Summary
The dynamics of the “A” cells of the parsintercerebralis of Oncopeltus fasciatus over the first eight days of adult life was studied by microspectrophotometry of sections stained either with aldehyde fuchsin or alcian blue 8 GX. The data show that the two stains differ in their selectivity as they record different events in the history of the cells. A hypothesis is proposed that the aldehyde fuchsin is more sensitive to the presence of a “carrier” protein in the cell, whereas alcian blue 8 GX is more sensitive to the presence of the “active principle” in the cell.
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Adams, C. M. W., Sloper, J. C.: The hypothalamio elaboration of posterior pituitary principles in man, the rat and the dog. Histochemical evidence derived from a performic acidalcian blue reaction for cystine. J. Endocr. 13, 221–228 (1956)
Breslow, E., Aaning, H., Abrash, L., Schmir, M.: Physical and chemical properties of bovine neurophysins. J. biol. Chem. 246, 5179–5188 (1971)
Breslow, E., Walter, R.: Binding properties of bovine neurophysins I and II: An equilibrium study. Molec. Pharmacol. 8, 75–81 (1972)
Burkhardt, H.: Analysis of variance—PDP-5/8. Decus Programme Library, Digital Equipment Corporation, Maynard, Mass. (1964)
Cheng, K. W., Priesen, H. G.: Isolation and characterization of a third component of porcine neurophysin. J. biol. Chem. 246, 7656–7665 (1971)
Cymborowski, B., Dutkowski, A.: Circadian changes in protein synthesis in the neurosecretory cells of the central nervous system of Acheta domesticus. J. Insect Physiol. 16, 341–348 (1970)
Dingle, H.: The relation between age and flight activity in the milkweed bug Oncopeltus. J. exp. Biol. 42, 269–283 (1965)
Doane, W. W.: The role of hormones in insect development. In: Developmental systems: Insects, vol. 2, p. 291–497 (eds. Counce, S. J., Waddington, C. H.). London and New York: Academic Press 1973
Du Vigneuud, V., Bessler, V., Swan, C., Roberts, J., Katsoyannis, P., Gordon, J.: The synthesis of an octapeptide amide with the hormonal activity of oxytocin. J. Amer. chem. Soc. 75, 4879–4880 (1953)
Engelmann, F.: The physiology of insect reproduction. New York: Pergamon Press 1970
Ewen, A. B.: An improved aldehyde fuchsin staining technique for neuroseoretory products in insects. Trans. Amer. micr. Soc. 81, 94–96 (1962)
Gillot, C., Dogra, G. S.: Neurosecretory cell and corpus allatum activity during successive production of egg batches in virgin Melanoplus sanguinipes. Gen. comp. Endocr. 18, 126–132 (1972)
Gordon, H., Bandai, S.: Effect of mating on egg production by the large milkweed bug Oncopeltus fasciatus. Ann. ent. Soc. Amer. 60, 1099–1102 (1967)
Hadler, W., deLueca, O., Ziti, L. M., Patelli, A. S., Vozza, J.: Histochemical meaning of the chromealum hematoxylin and the aldehyde fuchsin staining; an investigation accomplished by a spot test technique carried out on filter paper models. Acta histochem. (Jena) 29, 320–338 (1968)
Highnam, K. C., Mordue, W.: Estimates of neurosecretory activity by an autoradiographic technique in adult female Schistocerca gregaria (Porsk.). Gen. comp. Endocr. 15, 31–38 (1970)
Hollenberg, M. D., Hope, D. B.: The isolation of the native hormone-binding proteins from bovine pituitary posterior lobes. Biochem. J. 106, 557–564 (1968)
Johansson, A. S.: The nervous system of the milkweed bug Oncopeltus fasciatus (Dallas) (Heteroptera-Lygaeidae). Trans. Amer. ent. Soc. 83, 119–183 (1957)
Lehninger, A.: Biochemistry, p. 104. New York: Worth Publishers Inc. 1970
Loher, W., Gordon, H.: The maturation of sexual behaviour in a new strain of the large milkweed bug Oncopeltus fasciatus. Ann. ent. Soc. Amer. 61, 1566–1572 (1968)
Mahon, D. G., Nair, K. K.: Stoichiometry of aldehyde fuchsin staining reaction for proteins. J. Histochem. Cytochem. (1975) in press
Meola, R., Lea, A. O.: Independence of paraldehyde fuchsin staining of the corpus cardiacum and the presence of the neurosecretory hormone required for egg development in the mosquito. Gen. comp. Endocr. 16, 105–111 (1971)
Pearse, A. G. E.: Histochemistry, theoretical and applied, vol. I, p. 707. London: J. A. Churchill Ltd. 1968
Rauch, R., Hollenberg, M. D., Hope, D. B.: Isolation of a third bovine neurophysin. Biochem. J. 115, 473–479 (1969)
Schoumacker, H., van Damme, N.: Maillet's OsO4-ZnI2 fixative and alcian blue staining in the study of neurosecretion; invertebrates. Stain Technol. 46, 233–327 (1971)
Schreiner, B.: Histochemistry of the “A” cell neurosecretory material in the milkweed bug, Oncopeltus fasciatus (Heteroptera: Lygaeidae), with a discussion of the neurosecretory material/carrier substance problem. Gen. comp. Endocr. 6, 388–400 (1966)
Sokal, R., Rohlf, F. J.: Biometry. San Francisco: W. H. Freeman and Co. 1969
Unnithan, G. C., Bern, H. A., Nayar, K. K.: Ultrastructural analysis of the neuroendocrine apparatus of Oncopeltus fasciatus (Heteroptera). Acta zool. 52, 117–143 (1971)
Wigglesworth, V. B.: The hormonal regulation of growth and reproduction in insects. Advanc. Insect. Physiol. 2, 244–332 (1964)
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Supported by National Research Council grant A4669 and E2465.
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Mahon, D.C., Nair, K.K. A comparison of aldehyde fuchsin and alcian blue staining of neurosecretory material in Oncopeltus fasciatus . Cell Tissue Res. 161, 477–484 (1975). https://doi.org/10.1007/BF00224138
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DOI: https://doi.org/10.1007/BF00224138