Skip to main content
SpringerLink
Log in

Immunocytochemical localization and spatial relation to the adenohypophysis of a somatostatin-like and a corticotropin-releasing factor-like peptide in the brain of four amphibian species

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The distribution of somatostatin (SRIF) — and corticotropin-releasing factor (CRF)-like — immunoreactive material was studied in the brain of four amphibian species (Ambystoma mexicanum, Pleurodeles waltlii, Xenopus laevis, Rana ridibunda) by use of immunocytochemistry. A wide network of SRIF-immunoreactive fibers and numerous perikarya were observed in all amphibians examined, with a dense accumulation of nerve endings in the external layer of the median eminence (ELME). In the representatives of the four amphibian species the CRF-like system was more circumscribed. Immunoreactive perikarya were present in the preoptic area, mainly in a ventrobasal position, and in the interpeduncular nucleus. The tract running along the ventral part of the tuber cinereum ends in the ELME facing the rostroventral lobe of the pars distalis that contains corticotrophs. CRF fibers were scarce or absent in the neural lobe. In all species studied in the present work, CRF fibers end in the area of the ELME close to the pituitary lobe containing corticotrophs. This correlation is similar to that reported for the Japanese quail and several teleosts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andrieux B, Dubois MP, Dacheux F (1977) Localisation et identification des types cellulaires de l'hypophyse distale du triton Pleurodeles waltlii Michah à l'aide des techniques d'immunofluorescence, d'immunochimie ultrastructurale et de microscopie photonique et électronique. Biol Cell 29:135–148

    Google Scholar 

  • Bear MF, Ebner FF (1983) Somatostatin-like immunoreactivity in the forebrain of Pseudemys turtles. Neuroscience 9:297–308

    Google Scholar 

  • Blähser S, Vigh-Teichmann I, Ueck M (1982) Cerebrospinal fluid-contacting neurons and other somatostatin-immunoreactive perikarya in brains of tadpoles of Xenopus laevis. Cell Tissue Res 224:693–697

    Google Scholar 

  • Bons N, Bouillé C, Vaudry H, Guillaume V (1985) Localisation par immunofluorescence des neurones à corticolibérine dans l'encéphale de Pigeon. CR Acad Sci Paris 300 ser. III:49–52

    Google Scholar 

  • Bugnon C, Fellmann D, Gouget A, Bresson JL, Clavequin MC, Hadjiyiassemis M, Cardot J (1984) Corticoliberin neurons: cytophysiology, phylogeny and ontogeny. J Steroid Biochem 20:183–195

    Google Scholar 

  • Cuet P, Burlet A, Jégou S, Pelletier G, Vaudry H, Tonon MC (1984) CRF stimulates specifically the pars distalis of the frog pituitary gland. Gen Comp Endocrinol 53:438–439

    Google Scholar 

  • De Souza EB, Perrin MH, Insel TR, Rivier J, Vale WW, Kuhar MJ (1984) Corticotropin-releasing factor receptors in rat forebrain: auto-radiographic identification. Science 224:1449–1451

    Google Scholar 

  • Dierickx K (1965) A gradient of activity in the hypophysis of Rana temporaria. Z Zellforsch 65:514–520

    Google Scholar 

  • Dierickx K, Goossens N, Vandesande F (1981) The origin of somatostatin fibers in the median eminence and neural lobe of Rana temporaria. Cell Tissue Res 215:41–46

    Google Scholar 

  • Doerr-Schott J (1972) Les cellules corticotropes de l'hypophyse d'un amphibien après interrénalectomie. Etude cytoimmunochimique et ultrastructurale. Z Zellforsch 132:333–346

    Google Scholar 

  • Doerr-Schott J (1974a) Cyto-immunochemical study of the hypophysial cells of amphibians by light- and electron-microscopy. Fortsch Zool 22:245–267

    Google Scholar 

  • Doerr-Schott J (1974b) Localisation submicroscopique par cytoimmunoenzymologie de différents principes hormonaux de l'hypophyse de Rana temporaria. J Microsc 20:151–164

