Summary
Detergent insoluble material (DIM) was prepared by gentle treatment with detergent from foetal, regenerating and adult normal rat hepatocytes cultured for various times. It retained to some degree the morphology of the cells. After incubation of intact cells with 35S-methionine, most of the labelled DIM proteins were found to be components of the cytoskeleton. They included several cytokeratins, vimentin and actin. The synthesis rate varied with the age of animals and culture conditions. The high synthetic rate of vimentin in foetal and regenerating hepatocytes could be associated with cell proliferation. No correlation was found between cytokeratin synthesis and hepatocyte growth. Most of the cytoskeleton proteins could be phosphorylated in intact cells and in DIM from cultured hepatocytes. However the degree of phosphorylation of these proteins was not related to their synthetic rate. The decreased phosphorylation level in cultured adult rat hepatocytes could be related to the rapid loss of specific functions.
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References
Franke WW, Mayer D, Schmid E, Denk H, Borenfreund E: Exp Cell Res 134:345–365, 1981.
Lenk R, Ranson L, Kaufmann Y, Penman S: Cell 10:67–78, 1977.
Gacon G, Gisselbrecht S, Piau JP, Fiszman MY, Fischer S: Eur J Biochem 125:543–456,1982.
Seglen PO: Exp Cell Res 76:25–30, 1973.
Guguen C, Guillouzo A, Boisnard M, Le Cam A, Bourel M: Biol Gastroenterol 8:223–231, 1975.
Bonney RJ, Becker JE, Walker PR, Potter VR: In Vitro 9:399–413, 1974.
Guguen C, Gregori C, Schapira F: Biochimie 57:1065–1071, 1975.
Pariza WW, Yager JD Jr, Goldfarb S, Gurr JA, Yanachi S, Grossman SH, Becker JE, Barber TA, Potter VR: In Gerschenson LE and Thompson EB (eds), Gene expression and carcinogenesis in cultured liver. Academic Press, New-York, 1975, pp 137–167.
Guguen-Guillouzo C, Tichonicky L, Szajnert MF, Schapira F, Kruh J: Biol Cell 31:225–234, 1978.
Krebs EG, Beavo JA: Ann Rev Biochem 48: 923–959, 1979.
Baffet G, Clement B, Glaise D, Guillouzo A, Guguen-Guillouzo C: Biochem Biophys Res Commun 109:507–512, 1982.
Guguen-Guillouzo C, Clement B, Baffet G, Beaumont C, Morel-Chany E, Glaise D, Guillouzo A: Exp Cell Res 143:47–54, 1983.
Guguen-Guillouzo C, Tichonicky L, Szajnert MF, Kruh J: In Vitro 16:1–10, 1980.
Leffert HL, Paul D: J Cell Biol 52:559–568, 1972.
Laemmli UK: Nature 227:680–685, 1970.
O'Farrell P: J Biol Chem 250:4007–4021, 1975.
Cervera M, Dreyfuss G, Penmann S: Cell 23:113–120, 1981.
Hunter T, Sefton BM: In Cohen and Van Heyningen (eds) Molecular Action of Toxins and Viruses Vol. 9, pp 333–366. Elsevier Biomedical Press, 1982.
Mak WW-N, Sattler CA, Pitot HC: Cancer Res 40: 4552–4564, 1980.
Penmann S, Fulton A, Capco D, Ben Ze'ev A, Wittelsberger S, Tse CF: Cold Spring Harbor Symp Quant Biol 46: 1013–1028,1981.
Denk H, Franke WW: In Arias I, Popper H, Schachter D and Shafritz DA (eds) The Liver. Raven Press, New-York, 1982, pp 55–71.
Connell DN, Rheinwald JG: Cell 34:245–253, 1983.
Venetianer A, Schiller DL, Magin T, Franke WW: Nature 305:730–733, 1983.
Sirica AE, Richards W, Tsukada Y, Sattler CA, Pitot HC: Proc Natl Acad Sci USA76:283–287, 1979.
Guzelian PS, Bissell DM, Meyer UA: Gastroenterology 72:1232–1239, 1977.
Clayton DF, Darnell JE: Mol Cell Biol 3:1552–1561, 1983.
Guguen-Guillouzo C, Szajnert MF, Glaise D, Gregori C, Schapira F: In vitro 10:206–214, 1980.
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Baffet, G., Ruelland, A., Clement, B. et al. Synthesis and phosphorylation of cytoskeleton components in foetal, regenerating and adult normal rat hepatocytes during culture. Mol Cell Biochem 68, 97–105 (1985). https://doi.org/10.1007/BF00219373
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DOI: https://doi.org/10.1007/BF00219373