Abstract
The sequence homology of co-migrating RAPD markers within a genus, across species, and among populations of a species was investigated. DNA was isolated from ten wild Brassica species with n=9 and the RAPD patterns were established using three random primers. Five RAPD markers which appeared to be characteristic for the n=9 species (genus level), four markers which appeared to be species specific, and one population-specific marker were isolated from agarose gels and hybridized to the RAPD profiles of the ten Brassica species. Two RAPD markers were cloned for comparison with gel-isolated RAPD fragment probes in hybridization experiments. Non-specific and background hybridization, occurring when gel-isolated fragments were used as probes, disappeared when cloned fragments were used. A total of 250 RAPD-marker hybridizations were scored according to visual presence or absence in a gel lane. All except three markers hybridized as expected, resulting in an error rate of 1.2%. The deviating results included a lack of hybridization although a band was visible in the gel, a length polymorphism for one marker, and a dual hybridization signal for two single-band markers.
Similar content being viewed by others
References
Cognato AI, Rogers SO, Teale SA (1995) Species diagnosis and phylogeny of Ips grandicollis group (Coleoptera: Scolytidae) using random amplified polymorphic DNA. Ann Entomol Soc Amer 88:397–405
Devos KM, Gale MD (1992) The use of random amplified polymorphic markers in wheat. Theor Appl Genet 84:567–572
Edwards K, Johnstone C, Thompson C (1991) A simple and random method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349
Echt CS, Erdahl LA, McCoy TJ (1992) Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa. Genome 35:84–87
Gonzalez JM, Ferrer E (1993) Random amplified polymorphic DNA analysis in Hordeum species. Genome 36:1029–1031
Kresovich S, Williams JGK, McFerson JR, Routman EJ, Schaal BA (1992) Chracterization of genetic identities and relationships of Brassica oleracea L. via a random amplified polymorphic DNA assay. Theor Appl Genet 85:190–196
Lannér-Herrera C, Gustaffson M, Fält AS, Bryngelsson T (1996) Diversity in natural populations of wild Brassica oleracea as estimated by isozyme and RAPD analysis. Genet Res Crop Evol 43:13–23
Mailer RJ, Scarth R, Fristensky B (1994) Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphisms amplified from arbitrary primers. Theor Appl Genet 87:697–704
Morgan UM, Constantine CC, Greene WK, Thompson RCA (1993) RAPD (random amplified polymorphic DNA) analysis of Giardia DNA and correlation with isoenzyme data. Trans Roy Soc Trop Med Hyg 87:702–705
Reiter RS, Williams JGK, Feldmann KJ, Rafalski JA, Tingey SC, Scolnik PA (1992) Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymorphic DNAs. Proc Natl Acad Sci USA 89:1477–1488
Santos JB dos, Nienhus J, Skroch P, Tivang J, Slocum MK (1994) Comparison of RAPD and RFLP genetic markers in determining genetic similarity among Brassica oleracea L. genotypes. Theor Appl Genet 87:909–915
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Thorman CE, Osborn TC (1992) Use of RAPD and RFLP markers for germplasm evaluation. In: Applications of RAPD technology to plant breeding. Proceedings, Joint Plant Breeding Symposia Series, 1992, Minneapolis, Minnesota, pp 9–11
Tinker NA, Fortin MG, Mather DE (1993) Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor Appl Genet 85:976–984
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Author information
Authors and Affiliations
Additional information
Communicated by P. M. A. Tigerstedt
Rights and permissions
About this article
Cite this article
Lannér, C., Bryngelsson, T. & Gustafsson, M. Genetic validity of RAPD markers at the intra- and inter-specific level in wild Brassica species with n=9. Theoret. Appl. Genetics 93, 9–14 (1996). https://doi.org/10.1007/BF00225720
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225720