    Google Scholar 

  • Doerr-Schott J, Dubois MP (1976) Répartition comparée de structures réagissant en immunofluorescence avec des anticorps, anti-somatostatine, anti-neurophysine et anti-LH-RH chez deux Amphibiens: le crapaud et le xénope. J Physiol (Paris) 72:5B

    Google Scholar 

  • Doerr-Schott J, Dubois MP (1977) Immunohistochemical demonstration of a SRIF-like system in the brain of the reptile: Lacerta muralis Laur. Experientia 33:947–949

    Google Scholar 

  • Doerr-Schott J, Garaud JC, Clauss RO (1979) Immunohistochemical localization of a gastrin-like peptide in the brain of an amphibian, Xenopus laevis, Daud. Cell Tissue Res 203:65–78

    Google Scholar 

  • Dubois MP, Barry J, Leonardelli J (1974) Mise en evidence par immunofluorescence et répartition de la somatostatine (SRIF) dans l'éminence médiane des Vertébrés (Mammifères, Oiseaux, Amphibiens, Poissons). CR Acad Sci Paris 279D:1899–1902

    Google Scholar 

  • Fasolo A, Gaudino G (1981) Somatostatin immunoreactive neurons and fibers in the hypothalamus of the newt. Gen Comp Endocrinol 44:256–263

    Google Scholar 

  • Fasolo A, Gaudino G (1982) Immunohistochemical localization of somatostatin-like immunoreactivity in the hypothalamus of the lizard, Lacerta sicula. Gen Comp Endocrinol 48:205–212

    Google Scholar 

  • Fasolo A, Andreone C, Vandesande F (1984a) Immunohistochemical localization of corticotropin-releasing factor (CRF)-like immunoreactivity in the hypothalamus of the newt, Triturus cristatus. Neurosci Lett 49:135–142

    Google Scholar 

  • Fasolo A, Renda T, Melchorri P, Vandesande F, Mazzi V (1984b) Sauvagine and CRF-like immunoreactivities in the brain of the newt. Neurosci Lett [suppl] 18:S157

    Google Scholar 

  • Fryer J, Lederis K (1984) Urotensin I and corticotropin secretion: comparative actions in fishes and mammals. 9th Internat Symp Neurosecretion, Japan 48

  • Gaudino G, Fasolo A (1980) Substance P-related peptides in the hypothalamums of Amphibia. Cell Tissue Res 211:241–250

    Google Scholar 

  • Giraud P, Gillioz P, Conte-Devolx B, Oliver C (1979) Distribution de thyroliberine (TRH), α-melanocyte-stimulating hormone (α-MSH) et somatostatine dans les tissus de la grenouille verte (Rana esculenta). CR Acad Sci Paris 288D:127–129

    Google Scholar 

  • Girod C (1977) Apport de l'immunohistochimie à l'étude cytologique de l'adénohypophyse. Bull Assoc Anat 62:417–603

    Google Scholar 

  • Goossens N, Dierickx K, Vandesande F (1980) Immunocytochemical localization of somatostatin in the brain of the lizard, Ctenosauria pectinata. Cell Tissue Res 208:499–505

    Google Scholar 

  • Hauser-Gunsbourg N, Doerr-Schott J, Dubois MP (1973) Localisation par immunocytologie de secrétions apparentées aux hormones thyréotropes, gonadotropes et corticotropes dans l'hypophyse distale de l'axolotl (Ambystoma mexicanum, Shaw). Z Zellforsch 142:539–548

    Google Scholar 

  • Hauser-Gunsbourg N, Doerr-Schott J, Dubois MP (1978) Identification by immunofluorescence of prolactin and somatotropin producing cells in the pituitary gland of the Mexican axolotl (Ambystoma mexicanum, Shaw). Cell Tissue Res 194:497–502

    Google Scholar 

  • Józsa R, Vigh S, Schally AV, Mess B (1984) Localization of corticotropin-releasing factor containing neurons in the brain of the domestic fowl. An immunohistochemical study. Cell Tissue Res 236:245–248

    Google Scholar 

  • Kah O, Chambolle P, Dubourg P, Dubois MP (1982) Localisation immunocytochimique de la somatostatine dans le cerveau antérieur et l'hypophyse de deux Téléostéens, le Cyprin (Carassius auratus) et Gambusia sp. CR Acad Sci Paris 294 ser. 111:519–524

