Abstract
We studied the relationship between membrane potential and respiration rate in isolated liver mitochondria from rats fed an energy dense diet. We conceptually divided the system into blocks of reactions that produced or consumed mitochondrial membrane potential and then measured the kinetic response of these blocks of reactions to this potential using NAD-linked and FAD-linked substrates. We show that decreased respiration rate with an NAD-linked substrate is accounted for by decreased kinetic response of the substrate oxidation pathway to the potential. No variation in the kinetic response of the above blocks of reactions to the potential was found using an FAD-linked substrate. These results indicate that FAD-linked and NAD-linked pathways are differently affected in rats fed an energy dense diet.
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Liverini G, Iossa S, Barletta A: Hepatic mitochondrial respiratory capacity in hyperphagic rats. Nutr Res 14: 1671–1682, 1994
Iossa S, Mollica MP, Lionetti L, Barletta A, Liverini G: Hepatic mitochondrial respiration and transport of reducing equivalents in rats fed an energy dense diet. Int J Obes 19: 539–543, 1995
Liverini G, Iossa S, Lionetti L, Mollica MP, Barletta A: Sympathetically-mediated thermogenic response to food in rats. Int J Obes 19: 87–91, 1995
Rothwell NJ, Stock MJ: A role for brown adipose tissue in diet-induced thermogenesis. Nature 281: 31–35, 1979
Rothwell NJ, Stock MJ: Brown adipose tissue in diet-induced thermogenesis. In: P. Trayhum and D.G. Nicholls (eds). Brown Adipose Tissue. Edward Arnold, London, 1986, pp 269–286
Berry MN, Clark DG, Grivell AR, Wallace PG: The contribution of hepatic metabolism to diet induced thermogenesis. Metabolism 34: 141–147, 1985
Ma SWY, Nadeu BA, Foster DO: Evidence for liver as the major site of the diet-induced thermogenesis of rats fed a ‘cafeteria’ diet. Can J Physiol Pharmacol 65: 1802–1804, 1987
Ma SWY, Foster DO: Brown adipose tissue, liver, and diet-induced thermogenesis in cafeteria diet-fed rats. Can J Physiol Pharmacol 67: 367–381, 1989
Hansford RG: Bioenergetics in aging. Biochim Biophys Acta 726: 41–80, 1983
La Noue KF, Schoolwerth AC: Metabolite transport in mitochondria. Annu Rev Biochem 48: 871–922, 1979
Nicholls DG: Bioenergetics, an Introduction to the Chemiosmotic Theory. Academic Press, London, 1982
Brand MD: The proton leak across the mitochondrial inner membrane. Biochim Biophys Acta 1018: 128–133, 1990
Harper ME, Ballantyne JS, Leach M, Brand MD: Effects of thyroid hormones on oxidative phosphorylation. Bioch Soc Trans 21: 785–792, 1993
Hafner RP, Nobes CD, McGown AD, Brand MD: Altered relationship between protonmotive force and respiration rate in non-phosphorylating liver mitochondria isolated from rats of different thyroid hormone status. Eur J Biochem 178: 511–518, 1988
Hafner RP, Leake MJ, Brand MD: Hypothyroidism in rats decreases mitochondrial inner membrane cation permeability. FEBS Lett 248: 175–178, 1989
Hafner RP, Brown GC, Brand MD: Thyroid-hormone control of state respiration in isolated rat liver mitochondria. Biochem J 265: 731–734, 1990
Brand MD, D'Alessandri L, Reis HMGPV and Hafner RP: Stimulation of the electron transport chain in mitochondria isolated from rats treated with mannoheptulose or glucagon. Arch Biochem Biophys 283: 278–284, 1990
Iossa S, Lionetti L, Mollica MP, Barletta A and Liverini G: Thermic effect of food in hypothyroid rats. J Endocrinol 148: 167–174, 1996
Barr HG, McCracken KJ: High efficiency of energy utilization in ‘cafeteria’ and force-fed rats kept at 29°C. Br J Nutr 51: 379–387, 1984
Hartree EF: Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem 48: 422–427, 1972
Brown GC, Brand MD: Thermodynamic control of electron flux through mitochondrial cytochrome bc1 complex, Biochem J 225: 399–405, 1985
Chen RF: Removal of fatty acid from serum albumin by charcoal treatment. J Biol Chem 242: 173–181, 1967
Brand MD, Harper ME, Taylor HC: Control of the effective P/O ratio of oxidative phosphorylation in liver mitochondria and hepatocytes. Biochem J 291: 739–748, 1993
Brown GC, Hafner RP, Brand MD: A ‘top-down’ approach to the determination of control coefficients in metabolic control theory. Eur J Biochem 188: 321–323, 1990
Naim M, Brand JG, Kate MR, Carpenter RG: Energy intake, weight gain and fat deposition in rats fed flavored nutritionally controlled diets in a multichoice (‘cafeteria’) design. J Nutr 115: 1447–1458, 1985
Allard M, LeBland J: Effect of cold acclimation, cold exposure and palatability on postprandial thermogenesis in rats. Int J Obes 12: 169–178, 1988
Brady LJ, Hopper CL: Effect of diet and starvation on hepatic mitochondrial function in the rat. J Nutr 113: 2129–2137, 1983
Rothwell NJ, Stock MJ, Warwick BP: Energy balance and brown fat activity in rats fed cafeteria diet or high-fat, semisynthetic diets at several levels of intake. Metab 34: 474–480, 1985
Brady PS, Knoeber CM, Brady LJ: Hepatic mitochondrial and peroxisomal oxidative capacity in riboflavin deficiency: effect of age, dietary fat and starvation. J Nutr 116: 1992–1999, 1986
Mazier MJP, LeBland J: How dietary fat intake affects cold tolerance and energy balance in the rat. Nutr Res 11: 807–818, 1991
Murphy MP: Slip and leak in mitochondrial oxidative phosphorylation. Biochim Biophys Acta 977: 123–141, 1989
Seifter S, England S: Energy Metabolism. In: I.M. Arias, B. Jakobi, H. Popper, D. Schachter, D.A. Schafritz (eds). The liver: Biology and Pathobiology. Raven Press: New York, 1988, pp 279–315
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ionetti, L., Iossa, S., Brand, M.D. et al. Relationship between membrane potential and respiration rate in isolated liver mitochondria from rats fed an energy dense diet. Mol Cell Biochem 158, 133–138 (1996). https://doi.org/10.1007/BF00225839
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DOI: https://doi.org/10.1007/BF00225839