Summary
In the pars distalis of the hypophysis of adult Rana temporaria, three types of nerve-fiber profiles were found at two distinct sites, in both lateral parts of the bordering regions of the anterior lobe with the intermediate lobe of the hypophysis. The first type of nerve-fiber profile consists of bundles of very fine axonal elements (diameter: <0.7 μm). The second type is formed by larger nerve fibers (diameter up to 4 μm) containing a few neurosecretory granules of approximately 100 nm. The third type of nervefiber profile resembles the second type but these nerve fibers make synaptoid contacts on at least two different types of glandular cells. The possible functional significance of these nerve fibers in the pars distalis is discussed.
No nerve fibers were found (1) in the central part of the bordering region of the pars distalis with the intermediate lobe, (2) at the bordering region with the median eminence and (3) with the neurohypophysial stalk, and (4) in all other parts of the pars distalis.
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References
Aronsson S (1976) The ontogenesis of monoaminergic nerve fibers in the hypophysis of Rana temporaria with special reference to the pars distalis. Cell Tissue Res 171:437–448
Bartels W (1971) Ontogenese der aminhaltigen Neuronensysteme im Gehirn von Rana temporaria. Z Zellforsch 116:94–118
Dierickx K, Goossens N, Vandenberghe MP (1973) Identification of adenohypophysiotropic neurohormone producing neurosecretory cells in Rana temporaria. III. The tubero-hypophysial monoaminergic fibers and the role of the tubero-hypophysial neurosecretory system. Z Zellforsch 143:93–106
Doerr-Schott J (1974) Localisation submicroscopique par cytoimmunoenzymologie de différents principes hormonaux de l'hypophyse de Rana temporaria L. J Microsc 20:151–164
Enemar A, Falck B (1965) On the presence of adrenergic nerves in the pars intermedia of the frog, Rana temporaria. Gen Comp Endocrinol 5:577–583
Green JD (1966) The comparative anatomy of the portal vascular system and the innervation of the hypophysis. In: Harris GW, Donovan BT (eds) The pituitary gland, Vol I. Butterworths, London
Harris GW (1971) Humours and hormones. Proc Soc Endocrinol, J Endocrinol 53
Holmes RL, Ball JN (1974) The pituitary gland. A comparative account. Cambridge University Press, London
Kurosumi K, Kobayashi Y (1980) Nerve fibers and terminals in the rat anterior pituitary gland as revealed by electron microscopy. Arch Histol Jpn 43:141–155
Metuzals J (1954) Neurohistologische Studien über die nervöse Verbindung der Pars distalis der Hypophyse mit dem Hypothalamus auf dem Wege des Hypophysenstieles. Acta Anat 20:258–285
Metuzals J (1955) Die Innervation der Drüsenzellen der Pars distalis der Hypophyse bei der Ente. (Mit Vergleich zwischen nervösem Endplexus und argyrophilem Bindegewebe). Z Zellforsch 43:319–334
Metuzals J (1956) The innervation of the adenohypophysis in the duck. J Endocrinol 14:87–95
Mira-Moser F (1972) L'ultrastructure de l'adénohypophyse du crapaud Bufo bufo L. III. Différenciation des cellules de la pars distalis au cours du développement larvaire. Z Zellforsch 125:88–107
Nakane PK (1970) Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J Histochem Cytochem 18:9–20
Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212
Unsicker K (1971) On the innervation of mammalian endocrine glands (anterior pituitary and parathyroids). Z Zellforsch 121:283–291
Vandesande F, Goossens N (1973a) Ein halbautomatisches Mehrzweckgerät für die quantitative Bildanalyse. Leitz-Mitt Wiss u Techn 6:65–67
Vandesande F, Goossens N (1973b) Een semi-automatisch toestel voor de stereologie. Natuurwet Tijdschr 55:244–251
Westlund KN, Childs GV (1982) Localization of serotonin fibers in the adenohypophysis. Endocrinology 111:1761–1763
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Van Vossel, A., Van Vossel-Daeninck, J. Electron-microscopic investigation of the innervation of the pars distalis of the hypophysis in adult Rana temporaria L.. Cell Tissue Res. 237, 149–154 (1984). https://doi.org/10.1007/BF00229210
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DOI: https://doi.org/10.1007/BF00229210