Skip to main content

Interspecific hybridization between common and tepary beans: increased hybrid embryo growth, fertility, and efficiency of hybridization through recurrent and congruity backcrossing

Abstract

Cultivated common bean (Phaseolus vulgaris L.) and tepary bean (Phaseolus acutifolius A. Gray) genotypes possessing desirable agronomic traits were hybridized. The F1 hybrids were backcrossed twice with the common bean (i.e., recurrent backcrossing). Also, alternate backcrosses with common and tepary beans (i.e., congruity backcrossing) were carried out. Embryo culture was necessary for all initial interspecific crosses, and its requirement was proportionally lower when the common bean was used as the recurrent parent and as the last parent of congruity backcrosses. Modification of the embryo culture technique was necessary to produce congruity hybrids. Effects of both tepary and common bean genotypes on the success rate of hybridization were observed. Tepary accession G 40001 and common bean cultivar ICA Pijao facilitated interspecies hybridization. Growth of hybrid embryos before rescue, recovery of mature hybrid plants, and the vigor and fertility of F1 hybrids all increased with increased recurrent and congruity backcrosses and intermatings between male-sterile F1 and selected fertile F2 plants of the third and fifth congruity backcrosses. Introgression of tepary genes was verified by means of seed protein electrophoretic analysis and morphological markers. The results suggest that congruity backcrossing can help to gradually reduce or overcome P. vulgaris x P. acutifolius hybridization barriers such as genotype incompatibility, early embryo abortion, hybrid sterility, and lower frequencies of hybridization.

This is a preview of subscription content, access via your institution.

References

  1. Alvarez MN, Ascher PD, Davis DW (1981) Interspecific hybridization in Euphaseolus through embryo rescue. Hort Sci 16:541–543

    Google Scholar 

  2. Bhojwani SS, Razdan MK (1983) Plant tissue culture: theory and practice Elsevier Science Publishers, Amsterdam, Netherlands

    Google Scholar 

  3. Bliss FA (1980) Common bean. In: Fehr WR, Hadley HH (eds), Hybridization of crop plants. Am Soc Agron, Madison, Wisconsin, pp 273–284

    Google Scholar 

  4. Buishand TJ (1956) The crossing of bean (Phaseolus spp.). Euphytica 5:41–50

    Google Scholar 

  5. Coyne DP (1964) Species hybridization in Phaseolus. J Hered 55:5–6

    Google Scholar 

  6. Gamborg OL, Miller RA, Ojina K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    CAS  PubMed  Google Scholar 

  7. Haghighi KR, Ascher PD (1988) Fertile, intermediate hybrids between Phaseolus vulgaris and P. acutifolius from congruity back-crossing. Sex Plant Reprod 1:51–58

    Google Scholar 

  8. Honma S (1955) A technique for artificial culturing of bean embryos. Proc Am Soc Hort Sci 72:405–408

    Google Scholar 

  9. Honma S (1956) A bean interspecific hybrid. J Hered 47:217–220

    Google Scholar 

  10. Hussain A, Ramirez H, Bushuk W, Roca W (1986) Field bean (Phaseolus vulgaris L.) cultivar identification by electrophoregrams of cotyledon storage proteins. Euphytica 35:729–732

    Google Scholar 

  11. McElroy JB (1985) Breeding dry beans, Phaseolus vulgaris L., for common bacterial blight resistance derived from Phaseolus acutifolius A. Gray. PhD dissertation, Cornell University, Ithaca, New York

    Google Scholar 

  12. Mok DWS, Mok MC, Rabakoarihanta A (1978) Interspecific hybridization of Phaseolus vulgaris with P. lunatus and P. acutifolius. Theor Appl Genet 52:209–215

    Google Scholar 

  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  14. Parker JP, Michaels TE (1986) Simple genetic control of hybrid plant development in interspecific crosses between Phaseolus vulgaris L. and P. acutifolius A. Gray. Plant Breed 97:315–323