    Google Scholar 

  • Kobayashi Y, Woo NYS, Ishimura K, Poulin P, Hontela A, Lederis K (1986) Immunoreactive urotensin I, a corticotropin-releasing factor-like peptide, in fish brain. In: Yoshimura F, Gorbman A (eds) Pars Distalis of the Pituitary Gland. Structure, Function and Regulation. Elsevier Sci Publ Amsterdam, 267–269

    Google Scholar 

  • Larsen LO, Van Kemenade JAM, Van Dongen WJ (1971) Localization and identification of corticotropin-producing cells in the distal lobe of the Rana temporaria pituitary gland. Gen Comp Endocrinol 16:165–168

    Google Scholar 

  • Mikami S (1986) Immunocytochemistry of the avian hypothalamus and adenohypophysis. Int Rev Cytol 103:189–248

    Google Scholar 

  • Olivereau M, Bugnon C, Fellmann D, Olivereau JM, Aimar C (1976) Etude cytoimmunologique de l'hypophyse du Pleurodéle. CR Soc Biol 170:1147–1151

    Google Scholar 

  • Olivereau M, Vandesande F, Boucique E, Ollevier F, Olivereau JM (1984a) Mise en évidence immunocytochimique d'un système peptidergique de type CRF (corticotropin-releasing factor) dans le cerveau des Amphibiens. Comparaison avec la répartition du système à somatostatine. CR Acad Sci Paris 299 ser. III:871–876

    Google Scholar 

  • Olivereau M, Ollevier F, Vandesande F, Verdonck W (1984b) Immunocytochemical identification of CRF-like and SRIF-like peptides in the brain and the pituitary of cyprinid fish. Cell Tissue Res 237:379–382

    Google Scholar 

  • Olivereau M, Ollevier F, Vandesande F, Olivereau J (1984c) Somatostatin in the brain and the pituitary of some teleost fishes. Immunocytochemical identification and the effect of starvation. Cell Tissue Res 238:289–296

    Google Scholar 

  • Oota Y, Kobayashi H, Nishioka RS, Bern HA (1974) Relationship between neurosecretory axon and ependymal terminals on capillary walls in the median eminence of several vertebrates. Neuroendocrinology 16:127–136

    Google Scholar 

  • Ortman R, Sirett NE, Cohen KH, Druker M (1972) Bioassay studies of rostral and caudal halves of the pars distalis of Rana pipiens for adrenocorticotropin and gonadotropin. Gen Comp Endocrinol 19:325–330

    Google Scholar 

  • Panzica GC, Viglietti-Panzica C, Fasolo A, Franzoni MF, Vandesande F (1984) Corticotropin-releasing factor (CRF)-like system in the quail prosencephalon. Neurosci Lett [suppl] 18:S157

    Google Scholar 

  • Pearson D (1984) Biosynthesis of urotensins in the caudal neurosecretory complex of fishes. 9th Internat Symp Neurosecretion, Japan 47

  • Péczely P, Antoni FA (1984) Comparative localization of neurons containing ovine corticotropin-releasing factor (CRF)-like and neurophysin-like immunoreactivity in the diencephalon of the pigeon (Columba livia domestica). J Comp Neurol 228:69–80

    Google Scholar 

  • Rémy C, Dubois MP (1978) Immunofluorescence of somatostatin-producing sites in the hypothalamus of the tadpole, Alytes obstetricans Laur. Cell Tissue Res 187:315–321

    Google Scholar 

  • Seki T, Nakai Y, Shioda S, Mitsuma T, Kikuyama S (1983) Distribution of immunoreactive thyrotropin-releasing hormone in the forebrain and hypophysis of the bullfrog, Rana catesbeiana. Cell Tissue Res 233:507–516

    Google Scholar 

  • Shapiro B, Sheppard M, Kronheim S, Pimstone BL (1979) Tissue distribution of immunoreactive somatostatin in the South African clawed toad (Xenopus laevis). J Endocrinol 80:407–408

    Google Scholar 

  • Skowsky WR, Fisher DA (1972) The use of thyroglobulin to induce antigenicity to small molecules. J Lab Clin Med 80:134–144

