Summary
Cultured heart cells have been shown useful for investigating states of oxygen and volume restrictions, simulating anoxia and ischemia-like states at cellular levels. The sarcolemma has been implicated as one of the early sites of ischemic damage; therefore, lactoperoxidase catalyzed radioiodination was used to study accessibility of the sarcolemmal lipid moieties to this enzymatic probe, reflecting their exposure to the extracellular environment, hence the biophysical state of the sarcolemma. These studies have shown that within one hour of ‘ischemic’ injuries: (1) The degree of labelling in the total phospholipid fraction is consideraly increased; and (2) Profound changes in the relative extent of labelling of different phospholipid classes were observed. The PE/PC labelling ratio increases dramatically with the progress of ischemia-like state. We suggest that early during ischemic injury, reorganization of the cell surface phospholipids occurs and discuss possible relations to the energy charge of the cell.
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Hillis LD, Braunwald E: N Engl J Med 296:971–978 (Part I), 1034–1042 (Part II). 1093–1096, (Part II), 1977
Neely JR, Feuvray D: Am J Pathol 102:282–291, 1981
Parmelt WW, Chatterjee K: J Cardiovasc Pharmacol 6:(Suppl. 6), 888–894, 1984
Pantely GA, Bristow JD: Prog Cardiovasc Dis 27:95–114, 1984
Acosta D, Puckett M, McMillan R: In Vitro 14:728–732, 1978
Acosta D, Puckett M, McMillin R: Experientia 34:1338–1339, 1978
Higgins TJC, Allsopp D, Bailey PJ: J Mol Cell Cardiol 12:909–927, 1980
Van der Laarse A, Hollaar L, Van den Valk LJM, Witteveen SAGJ: J Mol Med 3:123–131, 1978
Wenzel DG, Acosta D: Res Common Chem Pathol Pharmacol 12:173–176, 1975
Higgins TC, Allsopp D, Bailey PJ, D'Souza EDA: J Mol Cell Cardiol 13:599–611, 1981
Vemuri R, Yagev S, Heller M, Pinson A: In Vitro 21:521–525, 1985
Vemuri R, Heller M, Pinson A: Basic Res Cardiol 80 (Suppl. 2):140–144, 1985
Zimmerman ARE, Dames W, Hülsmann WD, Snidjeder J, Wisse E, Durrer D: Cardiovasc Res 1:210–209, 1967
Jennings RJ, Reimer KA: Am J Pathol 102:241–245, 1981
Hess ML, Krausse S, Kontos HA: Adv Exptl Med Biol. 161:3777–3779, 1983
Benenson A, Mersel M, Heller M, Pinson A. In: Dhalla NM (ed) Methods in Studying Cardiac Membranes CRC Press Inc., Boca Raton, Vol. II, 1984, pp 59–61
Benenson, A, Mersel M, Pinson A, Heller M: Anal Biochem, 101:507–512, 1980
Mersel M, Benenson A, Pinson A, Heller M: FEBS Lett, 110:69–72, 1980
Benenson A, Heller M, Pinson A, Mersel M: In: Calderera CM, and Harris P (eds) advances in Studies on Heart Metabolism CLUEB, Bologna, Italy, 1982, pp 49–56
Gross Z, Rottem S: Biochim Biophys Acta 555:547–542, 1979
Schlager SI: Methods in Enzymol 70:252–265, 1980
Morisson M, Schonbaum GR: Annu Rev Biochem 45: 861–888, 1976
Monteralo RC, Rueckert RR: Archiv Biochem Phys 178:555–564, 1970
Yagev S, Heller M, Pinson A: In Vitro, 20:893–898, 1984
Heller M, Harary I: Biochim Biophys Acta 467:29–43, 1977
Mersel M, Malvina AN, Hindelang C, Mandel P: Biochim Biophys Acta, 778:144–154, 1984
Dhalla NS, Pierce GN. In: Dhalla NS (ed) Methods in Studying Cardiac Membranes CRC Press, Boca Raton, Fl. Vol I, 1984, pp 3–18
Rouser D, Kritschevski G, Yamamoto A. In: Marinetti GV (ed) Lipid Chromatography Analysis M. Dekker, New York 1967, pp 99
Burton LM, Hagler HK, Templeton GH, Willerson J, Buja LMJ: J Clin Investig 60:1289–1302, 1977
Burton LM, Hagler HK, Willerson JT, Buja LM: Am J Physiol 241:H714-H723, 1981
Burton LM, Templeton GHJ, Hagler HK, Willerson JT, Buja LM: J Mol Cell Cardiol 12: 109–133, 1980
Chien KR, Reeves JP, Buja LM, Bonte F, Parkey RW Willerson JT Cir Res 48:711–719, 1981
Langer GA, Frank JA, Brady AJ: Int Rev Physio 9:191–237, 1976
Gould BJ, Ginsberg BH. In: Allan R (ed) Membrane, Detergents and Receptor Solubilization Liss Inc. New York, 1984, pp 65–83
Dessort D, Mersel M, Lepage P, Van Doersaller A: Anal Biochem 142:43–52, 1984
Dominski J, Binaglia L, Dreyfus H, Massarelli R, Mersel M, Freysz L: Biochim Biophys Acta 734:257–266, 1983
Mersel M, Benenson A, Mandel P, Freysz L. In: Phospholipids in the Nervous System Vol 1: Metabolism (Horroks GB, Ansell GB and Porcellati G, eds) Raven Press, New York, 1982, p 329
Vemuri R, Huizer T, De Jong JW, Hegge JAJ, Heller M, Pinson A: (submitted)
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Vemuril, R., Mersel, M., Heller, M. et al. Studies on oxygen and volume restriction in cultured cardiac cell: possible rearrangement of sarcolemmal lipid moieties during anoxia and ischemia-like states. Mol Cell Biochem 79, 39–46 (1988). https://doi.org/10.1007/BF00229396
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DOI: https://doi.org/10.1007/BF00229396