    Google Scholar 

  15. Parsons LR, Howe TK (1984) Effects of water stress on the water relations of Phaseolus vulgaris and the drought resistant Phaseolus acutifolius. Physiol Plant 60:197–202

    Google Scholar 

  16. Pratt RC (1983) Gene transfer between tepary and common beans. Desert Plants 5:57–63

    Google Scholar 

  17. Pratt RC, Bressan RA, Hasegawa PM (1985) Genotypic diversity enhances recovery of hybrids and fertile backcrosses of Phaseolus vulgaris L. tand P. acutifolius. A. Gray. Euphytica 34:329–344

    Google Scholar 

  18. Prendota K, Baudoin JP, Marechal R (1982) Fertile allopolyploids from the cross Phaseolus acutifolius x Phaseolus vulgaris. Bull Rech Agron Gembloux 17:177–190

    Google Scholar 

  19. Pythoud F, Buchala AJ (1989) The fate of vitamin D3 and indolylbutyric acid applied to cuttings of Populus tremula L. during adventitious root formation. Plant Cell Environ 125:489–494

    Google Scholar 

  20. Scott ME, Michaels TE (1990) Genetic control of abnormal seedling development in Phaseolus vulgaris L. and P. vulgaris x P. acutifolius A. Gray hybrids. Plant Breed 104:102–107

    Google Scholar 

  21. Singh SP (1992) Common bean improvement in the tropics. Plant Breed Rev 10:199–269

    Google Scholar 

  22. Singh SP, Gutiérrez JA (1984) Geographical distribution of Dl1 and Dl2 genes causing hybrid dwarfism in Phaseolus vulgaris L., their association with seed size, and their significance to breeding. Euphytica 33:337–345

    Google Scholar 

  23. Singh SP, Gepts P, Debouck DG (1991) Races of common bean (Phaseolus vulgaris, Fabaceae). Econ Bot 45:379–396

    Google Scholar 

  24. Smartt J (1970) Interspecific hybridization between cultivated American species of the genus Phaseolus. Euphytica 19:480–489

    Google Scholar 

  25. Stockinger EJ, Waines JG, Italuya FM (1986) Interspecific hybridization between Kenyan common beans and tepary beans. Annu Rep Bean Improv Coop 29:93–94

    Google Scholar 

  26. Thomas CV, Waines JG (1984) Fertile backcross and allotetraploid plants from crosses between tepary beans and common beans. J Hered 75:93–98

    Google Scholar 

  27. Thomas CV, Manshardt RM, Waines JG (1983) Teparies as a source of useful traits for improving common beans. Desert Plants 5:43–48

    Google Scholar 

  28. Waines JG, Manshardt RM, Wells WC (1988) Interspecific hybridization between Phaseolus vulgaris and P. acutifolius. In: Gepts P (ed) Genetic resources of Phaseolus beans. Kluwer Academic Publishers, Dordrecht, Netherlands, pp 485–502

    Google Scholar 

  29. Zaiter HZ, Coyne DP, Vidaver AK, Steadman JR (1989) Differential reaction of tepary bean lines to Xanthomonas campestris pv phaseoli. Hort Sci 24:134–137

    Google Scholar 

Download references

Author information

Affiliations

Authors

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mejía-Jiménez, A., Muñoz, C., Jacobsen, H.J. et al. Interspecific hybridization between common and tepary beans: increased hybrid embryo growth, fertility, and efficiency of hybridization through recurrent and congruity backcrossing. Theoret. Appl. Genetics 88, 324–331 (1994). https://doi.org/10.1007/BF00223640

Download citation

Key words

  • Phaseolus acutifolius
  • Phaseolus vulgaris
  • Common and tepary beans
  • Interspecific hybridization
  • Congruity and recurrent backcrosses
  • In-vitro embryo culture
  • Gene introgression