Summary
With the use of the unlabeled antibody peroxidase-antiperoxidase (PAP) technique at the light microscopic level, it was shown that the preoptico-hypophysial neurosecretory system of the adult migrating Lampetra fluviatilis is a vasotocinergic system. It does not synthesize vasopressin. The results are entirely consistent with earlier chromatographic and pharmacological indications that it produces little or no oxytocin-like peptide hormone. In the adenohypophysis, immunoreactive neurohypophysial peptidergic fibres are absent.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ball, J.N., Baker, B.I.: The pituitary gland. Anatomy and histophysiology. In: Fish physiology. Vol. II, The endocrine system (W.S. Hoar and D.J. Randall, eds.), pp. 1–110. New York-London: Academic Press 1969
Bargmann, W.: Über das Zwischenhirn-Hypophysensystem von Fischen. Z. Zellforsch. 38, 275–298 (1953)
Dierickx, K., Vandesande, F.: Immuno-enzyme cytochemical demonstration of mesotocinergic nerve fibres in the pars intermedia of the amphibian hypophysis. Cell Tiss. Res. 174, 25–34 (1976)
Dierickx, K., Vandesande, F., De Mey, J.: Identification, in the external region of the rat median eminence, of separate neurophysin-vasopressin and neurophysin-oxytocin containing nerve fibres. Cell Tiss. Res. 168, 141–151 (1976)
Dodd, J.M., Follett, B.K., Sharp, P.J.: Hypothalamic control of pituitary function in submammalian vertebrates. In: Advances in comparative physiology and biochemistry. Vol. 4 (O. Lowenstein, ed), pp. 113–223. New York-London: Academic press 1971
Follett, B.K., Heller, H.: The neurohypophysial hormones of bony fishes and cyclostomes. J. Physiol. (Lond.) 172, 74–91 (1964)
Goossens, N., Dierickx, K., Vandesande, F.: Immuno-cytochemical localization of vasotocin and isotocin in the preoptico-hypophysial neurosecretory system of teleosts. Gen. comp. Endocr. 1976 (in press)
Holmes, R.L., Ball, J.N.: The pituitary gland. A comparative account. Cambridge: University Press 1974
Moriarty, G.C.: Adenohypophysis: Ultrastructural cytochemistry. A review. J. Histochem. Cyto 21, 855–894 (1973)
Moriarty, G.C., Moriarty, C.M., Sternberger, L.A.: Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase-antiperoxidase complex. A technique more sensitive than radioimmunoassay. J. Histochem. Cytochem. 21, 825–833 (1973)
Öztan, N., Gorbman, A.: The hypophysis and hypothalamo-hypophysial neurosecretory system of larval lampreys and their responses to light. J. Morph. 106, 243–261 (1960)
Perks, A.M.: The neurohypophysis. In: Fish physiology. Vol. II, The endocrine system (W.S. Hoar and D.J. Randall, eds.), pp. 111–205. New York-London: Academic Press 1969
Polenov, A.L., Belenky, M.A., Konstantinova, M.S.: The hypothalamo-hypophysial system of the lamprey, Lampetra fluviatilis L. I. The neurohypophysis. Cell Tiss. Res. 150, 505–519 (1974)
Roth, W.D.: Some evolutionary aspects of neurosecretion in the sea lamprey, Petromyzon marinus. Anat. Rec. 124, 437 (1956)
Rühle, H.-.J., Sterba, G.: Zur Histologie der Hypophyse des Flussneunauges (Lampetra fluviatilis L.). Z. Zellforsch. 70, 136–168 (1966)
Sawyer, W.H.: Active neurohypophysial principles from a cyclostome (Petromyzon marinus) and two cartilaginous fishes (Squalus acanthias and Hydrolagus collei). Gen. comp. Endocr. 5, 427–439 (1965)
Sawyer, W.H.: Phylogenetic aspects of the neurohypophysial hormones. In: Handbook of experimental pharmacology. Neurohypophysial hormones and similar polypeptides (O. Eichler, A. Farah, H. Herken and A.D. Welch eds.), pp. 717–747. Berlin-Heidelberg-New York: Springer 1968
Sawyer, W.H., Munsick, R.A., van Dycke, H.B.: Pharmacological characteristics of the active principles in neurohypophysial extracts from several species of fishes. Endocrinol. 68, 215–225 (1961)
Scharrer, E.: Das Hypophysen-Zwischenhirn-System der Wirbeltiere. Anat. Anz., Erg.-Bd. 100, 5–29 (1954)
Sterba, G.: Fluoreszenzmikroskopische Untersuchungen über die Neurosekretion beim Bachneunauge (Lampetra planeri Bloch). Z. Zellforsch. 55, 763–789 (1961)
Sternberger, L.A., Hardy, P.H., Jr., Cuculis, J.J., Meyer, H.G.: The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase—anti horseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)
Tsuneki, K., Gorbman, A.: Ultrastructure of the anterior neurohypophysis and the pars distalis of the lamprey, Lampetra tridentata. Gen. comp. Endocr. 25, 487–508 (1975a)
Tsuneki, K., Gorbman, A.: Ultrastructure of pars nervosa and pars intermedia of the lamprey, Lampetra tridentata. Cell Tiss. Res. 157, 165–184 (1975b)
Vandesande, F., Dierickx, K.: Identification of the vasopressin producing and of the oxytocin producing neurons in te hypothalamic magnocellular neurosecretory system of the rat. Cell Tiss. Res. 164, 153–162 (1975)
Vandesande, F., Dierickx, K.: Immunocytochemical demonstration of separate vasotocinergic and mesotocinergic neurons in the amphibian hypothalamic magnocellular neurosecretory system. Cell Tiss. Res. 175, 289–296 (1976)
Vandesande, F., Dierickx, K., De Mey, J.: Identification of the vasopressin-neurophysin II and the oxytocin-neurophysin I producing neurons in the bovine hypothalamus. Cell Tiss. Res. 156, 189–200 (1975)
Author information
Authors and Affiliations
Additional information
This investigation was supported by a grant from the Belgian Nationaal Fonds voor Geneeskundig Wetenschappelijk Onderzoek
Rights and permissions
About this article
Cite this article
Goossens, N., Dierickx, K. & Vandesande, F. Immunocytochemical demonstration of the hypothalamo-hypophysial vasotocinergic system of Lampetra fluviatilis . Cell Tissue Res. 177, 317–323 (1977). https://doi.org/10.1007/BF00220307
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00220307