F. Boudreault, A. A. Shiyan & R. Grygorczyk

  3. Siberian State Medical University, Moskovskii trakt 2, Tomsk, 634050, Russia

    S. N. Orlov

Authors
  1. O. O. Ponomarchuk
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  2. F. Boudreault
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  3. A. A. Shiyan
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  4. G. V. Maksimov
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  5. R. Grygorczyk
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  6. S. N. Orlov
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Corresponding author

Correspondence to S. N. Orlov.

Additional information

Original Russian Text © O.O. Ponomarchuk, F. Boudreault, A.A. Shiyan, G.V. Maksimov, R. Grygorczyk, S.N. Orlov, 2018, published in Biofizika, 2018, Vol. 63, No. 3, pp. 489–495.

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Ponomarchuk, O.O., Boudreault, F., Shiyan, A.A. et al. A Method to Simultaneously Detect Changes in Intracellular Ca2+ Concentration and Cell Volume. BIOPHYSICS 63, 369–374 (2018). https://doi.org/10.1134/S000635091803020X

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  • DOI: https://doi.org/10.1134/S000635091803020X

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