    Google Scholar 

  • Tonon MC, Burlet A, Lauber M, Cuet P, Jégou S, Gouteux L, Ling N, Vaudry H (1985) Immunohistochemical localization and radioimmunoassay of corticotropin-releasing factor in the forebrain and hypophysis of the frog Rana ridibunda. Neuroendocrinology 40:109–119

    Google Scholar 

  • Tonon MC, Cuet P, Lamacz M, Jegou S, Cote J, Gouteux L, Ling N, Pelletier G, Vaudry H (1986) Comparative effects of corticotropin-releasing factor, arginine vasopressin, and related neuropeptides on the secretion of ACTH and α-MSH by frog anterior pituitary cells and neurointermediate lobes in vitro. Gen Comp Endocrinol 61:438–445

    Google Scholar 

  • Vandesande F, Dierickx K (1976) Immunocytochemical demonstration of separate vasotocinergic and mesotocinergic neurons in the amphibian hypothalamic magnocellular neurosecretory system. Cell Tissue Res 175:289–296

    Google Scholar 

  • Vandesande F, Dierickx K (1980) Immunocytochemical localization of somatostatin-containing neurons in the brain of Rana temporaria. Cell Tissue Res 205:43–53

    Google Scholar 

  • Vandesande F, Dierickx K, De Mey J (1977) The origin of the vasopressinergic and oxytocinergic fibers of the external region of the median eminence of the rat hypophysis. Cell Tissue Res 180:443–452

    Google Scholar 

  • Van Kemenade JAM (1969) The effects of metopirone and aldactone on the pars distalis of the pituitary, the interrenal tissue and the interstitial tissue of the testis in the common frog, Rana temporaria. Z Zellforsch 96:466–477

    Google Scholar 

  • Vaudry H, Dupont W, Leboulenger F (1972) Mise en évidence des cellules corticotropes de l'hypophyse de la grenouille verte Rana esculenta L., par la technique d'immunofluorescence. CR Soc Biol 166:1362–1365

    Google Scholar 

  • Vaudry H, Leboulenger F, Trochard MC, Vaillant R (1977) Changes in corticotropin producing cells in the pituitary of Rana esculenta L. following interrenalectomy and metopirone treatment. An immunohistochemical study. Gen Comp Endocrinol 32:78–88

    Google Scholar 

  • Vigh-Teichmann I, Vigh B, Korf HW, Oksche A (1983) CSF-contacting and other somatostatin-immunoreactive neurons in the brains of Anguilla anguilla, Phoxinus phoxinus and Salmo gairdneri (Teleostei). Cell Tissue Res 233:319–334

    Google Scholar 

  • Weindl A, Triepel J, Kuchling G (1984) Somatostatin in the brain of the turtle Testudo hermanni Gmelin. An immunohistochemical mapping study. Peptides [suppl] 5:91–100

    Google Scholar 

  • Westlund KN, Chmielowiec S, Childs GV (1983) Somatostatin fibers and their relationship to specific cell types (GH and TSH) in the rat anterior pituitary. Peptides 4:557–562

    Google Scholar 

  • Yui R (1983) Immunohistochemical studies on peptide neurons in the hypothalamus of the bullfrog Rana catesbeiana. Gen Comp Endocrinol 49:195–209

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Physiologie, Institut Océanographique, Paris, France

    M. Olivereau

  2. Zoological Institute, Leuven, Belgium

    F. Vandesande, E. Boucique & F. Ollevier

  3. Université René Descartes, Clichy, France

    J. M. Olivereau

Authors
  1. M. Olivereau
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Vandesande
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Boucique
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. Ollevier
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. M. Olivereau
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by a grant from the Belgian Nationaal Fonds voor Geneeskundig Wetenschappelijk Onderzoek and the CNRS

Rights and permissions

Reprints and permissions

About this article

Cite this article

Olivereau, M., Vandesande, F., Boucique, E. et al. Immunocytochemical localization and spatial relation to the adenohypophysis of a somatostatin-like and a corticotropin-releasing factor-like peptide in the brain of four amphibian species. Cell Tissue Res. 247, 317–324 (1987). https://doi.org/10.1007/BF00218313

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00218313

Key words

Search

Navigation