Summary
Studies were conducted to establish the relationship between deoxyguanosine kinase activity and human cytomegalovirus (HCMV) infection. Using both PAGE and isoelectric focusing techniques, extracts from untreated and infected cells were examined for deoxyguanosine kinase activity. The analyses resulted in identical migration rates for deoxyguanosine kinase activity in both infected and uninfected extracts. These data and kinetic studies based on apparent Km values suggest that HCMV enhanced a cellular kinase activity rather than coded for a virus specific enzyme. Furthermore, our results indicated that infected cells, like normal fibroblasts, contain two deoxyguanosine kinase activities, one of mitochondrial and another of cytosolic origin. Of particular interest was the observation that HCMV infection caused an enhancement of the mitochondrial enzymatic activity while the cytosolic activity showed no change. Deoxycytidine kinase activity which is associated with cytosolic deoxyguanosine kinase was unaffected by HCMV infection.
Similar content being viewed by others
References
St. Jeor S, Albrecht TB, Funk FD, Rapp F: Stimulation of Cellular DNA Synthesis by Human Cytomegalovirus. J Virol 13:353–362, 1974.
Tanaka S, Furukawa T, Plotkin SA: Human Cytomegalovirus Stimulates Host RNA Synthesis. J Virol 15:297–304, 1975.
Hirai K, Watanabe Y: Induction of Alpha-type DNA Polymerases in Human Cytomega lovirus-infected WI 38 Cells. Biochim Biophys Acta 447:328–339, 1976.
Tanaka S, Ihara S, Watanabe Y: Human Cytomegalovirus Induces DNA-dependent RNA-polymerases in Human Diploid Cells. Virology 89:179–185, 1978.
Zavada V, Erban V, Rezacova D, Vonka V: Thymidine-kinase in Cytomegalovirus Infected Cells. Arch Virol 52: 333–339, 1976.
Durham JP, Ives DH: Deoxycytidine Kinase, II Purification and General Properties of the Calf Thymus Enzyme. J Biol Chem 245:2276–2284, 1970.
Gower WR, Jr., Carr MC, and Ives DH: Deoxyguanosine Kinase. Distinct Molecular Forms in Mitochondria and Cytosol. J Biol Chem 254:2180–2183, 1979.
Tocci MJ and St. Jeor S: Persistence and Replication of the Human Cytomegalovirus Genome in Lymphoblastoid Cells of B and T Origin. Virology 96:664–668, 1979.
Schneider EL, Stanbridge EJ, Epstein CJ: Incorporation of 3H-Uridine and 3H-Uracil into RNA. A Simple Technique for the Detection of Mycoplasma Contamination of Cultured Cell. Exptl Cell Res 84:311–318, 1974.
Green FJ, Lewis RA: Partial Purification and Characterization of Deoxyguanosine Kinase from Pig Skin. Biochem J 183:547–553, 1979.
Phillips RC, George P, Rutman RJ: Thermodynamic Studies of the Formation and Ionization of the Magnesium (II) Complexes of ADP and ATP over the pH Range 5 to 9. J Am Chem Soc 88:2631–2640, 1966.
Barker J, Lewis RA: Deoxyguanosine Kinase of Neonatal Mouse Skin Tissue. Biochim Biophys Acta 658:111–123, 1980.
Radola BJ: Isoelectric Focusing in Layers of Granulated Gels 1. Thin-Layer Isoelectric Focusing of Proteins. Biochim Biophys Acta 295:412–428, 1973.
Chappell J, Hansford RG: Preparation of Mitochondria From Animal Tissues and Yeasts. In GD Birnie (ed.) Subcellular Components, Preparation and Fractionation, 2nd Ed., Butterworths, London, pp 77–91, 1972.
Jerkofsky MA, Dobersen MJ, Greer S: Inhibition of the Replication of Human Cytomegalovirus by Bromodeoxycytidine in the Absence of Detectable Increases in Bromodeoxycytidine Kinase Activity. Invervirol 14:233–238, 1980.
Furukawa T, Sakuma S, Plotkin SA: Human Cytomegalovirus Infection of WI-38 Cells Stimulates Mitochondrial DNA Synthesis. Nature 262:414–416, 1976.
Furukawa T, Tanaka S, Plotkin SA: Stimulation of Macromolecular Synthesis in Guinea Pig Cells by Human CMV. Proc Soc Exp Biol Med 148:211–214, 1975.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lewis, R.A., Watkins, L. & St. Jeor, S. Enhancement of deoxyguanosine kinase activity in human lung fibroblast cells infected with human cytomegalovirus. Mol Cell Biochem 65, 67–71 (1984). https://doi.org/10.1007/BF00226020
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00226020